本文選題：2 + 4��； 參考：《湘潭大學(xué)》2015年碩士論文

【摘要】：由于CPs(氯酚類化合物)物質(zhì)的大規(guī)模生產(chǎn)和應(yīng)用,其排放量較大,已經(jīng)對自然環(huán)境和人類健康構(gòu)成了嚴(yán)重威脅。本研究以典型CPs物質(zhì)2,4,6-三氯酚(2,4,6-TCP)為研究對象,從湖南岳陽造紙廠厭氧反應(yīng)器中取得厭氧污泥,建立厭氧生物反應(yīng)體系,培養(yǎng)馴化具有降解2,4,6-TCP能力的厭氧顆粒污泥,考察馴化前后污泥微生物種群結(jié)構(gòu)的變化情況;在馴化成功后,采用間歇搖瓶試驗和PCR-DGGE(聚合酶鏈?zhǔn)椒磻?yīng)-變形梯度凝膠電泳)技術(shù),考察外加碳源和金屬對厭氧顆粒污泥降解2,4,6-TCP的效能及微生物種群結(jié)構(gòu)的影響;并且進(jìn)一步研究了葡萄糖濃度對厭氧顆粒污泥降解2,4,6-TCP的影響及其機(jī)理。研究得到了如下結(jié)論:(1)經(jīng)過2,4,6-TCP馴化之后,厭氧顆粒污泥的微生物多樣性減少,富集了6種2,4,6-TCP降解菌,分別為:Thermotog(熱袍菌屬)、Petrimonas sp.(理研菌屬)、Escherichia(埃希氏菌屬)、Bacillus(芽孢桿菌屬)、Clostridium(梭狀芽胞桿菌屬)、Burkholderia(伯克霍爾德氏菌屬);產(chǎn)生了新的2,4,6-TCP降解菌Shigella(志賀氏菌屬)。(2)不同種類的外加金屬對厭氧顆粒污泥降解2,4,6-三氯酚的效果影響差異較大,外加金屬鐵對其有促進(jìn)作用,可促進(jìn)Escherichia和Clostridium的生長;鎳和錳有抑制作用,Ni可促進(jìn)Acidobacterium(酸桿菌屬)的生長,Mn可促進(jìn)Mycobacterium(分支桿菌屬)的生長,同時Ni和Mn對Escherichia和Clostridium的生長都有抑制作用。(3)2,4,6-TCP的厭氧降解過程是一個共代謝過程。外加碳源的存在能有效的提高厭氧顆粒污泥對2,4,6-TCP的降解速率,不同碳源對2,4,6-TCP的厭氧降解促進(jìn)作用大小順序依次為:葡萄糖乙酸鈉丙酮酸鈉酵母膏。(4)葡萄糖濃度對厭氧顆粒污泥降解2,4,6-TCP的種群結(jié)構(gòu)有較大影響,當(dāng)葡萄糖濃度為2g/L時,2,4,6-TCP降解菌(Enterobucteriaceae bacterium.、Escherichia sp.、Shigella、Comamonas sp.、Clostridium)在整個微生物體系中占優(yōu)勢地位,且2,4,6-TCP降解率達(dá)到最大值,但葡萄糖濃度太高(如10g/L時),利用葡萄糖、但不能降解2,4,6-TCP的菌(Bacteroides)大量繁殖,2,4,6-TCP降解率下降。(5)在“厭氧顆粒污泥-2g/L葡萄糖”體系中,2,4,6-TCP的降解途徑為:2,4,6-TCP→2,4-DCP→4-CP→苯酚→礦化,“厭氧顆粒污泥-10g/L葡萄糖”體系中,2,4,6-TCP的降解路徑為:2,4,6-TCP→2,4-DCP→4-CP。
[Abstract]:Because of the large-scale production and application of CPs (chlorophenol compounds), their emissions are large, which has posed a serious threat to the natural environment and human health. In this study, anaerobic sludge was obtained from the anaerobic reactor of Yueyang Paper Mill in Hunan Province, and the anaerobic granular sludge with the ability of degrading 2n46-TCP was cultivated and domesticated. After acclimation, batch shaking flask test and PCR-DGGE (Polymerase chain reaction-deformation gradient Gel Electrophoresis) were used to investigate the changes of microbial population before and after acclimation. The effects of added carbon and metals on the degradation of 2n46-TCP and the microbial population structure of anaerobic granular sludge were investigated, and the effect of glucose concentration on the degradation of 2O46-TCP by anaerobic granular sludge and its mechanism were further studied. The results showed that the microbial diversity of anaerobic granular sludge decreased after the acclimation of 2N 4N 6-TCP, and 6 species of 2C46-TCP biodegradable bacteria were enriched, which were: 1. Thermotog (Pyropaea sp. Petrimonas). (Escherichia coli) Bacillus (Bacillus sp.) Clostridium (Clostridium sp.) (Bacillus sp.); A new strain of Shilagelia (Shilagelia. 2) produced a new strain of Shilagelia (Shilagelia. 2). Different kinds of added metals degrade the anaerobic granular sludge of 2H4. 6 The effect of trichlorophenol was different. The addition of metal iron can promote the growth of Escherichia and Clostridium, and Ni and mn can inhibit the growth of Acidobacterium. Mn can promote the growth of Mycobacterium. At the same time, both Ni and mn inhibited the growth of Escherichia and Clostridium. The anaerobic degradation process of 46-TCP was a co-metabolic process. The existence of additional carbon source can effectively improve the degradation rate of 2N 46-TCP by anaerobic granular sludge. The order of promoting anaerobic degradation by different carbon sources was as follows: sodium glycolate sodium pyruvate yeast extract. 4) glucose concentration had a great effect on the population structure of anaerobic granular sludge degradation of 2H4 + 6-TCP. When glucose concentration is 2 g / L, the bacteria Enterobucteriaceae bacterium Escherichia sp. Shigellaus Comamonas sp. Clostridium is dominant in the whole microbial system, and the degradation rate of 2G / L is the highest, but the concentration of glucose is too high (for example, 10 g / L). However, Bacteroides, a bacterium that can not degrade 2G / 4G / L TCP, propagated in large numbers. The degradation rate of 2G / 4N / 6TCP was decreased. 5) in the anaerobic granular sludge-2g / L glucose system, the degradation pathway was: 1 / 2G / L / L glucose / 2g / L / L / g / L glucose / glucose system, respectively, and the pathway of degradation was as follows: 1 / 2N / 4 / 6-TCP / 24-DCP / 4-DCP / 4-CP phenol mineralization. In the anaerobic granular sludge -10g / L glucose system, the degradation path of 46-TCP is: 1 / 2G / L / L Glucose-10 g / L glucose.
【學(xué)位授予單位】：湘潭大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X703;X172

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

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本文編號：2032743