本文關(guān)鍵詞： 反硝化型厭氧甲烷氧化菌 群落結(jié)構(gòu) 豐度 三峽庫區(qū) 消落帶濕地　出處：《武漢紡織大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：由“Candidatus Methylomirabilis oxyfera”(M.oxyfera)介導(dǎo)的反硝化型厭氧甲烷氧化(nitrate/nitrite dependent anaerobic methane oxidation,n-damo)過程發(fā)現(xiàn)于2006年,它的發(fā)現(xiàn)對(duì)完善全球碳氮循環(huán)理論有著重要意義。目前對(duì)n-damo的研究主要集中在不同類型生境和污水處理反應(yīng)器中,該過程在三峽庫區(qū)消落帶這一高擾動(dòng)生境中是否存在仍未知。本文利用分子生物學(xué)手段與理化性質(zhì)分析對(duì)三峽庫區(qū)消落帶中n-damo的存在、分布與多樣性進(jìn)行了研究,結(jié)果如下:(1)本研究通過定量PCR方法成功定量出三峽庫區(qū)消落帶濕地中n-damo菌的豐度,并采用基因克隆文庫技術(shù)在庫區(qū)消落帶中成功檢測(cè)出n-damo菌特異性序列,證實(shí)了n-damo菌在該大幅水位波動(dòng)背景下的消落帶濕地中的存在。(2)三峽庫區(qū)消落帶濕地中存在多個(gè)與M.oxyfera親緣關(guān)系較近的分支。在所研究的無人為干擾的自然消落帶濕地和受人為影響的消落帶濕地中,僅水下沉積物中檢測(cè)到屬Group a的n-damo菌,同時(shí)所選兩個(gè)消落帶濕地中的優(yōu)勢(shì)n-damo菌均屬Group b。(3)通過q PCR技術(shù)在兩個(gè)庫區(qū)消落帶濕地不同高程樣品中菌檢測(cè)到了一定數(shù)量的n-damo菌,豐度范圍為102~105 copies g-1干土。在庫區(qū)消落帶濕地沉積物中n-damo菌豐度大多明顯高于土壤樣品中n-damo菌豐度(2013年10月自然消落帶中沉積物、土壤樣品n-damo菌豐度變化范圍分別為1.05×103~1.45×103copies g-1干土和4.79×102~5.16×102 copies g-1干土,2014年4月則為4.21×104~5.32×104 copies g-1干土和3.17×103~7.69×103 copies g-1干土,2013年10月受人為干擾的消落帶濕地分別為2.01×103~3.54×103 copies g-1干土和8.98×102~1.09×102 copies g-1干土),表明水下沉積物是消落帶中n-damo菌的主要分布區(qū)域。相關(guān)性分析結(jié)果顯示,自然消落帶濕地中n-damo菌豐度與環(huán)境因子無明顯相關(guān)性,受人為影響的消落帶濕地中有機(jī)質(zhì)含量與n-damo菌群豐度相關(guān)性顯著。
[Abstract]:Denitrifying anaerobic methane Oxidation mediated by "Candidatus Methylomirabilis oxyfera" M. oxyfera. Nitrate/nitrite dependent anaerobic methane oxidation. The n-damo) process was discovered in 2006. The discovery of n-damo is of great significance to improve the global carbon and nitrogen cycle theory. At present, the research on n-damo is mainly focused on different types of habitats and sewage treatment reactors. It is still unknown whether this process exists in the highly disturbed habitat of the three Gorges reservoir area. In this paper, the existence of n-damo in the collapse zone of the three Gorges reservoir area is analyzed by molecular biological methods and physicochemical properties. The distribution and diversity were studied. The results were as follows: 1) in this study, the abundance of n-damo bacteria in the wetland of the three Gorges Reservoir area was determined by quantitative PCR method. The specific sequence of n-damo bacteria was successfully detected by gene cloning library technique in the banding zone of reservoir area. It is confirmed that n-damo bacteria exist in the wetland of the subsidence zone under the background of large water level fluctuation. There are many branches close to M. oxyfera in the wetland of the three Gorges Reservoir area. Only n-damo bacteria belonging to Group a were detected in underwater sediments. The dominant n-damo bacteria in the wetland of the two decaying zones were all Group B. A certain number of n-damo bacteria were detected by Q PCR technique in different elevation samples of wetland in two reservoir areas. The abundance of n-damo bacteria in wetland sediments of reservoir area is significantly higher than that in soil samples (P < 0.05). The abundance range of n-damo bacteria in dry soil is 102 ~ 10 ~ 5 copies g ~ (-1). In October 2013, sediments in the natural subsidence zone. The variation ranges of n-damo abundance in soil samples were 1.05 脳 10 ~ (3) C ~ (-1) and 4.79 脳 10 ~ (2) ~ (2) C _ (5.16) 脳 10 ~ (2), respectively. Copies g-1 dry soil. In April 2014, it was 4.21 脳 104 copies g-1 dry soil and 3.17 脳 10 3 copies g -1 dry soil and 3.17 脳 10 3 脳 10 4 copies g -1 dry soil and 3.17 脳 10 3 脳 10 3 copies g -1 dry soil. G-1 dry soil. In October 2013, the number of artificial disturbance wetlands was 2.01 脳 10 ~ 3 ~ 3.54 脳 10 ~ (3) copies, respectively. G-1 dry soil and 8.98 脳 102 copies g-1 dry soil. . The results of correlation analysis showed that there was no significant correlation between the abundance of n-damo bacteria and environmental factors. There was a significant correlation between the content of organic matter and the abundance of n-damo flora.
【學(xué)位授予單位】：武漢紡織大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X172

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳鮮艷;宋連春;郭占峰;高學(xué)杰;張強(qiáng);;長(zhǎng)江三峽庫區(qū)和上游氣候變化特點(diǎn)及其影響[J];長(zhǎng)江流域資源與環(huán)境;2013年11期

2 曹詩圖;;三峽地區(qū)的地質(zhì)地貌特征及其經(jīng)濟(jì)評(píng)價(jià)[J];水電科技進(jìn)展;1998年01期

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