【摘要】：利用微藻來處理水體富營養(yǎng)化污染,既有效率,又能節(jié)省成本。微綠球藻和小球藻均屬于單細(xì)胞微藻,易于培養(yǎng),并且繁殖較快,目前微綠球藻對其廢水處理效果及固定化研究較少。但是在單獨(dú)的藻類固定化系統(tǒng)中,藻體不能有效的利用其內(nèi)部的空間環(huán)境,菌藻共存系統(tǒng)克服了上述的缺點(diǎn),二者互利共生,增強(qiáng)了對氮磷的處理效果。本次試驗(yàn)以微綠球藻C170,小球藻C95及芽孢桿菌為研究對象,研究其在不同狀態(tài)下對氮、磷的去除效率,為污水處理生物固定化技術(shù)積累資料。研究結(jié)果如下:游離態(tài)微綠球藻(FN)和游離態(tài)小球藻(FC)對氮磷的去除效果及藻細(xì)胞的生長情況對比實(shí)驗(yàn)顯示:微綠球藻對氮磷的去除率(33.01%,30.5%)要高于小球藻對氮磷的去除率(25.4%,23.3%)。同時微綠球藻在污水中的生長率也快于小球藻。游離態(tài)微綠球藻(FN)、固定化微綠球藻(IN)、空白膠球(BB)對氮磷的去除效果及微藻生長情況對比實(shí)驗(yàn)顯示:固定化微綠球藻對氮磷的去除率(60.4%,76.6%)明顯高于游離微綠球藻對氮磷的去除率(30.5%,33%),海藻酸鈉包埋固定對微綠球藻沒有造成不可恢復(fù)的損傷,一旦環(huán)境適應(yīng)后會迅速恢復(fù)其活性。固定化菌藻(IBN)、固定化微綠球藻(IN)、固定化芽孢桿菌(IB)除氮磷對比實(shí)驗(yàn)證實(shí):固定化菌藻除氮磷效果最顯著分別為(60.4%,88.8%)明顯高于固定化微綠球藻對氮磷的去除率(49.5%,76.4%)與固定化芽孢桿菌對氮磷的去除率(31.7%,30%)。固定化菌藻混合后內(nèi)部環(huán)境有益于微綠球藻的生長,當(dāng)p H上升到最高點(diǎn)后,菌藻混合固定的p H變化最穩(wěn)定,且變化都在微綠球藻最適p H范圍之內(nèi)。藻菌分別以1:1、3:2、2:3混合固定后對氮磷的去除效果結(jié)果顯示:以1:1藻菌混合固定對氮、磷的去除率最好,分別為(60.4%、88.8%),菌藻相對對方最多的時候活性最大,對氮磷的去除效果最佳。
[Abstract]:Using microalgae to treat eutrophication pollution of water body can save both efficiency and cost. Chlorella microphylla and Chlorella microalgae both belong to single-celled microalgae, which are easy to culture and propagate quickly. At present, there are few studies on the effect and immobilization of Chlorella microphylla on wastewater treatment. But in a single algal immobilization system, algae can not effectively use its internal space environment, the algal coexistence system overcomes the above shortcomings, the two mutually beneficial symbiosis, enhance the treatment effect of nitrogen and phosphorus. In this experiment, the removal efficiency of nitrogen and phosphorus of Chlorella microphylla C170, Chlorella microphylla C95 and Bacillus spp were studied in different conditions, and the data were accumulated for the biological immobilization technology of sewage treatment. The results were as follows: the removal efficiency of nitrogen and phosphorus by free Chlorella microalgae (FN) and free Chlorella microalgae (FC) and the growth of algal cells were compared. The results showed that the removal rate of nitrogen and phosphorus by Chlorella microchlorella (33.01%) was higher than that by Chlorella vulgaris (25.4%). At the same time, the growth rate of Chlorella microphylla in sewage was faster than that of Chlorella microphylla. The removal efficiency of nitrogen and phosphorus and the growth of microalgae by (FN), immobilized (IN), (IN), gel ball (BB) were compared. The results showed that the removal rate of nitrogen and phosphorus by immobilized Chlorella microphylla (60.4%) was significantly higher than that by free Chlorella microphylla (76.6%). Phosphorus removal rate (30.5%) and sodium alginate entrapment fixation did not cause unrecoverable damage to Chlorella microphylla. Once the environment adapts, it will quickly restore its activity. A comparative experiment on nitrogen and phosphorus removal by immobilized Bacillus microphylla (IBN), immobilized Bacillus microphylla (IN), showed that the nitrogen and phosphorus removal efficiency of immobilized Bacillus sp. (IB) was significantly higher than that of immobilized Chlorella microchlosa (49.5%, 76.4%) and the highest nitrogen and phosphorus removal efficiency (60.488%) was higher than that of immobilized Chlorophyta microchlorella (49.5%, 76.4%), respectively. The removal rate of nitrogen and phosphorus by Bacillus orientalis was 31.730%. The internal environment of the immobilized bacteria and algae was beneficial to the growth of Chlorella microphylla. When pH reached the highest point, the mixed fixed pH was the most stable, and all the changes were within the optimum pH range of Chlorella microphylla. The results showed that the removal rate of nitrogen and phosphorus was the best at 1:1 when algal bacteria were fixed with 1: 1: 3: 2: 2: 3, respectively, and the removal rate of phosphorus was (60.4%). The activity of bacteria and algae was the highest when compared with the other side, and the removal efficiency of nitrogen and phosphorus was the best.
【學(xué)位授予單位】：燕山大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X703

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本文編號：2140273