本文關(guān)鍵詞：蚯蚓糞中除臭微生物的篩選及培養(yǎng)條件優(yōu)化　出處：《東北農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

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【摘要】：隨著工農(nóng)業(yè)的發(fā)展和人們對周圍環(huán)境要求的日益提高,惡臭污染越來越引起人們的廣泛關(guān)注。研究表明,惡臭污染中主要的污染物就是氨氣和硫化氫,會造成人體的慢性中毒,甚至威脅生命。蚯蚓糞是具有高孔隙度和比表面積的顆粒狀物質(zhì),具有吸附臭氣的作用,并且蚯蚓糞中含有大量微生物,可促進惡臭物質(zhì)的分解而達到除臭的目的。但是目前國內(nèi)有關(guān)于蚯蚓糞的微生物學(xué)研究進行的還不夠深入,惡臭處理的研究和應(yīng)用報道也很少。本試驗以蚯蚓處理城市污泥得到的蚯蚓糞為研究對象,利用蚯蚓糞具有除臭功能的特性,從中篩選高效除臭菌株,將得到的菌種進行復(fù)配,利用復(fù)合菌株進行惡臭降解能力測試,并進行復(fù)合菌株培養(yǎng)條件的優(yōu)化,得到以下結(jié)論:蚯蚓糞樣品的高通量測序結(jié)果表明,蚯蚓糞中含有除氨能力較好的產(chǎn)堿桿菌屬和脫硫能力較好的芽胞桿菌屬。利用初篩和復(fù)篩相結(jié)合的方法,經(jīng)過25天的培養(yǎng)從蚯蚓糞中分離出6株高效除氨菌NH1、NH4、NH6、NH9、NH11、NH12和NH13,5株脫硫菌HS2、HS3、HS5、HS7和HS10。除氨能力最強的菌種為NH1,NH3去除率為50.34%;脫硫能力最強的菌種為HS3,H2S去除率為54.69%。將復(fù)篩得到的除氨菌和脫硫菌分別進行菌種復(fù)配,利用復(fù)合菌種進行雞糞的惡臭降解試驗,結(jié)果表明:在培養(yǎng)25天時,NH1與NH9、NH11除氨復(fù)合菌系使NH3的釋放量與對照相比降低了58.21%;HS2與HS3、HS5脫硫復(fù)合菌系使H2S的釋放量與對照相比降低了72.55%。在相同的培養(yǎng)條件下,復(fù)合菌系的除臭效果均高于復(fù)篩選出的單個菌株的效果。對復(fù)合菌系中的6株菌分別做生理生化鑒定和分子生物學(xué)鑒定,結(jié)果表明:NH1為產(chǎn)堿桿菌屬(Alcaligenes sp.),NH9為檸檬酸桿菌屬(Citrobacter sp.),NH11為假單胞菌屬(Pseudomonas sp.);HS2為節(jié)桿菌屬(Arthrobacter sp.),HS3為嗜麥芽寡養(yǎng)單胞菌屬(Stenotrophomonas sp.),HS5為芽孢桿菌屬(Bacillus sp.)。對復(fù)合菌系進行混合培養(yǎng)條件優(yōu)化試驗,結(jié)果表明:除氨復(fù)合菌系最佳培養(yǎng)條件為溫度30℃,p H 7.5,搖床轉(zhuǎn)速175 rpm,接種量10%。脫硫復(fù)合菌系最佳培養(yǎng)條件為溫度28℃,p H 7.0,搖床轉(zhuǎn)速150 rpm,接種量15%。
[Abstract]:With the development of industry and agriculture and the increasing demands on the environment, the odor pollution has attracted more and more attention. The research shows that ammonia and hydrogen sulfide are the main pollutants in the odor pollution. Earthworm dung is a granular substance with high porosity and specific surface area, which can adsorb odor and contain a large number of microbes in earthworm dung. It can promote the decomposition of odors and achieve the purpose of deodorization. However, there are some microbiological studies on earthworm dung in China, which are not deep enough. The research and application of deodorant treatment were also few. In this experiment, earthworm dung from urban sludge treatment was used as the research object, and the effective deodorizing strain was screened by using earthworm manure with deodorization function. The compound strain was used to test the ability of odor degradation, and the culture conditions of the compound strain were optimized. The following conclusions were obtained: the high-throughput sequencing results of earthworm feces showed that: 1. The earthworm dung contains Alcaligenes with better ammonia removal ability and Bacillus with better desulphurization ability. The method of combination of primary screening and double screening is used. After 25 days of culture, 6 strains of high efficiency ammonia removal bacteria, NH 1, NH 4, NH 6, NH 6, NH 9, NH 11, NH 12, and NH 13, 5 strains of desulphurizing bacteria, HS2 + HS3, were isolated from earthworm dung. HS5 + HS7 and HS10. The highest ammonia removal rate was NH1 (NH3) removal rate (50.34). The removal rate of HS3H2S was 54.690.Ammonia-removing bacteria and desulphurizing bacteria were mixed separately, and the odor degradation test of chicken manure was carried out by using compound bacteria. The results showed that the release of NH3 was reduced by 58.21% compared with that of the control at 25 days after culture. The amount of H2S released by HS2 and HS3 / HS5 desulphurization composite strain was 72.55% lower than that of the control, under the same culture conditions. The deodorization effect of the compound strain was higher than that of the single strain screened again. The physiological and biochemical identification and molecular biological identification of 6 strains of the compound strain were done respectively. The results showed that the proportion of NH _ 1 to Alcaligenes sp. and NH _ 9 was Citrobacter sp.). NH11 is Pseudomonas sp. HS2 belongs to Arthrobacter sp. HS3, which belongs to Stenotrophomonas sp.). HS5 was Bacillus sp. The optimum culture condition of the compound strain was optimized. The results showed that the optimum culture condition of the compound strain was 30 鈩，

本文編號：1438959