植物-SBR系統(tǒng)污染物去除特性及鐵載體誘導表達研究
發(fā)布時間：2018-07-18 11:40
【摘要】：作為植物-活性污泥復合系統(tǒng)的主要功能單元,活性污泥、根際污泥及水生植物對復合系統(tǒng)處理污染物質具有重要作用。目前,對各功能單元在復合系統(tǒng)中的去污權重仍存爭議。本研究利用碳、氮穩(wěn)定同位素示蹤技術,精確分析植物-活性污泥復合系統(tǒng)中各功能單元對有機物和氨氮的吸收與去除權重,以及植物各部分的吸收去除活性。此外,還對復合系統(tǒng)在限鐵環(huán)境下鐵載體分泌特性進行了考察。復合系統(tǒng)中植物(美人蕉和風車草)對進水有機物沒有直接吸收去除作用。在兩種植物構建的復合系統(tǒng)中,活性污泥與根際污泥對有機物的去除比相差不大。在美人蕉-活性污泥復合系統(tǒng)中,活性污泥與根際污泥對有機物的去除分別為75.00%和25.00%,而在風車草-活性污泥復合系統(tǒng)中,分別為76.90%和23.10%�；钚晕勰嗯c根基污泥對有機物同化活性分別約為8.100 mg C/(g TOC·d)和7.600 mg C/(g TOC·d)。對氨氮的去除中,水生植物本身發(fā)揮了一定作用。美人蕉對進水氨氮的吸收去除權重為4.80%,高于風車草的3.23%。在美人蕉-活性污泥復合系統(tǒng)中,活性污泥與根際污泥對氨氮的去除權重分別為77.60%和17.60%,而在風車草-活性污泥復合系統(tǒng)中,分別為76.90%和19.80%。不同植物同化吸收的氨氮在根、莖、葉中的分配有所差異,其比重在美人蕉中分別為12.50%、50.00%和37.50%,在風車草中分別為14.80%、69.40%和15.80%。在同化吸收活性方面,美人蕉與風車草植物的氨氮同化活性分別為0.080 g N/(g TN·d)和0.004 g N/(g TN·d)。兩種植物均表現(xiàn)出,根系活性最高,莖部次之,葉部最低。在同位素示蹤實驗階段活性污泥與根際污泥微生物群落結構穩(wěn)定,主要菌群包括紅環(huán)菌科、叢毛單胞菌科、黃單胞菌屬、浮霉狀菌科、鞘脂h菌目、硝化螺旋菌科和伯克氏菌目等。根際微生物與活性污泥微生物群落有顯著差異。限鐵條件誘導鐵載體表達研究結果表明,兒茶酚型鐵載體和氧肟羧酸鹽型鐵載體是復合系統(tǒng)在限鐵環(huán)境下的主導攝取機制。分泌鐵載體的總鐵臨界值為0.020 mg/L~0.035 mg/L,出水鐵載體活性當量為9.9μM。通過對鐵載體的富集濃縮和類型檢測,測定其為兒茶酚型鐵載體和氧肟羧酸鹽型鐵載體。通過超高壓液相色譜-飛行時間質譜儀,初步檢測出Heterobactin B,Anacheline-H,2,3-二羥基蘇氨酸,Dibenarthin,Divanchrobactin,Dichrysobactin,弧菌載鐵素和副球菌素等兒茶酚型鐵載體,以及2-N-methylcoprogen B,Aerobactin,Arthrobactin,Fusarinine A,Dimethylcoprogen,Exochelin MN,Fusarinine B,Heterobactin B,Rhizobactin 1021,Synechobactin A,Synechobactin B和Thiazostatin等氧肟羧酸鹽型鐵載體。限鐵過程中系統(tǒng)污染物去除率有所降低,但仍具有較好的去除效果,其對COD的去除率高于70.00%,NH3-N去除率不低于90.00%。在限鐵條件下,系統(tǒng)微生物菌群結構發(fā)生變化,其中叢毛單胞菌科豐度明顯高于其他菌群,放線菌科豐度逐漸增大。通過對復合系統(tǒng)各功能單元的去污權重的研究,為復合系統(tǒng)的建模,工藝穩(wěn)定運行及制定管理方法提供數(shù)據(jù)支持。通過對復合系統(tǒng)在限鐵條件下鐵載體分泌特性的研究,探索了提高污水處理廠附加值的途徑,并有助于開發(fā)基于鐵載體絡合作用的重金屬同步去除工藝。
[Abstract]:As the main functional unit of the plant activated sludge complex system, activated sludge, rhizosphere sludge and aquatic plants have an important role in the treatment of contaminants in the compound system. At present, the decontamination weight of each functional unit in the compound system is still controversial. The absorption and removal weight of organic matter and ammonia nitrogen in the sludge compound system and the absorption and removal activity of various parts of the plant were also investigated. In addition, the secretory characteristics of the iron carrier in the complex system were also investigated. The plant (Canna and windmill grass) did not absorb the influent organic matter directly. In the compound system constructed by two plants, the removal ratio of organic matter to the activated sludge and the rhizosphere sludge is not significant. In the Canna activated sludge complex system, the removal of organic matter by activated sludge and Rhizosphere sludge is 75% and 25% respectively, while in the windmill grass active sludge complex system, 76.90% and 23.10%. activated sludge respectively. The assimilation activity of the organic matter to the organic matter was about 8.100 mg C/ (g TOC. D) and 