發(fā)布時(shí)間：2018-10-20 18:50

【摘要】：微生物溶藻是藻類水華控制的研究熱點(diǎn),但目前仍缺乏去除微生物發(fā)酵產(chǎn)物中營(yíng)養(yǎng)物質(zhì)的方法學(xué)研究,對(duì)野外水體采集的微囊藻的溶藻活性研究也較少見(jiàn)。比較了不同極性溶劑對(duì)溶藻物質(zhì)的固液萃取效率,研究了分子篩層析對(duì)溶藻物質(zhì)的純化效果,并測(cè)定了各提純步驟的中間產(chǎn)物對(duì)野外水體采集的銅綠微囊藻的溶藻效果。結(jié)果表明,在溶藻活性基本保持穩(wěn)定的前提下,固液萃取串聯(lián)分子篩層析技術(shù)對(duì)TOC、TN和TP的總?cè)コ势骄哌_(dá)97%,其中,固液萃取的分級(jí)去除率平均高達(dá)90%,而分子篩層析的分級(jí)去除率平均為70%,且分子篩層析后的產(chǎn)物對(duì)野外水體采集的銅綠微囊藻的去除率穩(wěn)定在60%左右。結(jié)果說(shuō)明,固液萃取與分子篩層析技術(shù)可以作為溶藻物質(zhì)工業(yè)化提取與實(shí)驗(yàn)室純化的重要方法。
[Abstract]:Algolysis by microorganism is a hot topic in the control of algae Shui Hua. However, there is still a lack of methodological research on the removal of nutrients from microbial fermentation products, and studies on the algae-lytic activity of microcystis collected from water bodies in the field are rare. The efficiency of solid-liquid extraction of algae-lytic substances with different polar solvents was compared. The purification effect of molecular sieve chromatography on algae-lysing substances was studied. The effect of the intermediate products of each purification step on algae dissolution of Microcystis aeruginosa collected in field water was determined. The results showed that the total removal efficiency of TOC,TN and TP by solid-liquid extraction series molecular sieve chromatography was as high as 97%, while the algae-lytic activity remained stable. The average removal rate of solid liquid extraction was as high as 90 and the average removal rate of molecular sieve chromatography was 70. The removal rate of microcystis aeruginosa collected in field water was about 60%. The results show that solid-liquid extraction and molecular sieve chromatography can be used as important methods for industrial extraction and laboratory purification of algae-soluble substances.
【作者單位】： 三峽大學(xué)三峽庫(kù)區(qū)生態(tài)環(huán)境教育部工程研究中心;湖北工業(yè)大學(xué)資源與環(huán)境工程學(xué)院河湖生態(tài)修復(fù)與藻類利用湖北省重點(diǎn)實(shí)驗(yàn)室;
【基金】：湖北省自然科學(xué)基金重點(diǎn)項(xiàng)目(2013CFA108) 三峽大學(xué)開(kāi)放基金項(xiàng)目(KF2013-06) 武漢市科技攻關(guān)項(xiàng)目(2014060101010061)
【分類號(hào)】：X172;X52

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