本文選題：馴化 + 厭氧消化　； 參考：《環(huán)境科學(xué)學(xué)報(bào)》2016年12期

【摘要】：為研究馴化對(duì)餐廚垃圾厭氧消化系統(tǒng)微生物群落結(jié)構(gòu)的影響,在單相完全攪拌式(CSTR)反應(yīng)器內(nèi),以農(nóng)村戶用沼氣池污泥為接種污泥,進(jìn)行了餐廚垃圾中溫厭氧消化.反應(yīng)器在3 g·L-1·d-1(以VS計(jì))的負(fù)荷下成功啟動(dòng),并連續(xù)45 d維持性能穩(wěn)定,表明馴化成功.期間采用454焦磷酸測序技術(shù)分析了馴化前后系統(tǒng)內(nèi)的微生物群落結(jié)構(gòu).結(jié)果表明,微生物群落結(jié)構(gòu)與底物密切相關(guān),馴化后細(xì)菌及古菌群落都發(fā)生明顯變化.從細(xì)菌群落看,與復(fù)雜有機(jī)物降解相關(guān)的菌類顯著下降(如梭菌綱(Clostridia)和(vadin HA17),而易降解碳水化合物發(fā)酵菌(如Petrimonas)和脂肪降解菌(如Erysipelotrichia)顯著增加.這與餐廚垃圾易降解有機(jī)物含量高,且富含淀粉和脂肪相關(guān).豐富的易降解有機(jī)物還使得反應(yīng)器內(nèi)總揮發(fā)性脂肪酸(VFA)濃度((2203±174)mg·L-1)遠(yuǎn)高于種泥水平((222.0±0.3)mg·L-1),這導(dǎo)致了產(chǎn)甲烷菌由乙酸型的甲烷鬃菌屬(Methanosaeta,占85.01%)絕對(duì)主導(dǎo)轉(zhuǎn)向氫營養(yǎng)型的甲烷螺菌屬(Methanospirillum,占35.35%)、甲烷囊菌屬(Methanoculleus,占9.89%)與之(46.97%)共同主導(dǎo)的局面.然而,馴化后Methanosaeta在非最優(yōu)條件下依然保持主導(dǎo)地位,可見接種污泥的群落組成對(duì)厭氧消化系統(tǒng)群落結(jié)構(gòu)的塑造也具有重大影響.
[Abstract]:In order to study the effect of domestication on the microbial community structure in the anaerobic digestion system of food waste, the anaerobic digestion of kitchen waste was carried out in a single phase complete stirred (CSTR) reactor with the sludge of the rural household biogas pool as inoculated sludge. The reactor was successfully started under the load of 3 G. L-1. D-1 (VS), and the performance was stable continuously. During the period of domestication, the microbial community structure in the system was analyzed by 454 pyrosequencing technology. The results showed that the microbial community structure was closely related to the substrate, and the bacteria and the Archaea community were obviously changed after the acclimatization. From the bacterial community, the bacteria related to the degradation of complex organic matter were significantly decreased (such as spindle bacteria. The class (Clostridia) and (vadin HA17), while the easily degraded carbohydrate fermentor (such as Petrimonas) and adipose degrading bacteria (such as Erysipelotrichia) have increased significantly. This is associated with high content of biodegradable organic matter in kitchen waste and rich in starch and fat. The rich and easily degraded organic compounds also make the total volatile fatty acid (VFA) concentration in the reactor (2203 +. 174) mg. L-1) is much higher than the level of mud ((222 + 0.3) mg. L-1), which leads to the absolute dominance of methanogenic bacteria of methanogenic bacteria (Methanosaeta, 85.01%) to the hydrogen nutritive methanomyces (Methanospirillum, 35.35%), methanomyces (Methanoculleus, 9.89%) and (46.97%) Co dominant. However, domestication is domesticated. The post Methanosaeta remains dominant under the non optimal conditions, and the community composition of the inoculated sludge has a significant impact on the construction of the community structure of the anaerobic digestion system.
【作者單位】： 重慶大學(xué)三峽庫區(qū)生態(tài)環(huán)境教育部重點(diǎn)試驗(yàn)室;
【基金】："十一五"國家科技支撐計(jì)劃重點(diǎn)項(xiàng)目(No.2010BAC67B01) 重慶市科技計(jì)劃重點(diǎn)項(xiàng)目~~
【分類號(hào)】：X799;X172

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張旭,王寶貞,朱宏;厭氧消化體系的多相化學(xué)平衡[J];中國沼氣;1997年03期

2 彭武厚;李惠;陳幸;;利福霉素菌體的厭氧消化[J];生化通訊;1980年05期

3 竺強(qiáng);金霉素廢水厭氧消化初步研究[J];中國沼氣;1997年01期

4 史宏偉;鄒德勛;左劍惡;劉研萍;朱保寧;李秀金;毛菁菁;;梯度負(fù)荷下果蔬垃圾厭氧消化性能及微生物群落結(jié)構(gòu)的研究[J];環(huán)境科學(xué)學(xué)報(bào);2012年01期

5 趙慶良，王寶貞，Ｇ．庫格爾;厭氧消化中的重要中間產(chǎn)物──有機(jī)酸[J];哈爾濱建筑大學(xué)學(xué)報(bào);1996年05期

6 蘇建文;王彩東;王俊超;徐尚營;;克拉維酸鉀菌渣中溫單級(jí)厭氧消化實(shí)驗(yàn)研究[J];甘肅聯(lián)合大學(xué)學(xué)報(bào)(自然科學(xué)版);2013年01期

7 史宏偉;鄒德勛;左劍惡;朱保寧;劉研萍;李秀金;;原料差異對(duì)厭氧消化微生物群落的影響[J];農(nóng)業(yè)環(huán)境科學(xué)學(xué)報(bào);2011年08期

8 寧東;臺(tái)明青;馮果;;聚丙烯酰胺影響共厭氧消化污泥脫水性能研究[J];環(huán)境污染與防治;2011年02期

9 郭養(yǎng)浩，，陳金志，潘文良，洪曉峰，曾兆勇;啤酒廢水二相厭氧消化動(dòng)力學(xué)研究[J];微生物學(xué)通報(bào);1995年05期

10 ;環(huán)境保護(hù)上的應(yīng)用[J];生物技術(shù)通報(bào);1989年09期

相關(guān)碩士學(xué)位論文 前1條

1 梁銀春;有機(jī)固體廢棄物厭氧、好氧發(fā)酵特性及細(xì)菌多樣性對(duì)比研究[D];廣西大學(xué);2015年

本文編號(hào)：1912116