本文關(guān)鍵詞：固定化酵母細(xì)胞載體制備及去除廢水中苯酚效果研究　出處：《西南交通大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 固定化 載體制備 酵母工程菌 苯酚 去除率

【摘要】：通過顯微鏡觀察海藻酸鈉、聚乙烯醇及海藻酸鈉和聚乙烯醇混合材料的表觀特征,確定了以海藻酸鈉和聚乙烯醇為包埋材料,制備固定化酵母菌和固定化酵母工程菌的方法。根據(jù)固定化材料包埋條件標(biāo)準(zhǔn)(傳質(zhì)性能、機(jī)械強(qiáng)度、降酚能力、重復(fù)使用性),確定了最優(yōu)包埋條件：聚乙烯醇5%、海藻酸鈉3%。以廢水中苯酚初始濃度、環(huán)境溫度以及廢水pH為控制因子,開展了大量不同環(huán)境條件下固定化酵母菌及酵母工程菌降解廢水中苯酚效果的試驗(yàn)研究。結(jié)果表明。固定化酵母工程菌隨著初始濃度、環(huán)境溫度以及pH值的增大,廢水中苯酚去除率均呈現(xiàn)先增大后減小的規(guī)律,這說明,對于各個(gè)控制因子,固定化酵母工程菌對于廢水中苯酚的去除均存在一個(gè)最大活性條件。基于試驗(yàn)結(jié)果分析,我們得出,固定化酵母工程菌在處理模擬廢水中苯酚去除率最快最大時(shí),pH為6,溫度為25℃,濃度為1800mg/L。并且模擬出最優(yōu)條件下,固定化酵母工程菌降解廢水中苯酚去除率隨時(shí)間呈指數(shù)規(guī)律增長。開展了不同苯酚初始濃度、不同環(huán)境溫度、以及不同廢水pH值條件下,固定化酵母工程菌和固定化酵母菌降去除水中苯酚的去除率的對比分析。得出結(jié)論,固定化酵母工程菌對于廢水中苯酚的去除率均大于相同條件下固定化酵母菌降解廢水中苯酚的去除率。并且當(dāng)條件發(fā)生變化時(shí),固定化酵母工程菌具有更強(qiáng)的適應(yīng)性。對苯酚降解動(dòng)力學(xué)進(jìn)行試驗(yàn),通過廢水中苯酚比去除率計(jì)算及分析,發(fā)現(xiàn)固定化酵母工程菌降解苯酚的過程符合Haldane模型�；贖aldane模型,通過擬合分析,得到了苯酚降解動(dòng)力學(xué)方程,并利用不同工況數(shù)據(jù)對動(dòng)力學(xué)方程的普遍適用性進(jìn)行了反演。以上研究結(jié)果對于廢水中苯酚去除的菌種選擇,以及固定化酵母工程菌實(shí)際應(yīng)用于廢水中苯酚去除的環(huán)境因子控制和不同去除時(shí)間后的廢水中殘留苯酚濃度的預(yù)測具有一定的參考意義。
[Abstract]:The apparent characteristics of sodium alginate, polyvinyl alcohol and the mixture of sodium alginate and polyvinyl alcohol were observed under microscope, and the embedding materials of sodium alginate and polyvinyl alcohol were determined. Methods of preparing immobilized yeast and immobilized yeast engineering bacteria. According to immobilized material embedding conditions (mass transfer property mechanical strength phenol reducing ability reusability). The optimal embedding conditions were determined as follows: polyvinyl alcohol 5 and sodium alginate 3. The initial concentration of phenol in wastewater, environmental temperature and pH of wastewater were taken as control factors. The effect of immobilized yeast and yeast engineering bacteria on the degradation of phenol in wastewater under different environmental conditions was studied. The results showed that the immobilized yeast engineering bacteria were treated with the initial concentration. The removal rate of phenol in wastewater increased first and then decreased with the increase of environmental temperature and pH value. There is a maximum activity condition for phenol removal from wastewater by immobilized yeast engineering bacteria. The best removal rate of phenol in immobilized yeast engineering bacteria was pH 6, temperature 25 鈩，

本文編號：1429437