微生物同步異養(yǎng)硝化好氧反硝化脫氮技術(shù)研究
發(fā)布時(shí)間：2018-09-19 08:25
【摘要】：同步異養(yǎng)硝化好氧反硝化(Heterotrophic Nitrification and Aerobic Denitrification, SND)是一種新型生物脫氮技術(shù),不但可以同時(shí)去除污水中的氮和碳,且在反硝化過程中不必額外投加碳源,可實(shí)現(xiàn)在同一反應(yīng)器中完成硝化反硝化過程,將氨氮直接轉(zhuǎn)化為氣態(tài)氮化合物而排出,解決了傳統(tǒng)廢水處理工藝在經(jīng)濟(jì)適用方面與處理效率之間的矛盾,另外硝化反硝化過程中產(chǎn)生的部分酸和堿可相互中和,減少了緩沖劑的用量。但SND也存在一些問題,如難以在好氧反硝化過程中完全消耗異養(yǎng)硝化過程產(chǎn)物,易造成中間產(chǎn)物的積累,使得難以實(shí)現(xiàn)對(duì)運(yùn)行過程的控制。另外SND對(duì)高濃度氨氮含鹽廢水處理及其脫氮機(jī)理也有待進(jìn)一步探究。本文為獲得處理高濃度氨氮含鹽廢水的高效SND脫氮菌株,從鹽池底泥中分離篩選出一株SND脫氮菌株,進(jìn)行形態(tài)觀察16S rDNA分析鑒定,命名為Halomonas sp. K01�？疾炝颂荚捶N類、C/N、初始pH和氨氮濃度對(duì)Halomonas sp. K01 SND脫氮的影響：有機(jī)碳源丁二酸鈉有利于脫氮；適宜的C/N為5,；在初始pH 7.5-9這個(gè)范圍內(nèi)均呈現(xiàn)較高的脫氮率；當(dāng)氨氮濃度為10000 mg/L時(shí),12 d平均降解速率最高。表明菌株K01能夠以SND方式高效去除高濃度氨氮含鹽廢水中的氨氮。且脫氮過程中未檢測(cè)出硝酸根、亞硝酸根以及羥胺,說明沒有中間產(chǎn)物積累。Halomonas spK01對(duì)高濃度氨氮有較強(qiáng)耐受性,且具有較高的脫氮率和脫氮速率。對(duì)垃圾滲濾液主要成分進(jìn)行解析,探究了菌株K01與Z08在垃圾滲濾液中生長(zhǎng)及對(duì)垃圾滲濾液處理效果。Halomonas sp.K01是一株中度嗜鹽菌,具有耐鹽性,在10000 mg/L的高濃度氨氮下具有較高耐受性。能夠以同步異養(yǎng)硝化好氧反硝化方式高效脫氮,在高濃度氨氮廢水的凈化處理中具有重要意義。
[Abstract]:Simultaneous heterotrophic nitrification aerobic denitrification (Heterotrophic Nitrification and Aerobic Denitrification, SND) is a new biological denitrification technology, which can not only remove nitrogen and carbon from wastewater simultaneously, but also do not need additional carbon source in denitrification process. The process of nitrification and denitrification can be completed in the same reactor, and ammonia nitrogen can be directly transformed into gaseous nitrogen compounds and discharged. The contradiction between the economic application of traditional wastewater treatment process and the treatment efficiency is solved. In addition, some acids and bases produced during nitrification and denitrification can neutralize each other, reducing the amount of buffer. However, there are some problems in SND, such as it is difficult to completely consume the products of heterotrophic nitrification in aerobic denitrification process, and it is easy to cause the accumulation of intermediate products, which makes it difficult to control the operation process. In addition, the treatment and denitrification mechanism of high-concentration ammonia-nitrogen-containing wastewater by SND should be further explored. In order to obtain a high efficiency SND denitrification strain for the treatment of high concentration ammonia-nitrogen salt containing wastewater, a SND denitrification strain was isolated from the sediment of the salt pond and identified by morphological observation 16s rDNA analysis and named Halomonas sp.. K01. The carbon source species C / N, initial pH and ammonia-nitrogen concentration on Halomonas sp. were investigated. The effect of K01 SND nitrogen removal: organic carbon source sodium succinate is favorable for denitrification, the suitable C / N is 5, the denitrification rate is higher in the range of initial pH 7.5-9, and the average degradation rate is the highest in 12 days when the concentration of ammonia nitrogen is 10000 mg/L. The results showed that strain K01 could effectively remove ammonia nitrogen from high concentration wastewater containing ammonia nitrogen by SND. No nitrate, nitrite and hydroxylamine were detected during denitrification, which indicated that there was no intermediate product accumulation. Halomonas spK01 had strong tolerance to high concentration of ammonia nitrogen, and had higher denitrification rate and denitrification rate. The main components of landfill leachate were analyzed. The growth of strains K01 and Z08 in landfill leachate and the effect of treatment of landfill leachate. Halomonas sp.K01 was a moderately halophilic bacterium with salt tolerance. It has high tolerance at 10000 mg/L high concentration of ammonia nitrogen. High efficiency denitrification can be achieved by simultaneous heterotrophic nitrification and aerobic denitrification, which is of great significance in the purification and treatment of high concentration ammonia-nitrogen wastewater.
