微電流電解對(duì)藻類(lèi)生長(zhǎng)的抑制機(jī)理研究
發(fā)布時(shí)間：2019-02-12 22:35
【摘要】：保護(hù)湖泊、治理藍(lán)藻水華是當(dāng)今國(guó)內(nèi)外的重要課題。已有研究表明,微電流電解法作為一種電化學(xué)處理技術(shù),可利用電極的直接氧化以及電解產(chǎn)生的活性物質(zhì)的間接氧化作用有效抑制水體中藻類(lèi)的生長(zhǎng),并且對(duì)水中其它組分影響小,不會(huì)造成二次污染。本文以銅綠微囊藻為代表性藻類(lèi),采用微電流電解技術(shù)對(duì)藻類(lèi)的生長(zhǎng)進(jìn)行有效抑制,探究了微電流電解有效抑藻的最優(yōu)條件,研究了微電流電解對(duì)藻類(lèi)生理生態(tài)特征的影響和電解間接氧化作用的抑藻效能,從多角度闡釋了微電流電解抑藻的作用機(jī)理。主要研究結(jié)論如下:(1)比較了不同電極材料組合下微電流電解抑藻效果的差異,發(fā)現(xiàn)陽(yáng)極材料對(duì)電解抑藻效率的影響較大,陰極材料對(duì)抑藻效率影響較小,選擇了釕鈦和不銹鋼分別作為陽(yáng)極和陰極材料。探究了微電流電解抑藻的主要影響因素,發(fā)現(xiàn)在一定范圍內(nèi),電流密度、電解時(shí)間以及CaCl2濃度的增加均有助于提高電解抑藻效率,而電解液中Na2CO3以及MgSO4濃度的升高則會(huì)在一定程度上降低電解抑藻效率。采用BG-11培養(yǎng)基配制體積100ml、初始濃度為5×105個(gè)/mL的銅綠微囊藻液,以釕鈦、不銹鋼分別作陽(yáng)陰極,以10mA/cm2的電流密度電解處理15 min即可實(shí)現(xiàn)對(duì)藻類(lèi)生長(zhǎng)的持續(xù)抑制。(2)通過(guò)研究銅綠微囊藻在不同電流密度下葉綠素?zé)晒鈪?shù)的變化,從生態(tài)生理特征角度揭示電解抑藻的作用機(jī)理。研究發(fā)現(xiàn)對(duì)于其他條件一定的藻液來(lái)說(shuō),抑藻存在相應(yīng)的臨界電流閾值。當(dāng)電流密度小于臨界值時(shí),電解對(duì)藻細(xì)胞的脅迫未超過(guò)藻的耐受能力,藻液可在后期培養(yǎng)中恢復(fù)光合活性。若電流密度大于臨界值,電解對(duì)藻的脅迫會(huì)超過(guò)藻的耐受能力,電解會(huì)破壞掉光合系統(tǒng)II的結(jié)構(gòu)并阻斷其與藻膽體之間的連接,使其無(wú)法進(jìn)行光合作用,最終導(dǎo)致藻細(xì)胞的死亡。(3)從電解的間接氧化作用角度出發(fā),驗(yàn)證了活性物質(zhì)的抑藻能力,發(fā)現(xiàn)微電流電解過(guò)程中確實(shí)產(chǎn)生了具有抑藻效果的活性物質(zhì),足量的活性物質(zhì)對(duì)銅綠微囊藻具有良好的抑制效果,可導(dǎo)致已受電解破壞的藻細(xì)胞不能進(jìn)行自身修復(fù)而徹底死亡。對(duì)不同條件下間接氧化作用的抑藻能力進(jìn)行了探究,研究發(fā)現(xiàn)在一定范圍內(nèi),Cl-濃度、電流密度以及電解時(shí)間的增加均有助于提高微電流電解間接氧化的抑藻效果。(4)檢測(cè)并比較了電解產(chǎn)生的活性物質(zhì)中活性氯與過(guò)氧化氫的生成量,發(fā)現(xiàn)在不同電流密度、電解時(shí)間或Cl-濃度下,活性物質(zhì)抑藻效率與活性氯生成量呈正相關(guān),而與過(guò)氧化氫生成量的相關(guān)性不強(qiáng)。進(jìn)一步對(duì)比有無(wú)各類(lèi)活性物質(zhì)作用時(shí)藻類(lèi)的生長(zhǎng)狀況,得知活性氯與過(guò)氧化氫都具有一定的抑藻能力,但活性氯的抑藻能力高于過(guò)氧化氫,在活性物質(zhì)抑藻過(guò)程中起主導(dǎo)作用。
[Abstract]:Protecting lakes and controlling cyanobacteria Shui Hua is an important subject at home and abroad. It has been shown that as a kind of electrochemical treatment technology, micro-current electrolysis can effectively inhibit algae growth by direct oxidation of electrodes and indirect oxidation of active substances produced by electrolysis. And it has little influence on other components in water, so it will not cause secondary pollution. In this paper, microcystis aeruginosa was used as the representative algae, and the microcurrent electrolysis technique was used to effectively inhibit the growth of algae. The effects of microcurrent electrolysis on the physiological and ecological characteristics of algae and the inhibition effect of indirect electrolytic oxidation on algae were studied. The mechanism of microcurrent electrolysis on algae inhibition was explained from many angles. The main conclusions are as follows: (1) the effect of microcurrent electrolysis on algae inhibition under different electrode combinations was compared. It was found that anode material had a great effect on the inhibition efficiency of algae electrolysis, and cathode material had little effect on algae inhibition efficiency. Ruthenium titanium and stainless steel were used as anode and cathode material respectively. It was found that the increase of current density, electrolysis time and CaCl2 concentration could improve the efficiency of algae inhibition. However, the increase of Na2CO3 and MgSO4 concentration in electrolyte will reduce the efficiency of algae inhibition to some extent. The solution of microcystis aeruginosa with initial concentration of 5 脳 105 / mL was prepared by using BG-11 medium. Ruthenium titanium and stainless steel were used as positive cathodes, respectively. Continuous inhibition of algae growth could be achieved by electrolytic treatment of 10mA/cm2 current density for 15 min. (2) the changes of chlorophyll fluorescence parameters of microcystis aeruginosa at different current densities were studied. The mechanism of electrolytic algal inhibition was revealed from the point of view of ecological physiological characteristics. It is found that there is a critical current threshold for algae inhibition for other algae under certain conditions. When the current density was less than the critical value, the stress of electrolysis to the algal cells did not exceed the tolerance ability of the algae, and the algae solution could recover the photosynthetic activity in the later culture. If the current density is greater than the critical value, electrolysis of algae will exceed the tolerance of algae, and electrolysis will destroy the structure of photosynthetic system II and block its connection with phycobilis, which makes it impossible to photosynthesis. Finally, the algal cells died. (3) from the point of view of indirect oxidation of electrolysis, the anti-algal ability of the active substances was verified, and it was found that the active substances with anti-algal effect were produced in the process of micro-current electrolysis. A sufficient amount of active substances can inhibit microcystis aeruginosa well, which can cause the cells destroyed by electrolysis to be unable to repair themselves and die completely. The inhibitory effect of indirect oxidation on algae under different conditions was investigated. It was found that the concentration of Cl- was within a certain range. The increase of current density and electrolysis time can improve the inhibition effect of microcurrent electrolysis indirect oxidation. (4) the amount of active chlorine and hydrogen peroxide produced by electrolysis is detected and compared. Under electrolysis time or Cl- concentration, the inhibitory efficiency of active substances was positively correlated with the amount of active chlorine, but not with the amount of hydrogen peroxide. Compared with the growth of algae with or without various active substances, it was found that both active chlorine and hydrogen peroxide had a certain ability to inhibit algae, but the inhibitory ability of active chlorine was higher than that of hydrogen peroxide, which played a leading role in the process of algal inhibition of active substances.
