鈾脅迫對(duì)反枝莧生理生化指標(biāo)的影響分析
發(fā)布時(shí)間:2018-01-12 16:26
本文關(guān)鍵詞:鈾脅迫對(duì)反枝莧生理生化指標(biāo)的影響分析 出處:《環(huán)境科學(xué)與技術(shù)》2016年11期 論文類型:期刊論文
更多相關(guān)文章: 鈾脅迫 反枝莧 植物修復(fù) 抗逆性
【摘要】:為探究鈾脅迫下富集植物反枝莧的抗逆機(jī)制及鈾對(duì)反枝莧的毒理效應(yīng),通過(guò)盆栽控制性試驗(yàn),研究了土壤不同濃度鈾(U)脅迫(0、25、50、100、200、400 mg//kg)對(duì)反枝莧(Amaranthus retroflexus L.)葉片光合色素、蛋白質(zhì)、丙二醛、脯氨酸含量和抗氧化酶(POD、CAT和SOD)活性的影響。結(jié)果表明:土壤鈾脅迫對(duì)反枝莧葉片光合色素和蛋白質(zhì)含量表現(xiàn)出低促高抑,25 mg/kg處理下葉綠素a、葉綠素b和蛋白質(zhì)含量顯著高于對(duì)照;中低濃度下,抗氧化酶系統(tǒng)被激活,活性顯著高于對(duì)照,有效清除活性氧自由基;100 mg/kg處理時(shí),SOD活性率先降低,引起CAT和POD活性出現(xiàn)無(wú)底物性下降;200、400 mg/kg脅迫下植物產(chǎn)生并積累了大量的自由基,超過(guò)了SOD、CAT、POD的清除能力,致使酶活性降低,膜脂過(guò)氧化程度加深,MDA含量激增;各處理下脯氨酸含量均顯著高于對(duì)照,使植物在高脅迫下抗氧化酶系統(tǒng)遭到破壞后仍能起到抗鈾脅迫作用。
[Abstract]:In order to investigate the stress resistance of uranium toxicity a.retroflexus inverse mechanism and uranium enrichment plant Amaranthus retroflexus, by pot experiment, the effects of soil with different concentrations of uranium (U) stress (0,25,50100200400 mg//kg) of a.retroflexus (Amaranthus retroflexus L.) Ye Pianguang pigment, protein, MDA, proline content and antioxidant enzymes (POD, CAT and SOD) were studied. The results showed that soil uranium stress of Amaranthus retroflexus L. leaf photosynthetic pigment and protein content showed a high low concentration under 25 mg/kg inhibition, chlorophyll a, chlorophyll b and protein content was significantly higher than that of control; low concentration, anti oxidase system is activated that was significantly higher than the control, effectively remove oxygen free radicals; 100 mg/kg treatment, SOD activity decreased, resulting in CAT and POD activities decreased substrate; under the stress of 200400 mg/kg plants produced and accumulated a large number of free radicals Over SOD, CAT and POD scavenging capacity resulted in decreased enzyme activity, increased membrane lipid peroxidation, and increased MDA content. The proline content in all treatments was significantly higher than that in control, so that plants could still play an anti uranium stress role when the antioxidant enzyme system was destroyed under high stress.
【作者單位】: 西南科技大學(xué)生命科學(xué)與工程學(xué)院;生物質(zhì)材料教育部工程研究中心;西南科技大學(xué)核廢物與環(huán)境安全國(guó)防重點(diǎn)學(xué)科實(shí)驗(yàn)室;
【基金】:國(guó)家核設(shè)施退役及放射性廢物治理科研重點(diǎn)項(xiàng)目(14ZG6101)
【分類號(hào)】:X173;X591
【正文快照】: 《環(huán)境科學(xué)與技術(shù)》編輯部:(網(wǎng)址)http://fjks.chinajournal.net.cn(電話)027-87643502(電子信箱)hjkxyjs@vip.126.com隨著國(guó)民經(jīng)濟(jì)的快速發(fā)展,核能在國(guó)防、工業(yè)、農(nóng)業(yè)、醫(yī)療和科研中廣泛應(yīng)用的同時(shí)也伴隨大量放射性廢棄物的產(chǎn)生。我國(guó)放射性廢棄物的年產(chǎn)量約為全國(guó)工業(yè)固體廢
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1 李方圓;內(nèi)生菌聯(lián)合反枝莧修復(fù)鉻污染土壤的研究[D];廣西大學(xué);2016年
2 王敏;太原市入侵植物反枝莧研究[D];山西大學(xué);2008年
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