7.600 mg C/ (g TOC d) respectively. In the removal of ammonia nitrogen, the aquatic plant itself played a certain role. The absorption of Canna to ammonia nitrogen absorption was 4.80%, higher than the 3.23%. of the windmill grass in the American banana activated sludge complex system, activated sludge and Rhizosphere The removal weight of ammonia nitrogen was 77.60% and 17.60% respectively. In the windmill grass active sludge composite system, the distribution of ammonia nitrogen in the roots, stems and leaves of different plants of 76.90% and 19.80%., respectively, were 12.50%, 50% and 37.50% in Canna, respectively, and 14.80%, 69.40% and 15.80%. in the windmill grass, respectively. In the assimilation activity, the ammonia nitrogen assimilation activity of Canna and windmill plant were 0.080 g N/ (g TN. D) and 0.004 g N/ (g TN d) respectively. The two plants showed the highest root activity, Be Jinno, the lowest leaf. The microbial community structure of the activated sludge and the rhizosphere sludge was stable and the main bacterial group was in the isotope tracer experiment stage. There are significant differences between the rhizosphere microorganisms and the microbial community of activated sludge. The results of iron carrier induced iron carrier and oxime carboxylate iron carrier are complex lines. The dominant uptake mechanism under iron limiting environment is that the total iron critical value of the secretory iron carrier is 0.020 mg/L~0.035 mg/L, and the activity equivalent of the effluent iron carrier is 9.9 M. through enrichment and concentration and type detection of the iron carrier, and the catechol iron carrier and oxime carboxylate type iron carrier are determined by the UHP liquid chromatography - time of flight mass spectrometry Heterobactin B, Anacheline-H, 2,3- two hydroxyl threonine, Dibenarthin, Divanchrobactin, Dichrysobactin, Vibrio and paramorotin, as well as 2-N-methylcoprogen B, Aerobactin, Arthrobactin, Fusarinine. In 1021, Synechobactin A, Synechobactin B and Thiazostatin and other oxime carboxylate type iron carriers. The removal efficiency of system pollutants is reduced in the process of iron limiting, but it still has a better removal effect. The removal rate of COD is higher than 70%. The removal rate of NH3-N is not less than 90.00%. under iron limiting conditions, and the structure of microbial flora changes in the system. The abundance of actinomycaceae was significantly higher than that of other flora, and the abundances of actinomycaceae increased gradually. Through the study of the decontamination weight of the functional units of the composite system, the data support for the modeling of the composite system, the stable operation of the process and the management methods were provided. The way to increase the added value of sewage treatment plants is explored, and it helps to develop the simultaneous removal process of heavy metals based on the cooperation of iron carriers.
