本文選題：生物脫硫 + 二苯并噻吩��； 參考：《中國石油大學(xué)(華東)》2015年碩士論文

【摘要】：二苯并噻吩及其衍生物是高硫原油的重要組成,它的存在不僅降低了原油品質(zhì),而且燃燒時排放二氧化硫,二氧化硫進(jìn)入到外環(huán)境會造成環(huán)境質(zhì)量的嚴(yán)重下降,并潛在威脅著生態(tài)系統(tǒng)及人體健康。本文以二苯并噻吩(DBT)作為模型化合物,從長期被石油污染的土壤中分離出專一性脫硫菌AS-21,采用16SrDNA序列法對菌株進(jìn)行了鑒定,并對AS-21的培養(yǎng)條件和脫硫條件進(jìn)行了優(yōu)化;在此基礎(chǔ)上,將AS-21接種到原油采出液中,考察該AS-21的脫硫效果;最后將AS-21制成休止細(xì)胞,考察不同濃度休止細(xì)胞的脫硫效果。本論文從不同油田區(qū)采集受污染的土樣,進(jìn)行初篩、復(fù)篩、分離純化,得到兩株脫硫菌AS-21和AS-26。該兩株菌培養(yǎng)三天后,脫硫率分別達(dá)到73%和69%;脫硫菌AS-21為革蘭氏陰性菌,且菌體為短桿形,對該菌株進(jìn)行DNA提取,并經(jīng)PCR擴(kuò)增后測序,利用Blast程序?qū)⒃摼?6SrDNA序列分析結(jié)果與GenBank中數(shù)據(jù)進(jìn)行比對,比對結(jié)果顯示該AS-21脫硫菌與紅假單胞菌屬Rhodopseudomonas 16SrDNA序列同源性最高,達(dá)到99%,故將AS-21初步鑒定為紅假單胞菌屬;通過對單因素影響研究和分析,得到菌株AS-21的最優(yōu)培養(yǎng)條件和脫硫條件:培養(yǎng)溫度為30℃,培養(yǎng)基初始pH為7.5,碳源為10g/L的甘油,氮源為2g/L的氯化銨,初始DBT濃度為100mg/L;通過響應(yīng)曲面法研究確定生長溫度為29.70℃,pH為7.43,DBT濃度為105.47mg/L時可得到最大脫硫率74%及最大菌株生長量2.49g/L;AS-21對采出液中所投放的DBT的脫硫?qū)嶒灲Y(jié)果表明,當(dāng)向采出液中加入一系列營養(yǎng)物質(zhì)后,脫硫率最高可為18.38%,由AS-21的生長曲線可知,0~15h為AS-21的生長停滯期,15~50h為AS-21的生長對數(shù)期,此后其生長進(jìn)入穩(wěn)定期,將對數(shù)末期的菌液制成休止細(xì)胞,對休止細(xì)胞脫硫效果進(jìn)行考察,其脫硫率可達(dá)81%,高于普通菌株。
[Abstract]:Dibenzothiophene and its derivatives are important components of high-sulfur crude oil. Its existence not only reduces the quality of crude oil, but also emits sulfur dioxide during combustion.And potential threats to ecosystems and human health.In this paper, using dibenzothiophene (DBT) as a model compound, AS-21 was isolated from soil contaminated by petroleum for a long time. The strain was identified by 16SrDNA sequence method, and the culture conditions and desulfurization conditions of AS-21 were optimized.On this basis, AS-21 was inoculated into crude oil recovery solution to investigate the desulphurization effect of AS-21, and AS-21 was made into repose cells to investigate the desulphurization effect of different concentrations of resting cells.In this paper, the contaminated soil samples were collected from different oil fields, screened, screened, separated and purified, and two desulphurization bacteria AS-21 and AS-26 were obtained.After three days of culture, the desulphurization rates of the two strains reached 73% and 69%, respectively, and the desulphurization bacteria AS-21 was gram-negative, and the strain was short rod. The DNA was extracted and sequenced by PCR amplification.The results of 16SrDNA sequence analysis of this bacterium were compared with the data in GenBank by Blast program. The results showed that the homology of 16SrDNA sequence between AS-21 and Rhodopseudomonas 16SrDNA was the highest, which reached 99%, so AS-21 was preliminarily identified as Rhodopomonas.The optimum culture conditions and desulphurization conditions of the strain AS-21 were obtained through the study and analysis of the single factor: the culture temperature was 30 鈩，

本文編號：1739872