【學(xué)位授予單位】：長(zhǎng)江科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X52
本文編號(hào)：2420860
[Abstract]:Protecting lakes and controlling cyanobacteria Shui Hua is an important subject at home and abroad. It has been shown that as a kind of electrochemical treatment technology, micro-current electrolysis can effectively inhibit algae growth by direct oxidation of electrodes and indirect oxidation of active substances produced by electrolysis. And it has little influence on other components in water, so it will not cause secondary pollution. In this paper, microcystis aeruginosa was used as the representative algae, and the microcurrent electrolysis technique was used to effectively inhibit the growth of algae. The effects of microcurrent electrolysis on the physiological and ecological characteristics of algae and the inhibition effect of indirect electrolytic oxidation on algae were studied. The mechanism of microcurrent electrolysis on algae inhibition was explained from many angles. The main conclusions are as follows: (1) the effect of microcurrent electrolysis on algae inhibition under different electrode combinations was compared. It was found that anode material had a great effect on the inhibition efficiency of algae electrolysis, and cathode material had little effect on algae inhibition efficiency. Ruthenium titanium and stainless steel were used as anode and cathode material respectively. It was found that the increase of current density, electrolysis time and CaCl2 concentration could improve the efficiency of algae inhibition. However, the increase of Na2CO3 and MgSO4 concentration in electrolyte will reduce the efficiency of algae inhibition to some extent. The solution of microcystis aeruginosa with initial concentration of 5 脳 105 / mL was prepared by using BG-11 medium. Ruthenium titanium and stainless steel were used as positive cathodes, respectively. Continuous inhibition of algae growth could be achieved by electrolytic treatment of 10mA/cm2 current density for 15 min. (2) the changes of chlorophyll fluorescence parameters of microcystis aeruginosa at different current densities were studied. The mechanism of electrolytic algal inhibition was revealed from the point of view of ecological physiological characteristics. It is found that there is a critical current threshold for algae inhibition for other algae under certain conditions. When the current density was less than the critical value, the stress of electrolysis to the algal cells did not exceed the tolerance ability of the algae, and the algae solution could recover the photosynthetic activity in the later culture. If the current density is greater than the critical value, electrolysis of algae will exceed the tolerance of algae, and electrolysis will destroy the structure of photosynthetic system II and block its connection with phycobilis, which makes it impossible to photosynthesis. Finally, the algal cells died. (3) from the point of view of indirect oxidation of electrolysis, the anti-algal ability of the active substances was verified, and it was found that the active substances with anti-algal effect were produced in the process of micro-current electrolysis. A sufficient amount of active substances can inhibit microcystis aeruginosa well, which can cause the cells destroyed by electrolysis to be unable to repair themselves and die completely. The inhibitory effect of indirect oxidation on algae under different conditions was investigated. It was found that the concentration of Cl- was within a certain range. The increase of current density and electrolysis time can improve the inhibition effect of microcurrent electrolysis indirect oxidation. (4) the amount of active chlorine and hydrogen peroxide produced by electrolysis is detected and compared. Under electrolysis time or Cl- concentration, the inhibitory efficiency of active substances was positively correlated with the amount of active chlorine, but not with the amount of hydrogen peroxide. Compared with the growth of algae with or without various active substances, it was found that both active chlorine and hydrogen peroxide had a certain ability to inhibit algae, but the inhibitory ability of active chlorine was higher than that of hydrogen peroxide, which played a leading role in the process of algal inhibition of active substances.
【學(xué)位授予單位】：長(zhǎng)江科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X52
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