本文選題：汞 + 砷��； 參考：《生態(tài)毒理學報》2016年06期

【摘要】：為深入探討汞(Hg)和砷(As)對水生生物的慢性毒性效應,以日本沼蝦(Macrobrachium nipponense)為受試生物,以超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、金屬硫蛋白(MT)和丙二醛(MDA)為測試分子標志物,研究上覆水中不同濃度Hg和As單一及聯(lián)合暴露對日本沼蝦肝胰腺的氧化損傷及聯(lián)合作用機制。結果表明:Hg和As單一及聯(lián)合暴露對4種分子標志物均可產(chǎn)生不同程度的抑制或激活效應。其中,SOD活性均受到抑制,且高濃度As(1000μg·L-1)暴露10 d的抑制率最大,高達59.13%;而CAT活性整體被激活,Hg單獨暴露下的激活效應要高于As,且在聯(lián)合暴露組中隨著暴露時間的增加呈現(xiàn)出顯著的劑量效應關系(P0.05);MT和MDA含量在Hg和As的暴露下均顯著增加(P0.05)。其中MT對Hg的螯合能力大于As,As單獨暴露3 d后MDA變化較小,但10 d后其含量顯著增加(P0.05)。析因方差分析發(fā)現(xiàn),Hg-As對機體的聯(lián)合作用機制主要表現(xiàn)為協(xié)同作用。綜合生物標志物響應指數(shù)(IBR)評價證實,聯(lián)合毒性要高于單獨毒性,Hg與As在高濃度下聯(lián)合暴露10 d的IBR值最大,毒性最強,高濃度As單獨暴露處理組的毒性次之;同時,機體自身隨暴露時間延長對Hg也表現(xiàn)出一定的解毒功能。
[Abstract]:In order to investigate the chronic toxicity of HG and As3) to aquatic organisms, Macrobrachium nipponensewas used as the tested organism, and superoxide dismutase (SOD), catalase (CATN), metallothionein (MTT) and malondialdehyde (MDA) were used as molecular markers. The oxidative damage to hepatopancreas of Macrobrachium japonicus by single and combined exposure of Hg and as in overlying water was studied. The results showed that the single and combined exposure of as and Hg could inhibit or activate the four molecular markers in different degrees. The activity of sod was inhibited, and the inhibitory rate of high concentration of As(1000 渭 g L ~ (-1) was the highest in 10 days. The activation effect of CAT activity was higher than that of As. in the combined exposure group, there was a significant dose-effect relationship between P0.05MMT and MDA contents in the combined exposure group. The contents of P0.05MT and MDA increased significantly under the exposure of Hg and as, and the activation effect was higher than that of As-HG alone, and the concentration of P0.05T and MDA increased significantly with the increase of exposure time in the combined exposure group. The chelating ability of MT to Hg was higher than that of Asmas alone for 3 days, but the content of MDA increased significantly after 10 days of exposure. The analysis of variance showed that the mechanism of Hg-As combined action was mainly synergistic. The comprehensive biomarker response index (IBR) evaluation confirmed that the combined toxicity was higher than the IBR value of combined exposure of as and Hg alone for 10 days, the toxicity was the highest, and the toxicity of as alone exposed group was the second, at the same time, there was no significant difference between the two groups. With the prolongation of exposure time, the organism also showed certain detoxification function.
【作者單位】： 南京水利科學研究院生態(tài)環(huán)境研究中心;河海大學環(huán)境學院;江蘇省水文水資源勘測局;
【基金】：江蘇省水利科技重大項目(2015005) “十二五”水專項-“淮河流域水質-水量-水生態(tài)聯(lián)合調度關鍵技術研究與示范”課題(No.2014ZX07204-006-01) 公益性行業(yè)科研專項經(jīng)費項目—“淮河流域防洪工程體系多目標協(xié)同調控研究”課題(201501007)
【分類號】：X171.5

【參考文獻】

相關期刊論文 前10條

1 韓雨薇;張彥峰;陳萌;鐘文玨;祝凌燕;;沉積物中重金屬Pb和Cd對河蜆的毒性效應研究[J];生態(tài)毒理學報;2015年04期

2 彭雪;黃曉亮;陳思涵;陳上靜;應雪萍;;鎘、汞單獨及聯(lián)合脅迫對中華絨螯蟹的急性毒性[J];水產(chǎn)科學;2015年04期

3 李磊;蔣玫;沈新強;王云龍;吳慶元;�？∠�;許高鵬;;Cr(VI)對脊尾白蝦(Exopalaemon carinicauda)幼蝦暴露和恢復期肝胰臟的SOD活性、MDA及MTs含量的影響[J];生態(tài)毒理學報;2014年06期

4 李詩逸;孫繼鵬;易瑞灶;洪專;陳發(fā)河;;金屬離子對水生動物體內金屬硫蛋白應激誘導研究進展[J];水產(chǎn)科學;2014年09期

5 王輝;謝鑫源;;Cd、Cu和Pb復合污染對蚯蚓抗氧化酶活性的影響[J];環(huán)境科學;2014年07期

6 趙漢取;施沁璇;沈萍萍;韋肖杭;朱俊杰;;低濃度Cd~(2+)脅迫對青魚組織SOD活性和MT誘導的影響[J];水生態(tài)學雜志;2014年02期

7 王偉莉;閆振廣;何麗;王曉南;孟雙雙;鄭欣;劉征濤;;五種底棲動物對優(yōu)控污染物的敏感性評價[J];中國環(huán)境科學;2013年10期

8 畢春娟;陳振樓;李猛;張晶晶;孫瑋瑋;;再懸浮作用下長江口近岸沉積物中Cd、Pb和Cr的遷移與釋放[J];環(huán)境科學;2011年09期

9 王學鋒;陳海剛;蔡文貴;秦潔芳;賈曉平;;汞離子脅迫對紅鰭笛鯛抗氧化酶及乙酰膽堿酯酶活性的影響[J];水產(chǎn)學報;2010年12期

10 許燕;楊潔;孫靜秋;俞亞東;葉軍強;;凡納濱對蝦不同組織內SOD、POD酶的細胞化學定位[J];水生生物學報;2010年02期

【共引文獻】

相關期刊論文 前10條

1 陽金希;張彥峰;祝凌燕;;中國七大水系沉積物中典型重金屬生態(tài)風險評估[J];環(huán)境科學研究;2017年03期

2 解偉;郭冉;夏輝;王小瑞;李趙嘉;李W歐，

本文編號：1850158