【學(xué)位授予單位】：大連海事大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X703;X172
本文編號(hào)：2249584
[Abstract]:Simultaneous heterotrophic nitrification aerobic denitrification (Heterotrophic Nitrification and Aerobic Denitrification, SND) is a new biological denitrification technology, which can not only remove nitrogen and carbon from wastewater simultaneously, but also do not need additional carbon source in denitrification process. The process of nitrification and denitrification can be completed in the same reactor, and ammonia nitrogen can be directly transformed into gaseous nitrogen compounds and discharged. The contradiction between the economic application of traditional wastewater treatment process and the treatment efficiency is solved. In addition, some acids and bases produced during nitrification and denitrification can neutralize each other, reducing the amount of buffer. However, there are some problems in SND, such as it is difficult to completely consume the products of heterotrophic nitrification in aerobic denitrification process, and it is easy to cause the accumulation of intermediate products, which makes it difficult to control the operation process. In addition, the treatment and denitrification mechanism of high-concentration ammonia-nitrogen-containing wastewater by SND should be further explored. In order to obtain a high efficiency SND denitrification strain for the treatment of high concentration ammonia-nitrogen salt containing wastewater, a SND denitrification strain was isolated from the sediment of the salt pond and identified by morphological observation 16s rDNA analysis and named Halomonas sp.. K01. The carbon source species C / N, initial pH and ammonia-nitrogen concentration on Halomonas sp. were investigated. The effect of K01 SND nitrogen removal: organic carbon source sodium succinate is favorable for denitrification, the suitable C / N is 5, the denitrification rate is higher in the range of initial pH 7.5-9, and the average degradation rate is the highest in 12 days when the concentration of ammonia nitrogen is 10000 mg/L. The results showed that strain K01 could effectively remove ammonia nitrogen from high concentration wastewater containing ammonia nitrogen by SND. No nitrate, nitrite and hydroxylamine were detected during denitrification, which indicated that there was no intermediate product accumulation. Halomonas spK01 had strong tolerance to high concentration of ammonia nitrogen, and had higher denitrification rate and denitrification rate. The main components of landfill leachate were analyzed. The growth of strains K01 and Z08 in landfill leachate and the effect of treatment of landfill leachate. Halomonas sp.K01 was a moderately halophilic bacterium with salt tolerance. It has high tolerance at 10000 mg/L high concentration of ammonia nitrogen. High efficiency denitrification can be achieved by simultaneous heterotrophic nitrification and aerobic denitrification, which is of great significance in the purification and treatment of high concentration ammonia-nitrogen wastewater.
【學(xué)位授予單位】：大連海事大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X703;X172
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 趙宗升,劉鴻亮,李炳偉,袁光鈺;高濃度氨氮廢水的高效生物脫氮途徑[J];中國(guó)給水排水;2001年05期
2 趙宗升,劉鴻亮,袁光鈺,李炳偉;A~2/O與混凝沉淀法處理垃圾滲濾液研究[J];中國(guó)給水排水;2001年11期
3 祝貴兵;彭永臻;郭建華;;短程硝化反硝化生物脫氮技術(shù)[J];哈爾濱工業(yè)大學(xué)學(xué)報(bào);2008年10期
4 朱明石;周少奇;;厭氧氨氧化—反硝化協(xié)同脫氮研究[J];化工環(huán)保;2008年03期
5 張樹軍;彭永臻;王淑瑩;鄭淑文;毛心慰;;城市生活垃圾晚期滲濾液中氨氮的常溫短程去除[J];化工學(xué)報(bào);2007年04期
6 譚亞軍,蔣展鵬,余剛;廢水處理催化濕式氧化法及其催化劑的研究進(jìn)展[J];環(huán)境工程;1999年04期
7 付迎春,錢仁淵,金鳴林;催化濕式氧化法處理氨氮廢水的研究[J];煤炭轉(zhuǎn)化;2004年02期
8 劉國(guó)躍;王昶昊;施云海;陳浩;;化學(xué)沉淀法處理高濃度氨氮廢水的實(shí)驗(yàn)研究[J];石油化工技術(shù)與經(jīng)濟(jì);2013年06期
9 茍莎;黃鈞;;異養(yǎng)硝化細(xì)菌脫氮特性及研究進(jìn)展[J];微生物學(xué)通報(bào);2009年02期
10 陸軼峰;城市污水生物脫氮除磷常規(guī)工藝分析[J];云南環(huán)境科學(xué);2002年01期
，本文編號(hào)：2249584
本文鏈接：http://sikaile.net/kejilunwen/huanjinggongchenglunwen/2249584.html
最近更新
教材專著