【學位授予單位】：哈爾濱工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X703;X173
[Abstract]:As the main functional unit of the plant activated sludge complex system, activated sludge, rhizosphere sludge and aquatic plants have an important role in the treatment of contaminants in the compound system. At present, the decontamination weight of each functional unit in the compound system is still controversial. The absorption and removal weight of organic matter and ammonia nitrogen in the sludge compound system and the absorption and removal activity of various parts of the plant were also investigated. In addition, the secretory characteristics of the iron carrier in the complex system were also investigated. The plant (Canna and windmill grass) did not absorb the influent organic matter directly. In the compound system constructed by two plants, the removal ratio of organic matter to the activated sludge and the rhizosphere sludge is not significant. In the Canna activated sludge complex system, the removal of organic matter by activated sludge and Rhizosphere sludge is 75% and 25% respectively, while in the windmill grass active sludge complex system, 76.90% and 23.10%. activated sludge respectively. The assimilation activity of the organic matter to the organic matter was about 8.100 mg C/ (g TOC. D) and 7.600 mg C/ (g TOC d) respectively. In the removal of ammonia nitrogen, the aquatic plant itself played a certain role. The absorption of Canna to ammonia nitrogen absorption was 4.80%, higher than the 3.23%. of the windmill grass in the American banana activated sludge complex system, activated sludge and Rhizosphere The removal weight of ammonia nitrogen was 77.60% and 17.60% respectively. In the windmill grass active sludge composite system, the distribution of ammonia nitrogen in the roots, stems and leaves of different plants of 76.90% and 19.80%., respectively, were 12.50%, 50% and 37.50% in Canna, respectively, and 14.80%, 69.40% and 15.80%. in the windmill grass, respectively. In the assimilation activity, the ammonia nitrogen assimilation activity of Canna and windmill plant were 0.080 g N/ (g TN. D) and 0.004 g N/ (g TN d) respectively. The two plants showed the highest root activity, Be Jinno, the lowest leaf. The microbial community structure of the activated sludge and the rhizosphere sludge was stable and the main bacterial group was in the isotope tracer experiment stage. There are significant differences between the rhizosphere microorganisms and the microbial community of activated sludge. The results of iron carrier induced iron carrier and oxime carboxylate iron carrier are complex lines. The dominant uptake mechanism under iron limiting environment is that the total iron critical value of the secretory iron carrier is 0.020 mg/L~0.035 mg/L, and the activity equivalent of the effluent iron carrier is 9.9 M. through enrichment and concentration and type detection of the iron carrier, and the catechol iron carrier and oxime carboxylate type iron carrier are determined by the UHP liquid chromatography - time of flight mass spectrometry Heterobactin B, Anacheline-H, 2,3- two hydroxyl threonine, Dibenarthin, Divanchrobactin, Dichrysobactin, Vibrio and paramorotin, as well as 2-N-methylcoprogen B, Aerobactin, Arthrobactin, Fusarinine. In 1021, Synechobactin A, Synechobactin B and Thiazostatin and other oxime carboxylate type iron carriers. The removal efficiency of system pollutants is reduced in the process of iron limiting, but it still has a better removal effect. The removal rate of COD is higher than 70%. The removal rate of NH3-N is not less than 90.00%. under iron limiting conditions, and the structure of microbial flora changes in the system. The abundance of actinomycaceae was significantly higher than that of other flora, and the abundances of actinomycaceae increased gradually. Through the study of the decontamination weight of the functional units of the composite system, the data support for the modeling of the composite system, the stable operation of the process and the management methods were provided. The way to increase the added value of sewage treatment plants is explored, and it helps to develop the simultaneous removal process of heavy metals based on the cooperation of iron carriers.
【學位授予單位】：哈爾濱工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X703;X173
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