【摘要】：利用城市污水培養(yǎng)產(chǎn)油微藻,在凈化污水的同時生產(chǎn)生物柴油,既能緩解日趨嚴(yán)重的能源危機,又能減輕水體污染。微藻培養(yǎng)時提高其產(chǎn)量是整套微藻制備生物柴油工藝能夠順利運行的源頭保證,能夠有效降低成本。本論文的目的就是在戶外利用城市污水培養(yǎng)微藻,尋找出一套能夠最大限度地促進微藻生長的優(yōu)化組合方法,提高微藻生物柴油的經(jīng)濟可行性。本研究通過戶外實驗發(fā)現(xiàn)三種外加刺激方法(磁場、植物激素,細菌)對城市污水培養(yǎng)的蛋白核小球藻的生長,藻干重及油脂產(chǎn)量均有一定的促進作用,得出了每種促進方法使用的最適宜條件。研究了這三種外加刺激方法組合運用效果,通過正交實驗確定了戶外城市污水培養(yǎng)小球藻促進其生長及油脂積累的最佳組合方式。將最佳組合運用到戶外跑道池擴大化裝置中,在批次培養(yǎng)小球藻時,最佳組合使得小球藻的OD值提高了8.06%,葉綠素a的濃度提高了43.94%,藻干重提高了17.85%,油脂產(chǎn)量提高了27.54%;在半連續(xù)培養(yǎng)時,研究確定了跑道池藻液的最佳更新率為25%,小球藻的穩(wěn)定油脂產(chǎn)量為0.18 g/L,城市污水的多項污染物濃度均有所下降。本文還對外加刺激對小球藻的生長促進原因進行了初步探討,外加刺激使得小球藻的光合色素含量增加,提高了小球藻的光合作用,使得小球藻藻細胞中的抗氧化酶活性增加,促進了藻細胞生長,此外磁處理使得培養(yǎng)液的透光率增加,利于微藻吸收光能,光合細菌能改善培養(yǎng)液的p H,有效地改善了小球藻的生長環(huán)境,促進小球藻的生長。
[Abstract]:Using municipal sewage to cultivate oil-producing microalgae and produce biodiesel while purifying sewage can not only alleviate the serious energy crisis but also reduce water pollution. Increasing the production of microalgae is the source guarantee that the whole process of microalgae producing biodiesel can run smoothly and can effectively reduce the cost. The purpose of this paper is to cultivate microalgae in outdoor sewage, to find a set of optimal combination method which can promote the growth of microalgae to the maximum extent, and to improve the economic feasibility of microalgae biodiesel. In this study, it was found that three stimulation methods (magnetic field, phytohormone, bacteria) could promote the growth, dry weight and oil production of Chlorella Proteinuca cultured in municipal sewage. The optimum conditions for the use of each method are obtained. The combined effects of these three stimulation methods were studied, and the optimum combination of growth and oil accumulation of Chlorella vulgaris cultured with outdoor municipal sewage was determined by orthogonal experiment. Applying the best combination to the outdoor runway pool expansion device, the best combination increased the OD value, chlorophyll a concentration and dry weight of Chlorella vulgaris by 8.06, 43.94 and 17.85, respectively, in the batch culture of Chlorella vulgaris, which increased the OD value of Chlorella vulgaris by 8.06, the concentration of chlorophyll a by 43.94 and the dry weight of algae by 17.85. Oil yield increased 27.54%; During semi-continuous culture, the optimum rate of upgrading of algae solution in runway was determined to be 25, the stable oil production of Chlorella microphylla was 0.18 g / L, and the concentration of several pollutants in municipal sewage was decreased. The reasons for the growth of Chlorella vulgaris were also discussed. The photosynthetic pigment content of Chlorella vulgaris was increased, the photosynthesis of Chlorella vulgaris was increased, and the antioxidant enzyme activity of Chlorella vulgaris cells was increased. In addition, magnetic treatment increased the transmittance of the medium, which was beneficial to the absorption of light energy by microalgae, and photosynthetic bacteria could improve the growth environment of Chlorella vulgaris and promote the growth of Chlorella vulgaris.
【學(xué)位授予單位】：哈爾濱工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：Q949.2;X703

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