發(fā)布時間：2018-01-06 10:08

  本文關(guān)鍵詞：基于GO-ZnS構(gòu)建的DNA傳感器用于環(huán)境優(yōu)先污染物的檢測　出處：《長沙理工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： GO-ZnS DNA傳感器 優(yōu)先污染物 鳥嘌呤(G) 電化學(xué)信號 響應(yīng)范圍

【摘要】：本文作者采用水熱法制備了氧化石墨烯-硫化鋅量子點(GO-ZnS)復(fù)合納米材料,利用掃描電鏡(SEM)、X射線衍射(XRD)對其表面形貌和成分進(jìn)行了分析,以獲得的GO-ZnS復(fù)合納米材料修飾玻碳電極(GCE),大大提高了玻碳電極的伏安響應(yīng)�；贕O-ZnS對電極界面電子傳導(dǎo)的增強(qiáng)效應(yīng)制備了一種新型的生物傳感器,實現(xiàn)了對環(huán)境優(yōu)先污染物的檢測。論文的主要研究內(nèi)容包括以下三個方面:比較了氧化石墨烯(GO)、納米鉑(Pt)、納米金-殼聚糖(Au-CTS)分別修飾玻碳電極(GCE)后的電化學(xué)交流阻抗行為,得到氧化石墨烯(GO)的修飾效果最佳。選用它作為交聯(lián)劑,當(dāng)GO和DNA的吸附濃度均為5mg/m L時,DNA的吸附電位和吸附時間分別為0.50V、200s時,通過計時電流法獲得的DNA/GO/GCE復(fù)合膜傳感器在pH=7.00的磷酸鹽緩沖溶液中(PBS)中對丙烯酰胺(AA)的響應(yīng)效果最佳,以差分脈沖伏安法(DPV)為測定方法,其電化學(xué)信號大小與丙烯酰胺(AA)的濃度對數(shù)呈現(xiàn)良好的線性關(guān)系,響應(yīng)范圍為5.0×10-8~1.0×10~(-3)mol/L,線性方程為△I=4.9286+0.4245lgc,相關(guān)系數(shù)為0.9989。采用水熱法制備的GO-ZnS復(fù)合納米材料,其SEM、XRD圖顯示ZnS量子點均勻地?fù)诫s到了GO中�？疾榱薌O、ZnS、GO-ZnS分別修飾玻碳電極(GCE)后獲得的DNA/GO/GCE、DNA/ZnS/GCE、DNA/GO-ZnS/GCE復(fù)合膜傳感器的差分脈沖伏安行為,得到GO-ZnS復(fù)合納米材料的修飾效果最佳。當(dāng)GO-ZnS和DNA的吸附濃度分別為6mg/mL、4mg/mL時,掃描速度為50mV/s時,DNA/GO-ZnS/GCE復(fù)合膜傳感器在pH=4.60的醋酸鹽緩沖溶液中對Ni化合物的響應(yīng)范圍為5.0×10~(-7)~1.0×10~(-3)mol/L,線性方程為△I=4.8176+0.4272lgc,相關(guān)系數(shù)為0.9949。采用電化學(xué)方法,發(fā)現(xiàn)DNA/GO-ZnS/GCE復(fù)合膜傳感器在pH=4.60的醋酸鹽緩沖溶液中對AA-Ni復(fù)合體系的響應(yīng)范圍為1.0×10~(-7)~1.0×10~(-3)mol/L,線性方程為△I=4.5333+0.3934lgc,相關(guān)系數(shù)為0.9931。該傳感器測定AA-Ni復(fù)合體系時所得的鳥嘌呤(G)的氧化峰電流變化差值(△I)略低于丙烯酰胺(AA)單一體系和Ni化合物單一體系的鳥嘌呤(G)的氧化峰電流變化差值(△I),且復(fù)合體系在pH=4.60的醋酸鹽緩沖液的響應(yīng)靈敏度明顯高于在pH=7.00的磷酸鹽緩沖溶液中(PBS)中的響應(yīng)靈敏度。
[Abstract]:The author of this paper by hydrothermal method oxidation graphene ZnS quantum dots were synthesized (GO-ZnS) nano composite materials, using scanning electron microscopy (SEM), X ray diffraction (XRD) on the surface morphology and composition were analyzed, in order to obtain the GO-ZnS nanocomposite modified glassy carbon electrode (GCE), greatly improved the voltammetric response of glassy carbon electrode. A new type of biosensor was prepared to enhance the effect of electrode interface electron transfer based on GO-ZnS, realized the detection of environmental priority pollutants. The main contents of this thesis include the following three aspects: the comparison of graphene oxide (GO), nano platinum, gold nanoparticles (Pt) chitosan (Au-CTS) were modified glassy carbon electrode (GCE) electrochemical impedance behavior of the obtained graphene oxide (GO) modification effect is the best. It used as a crosslinking agent, the adsorption concentration when GO and DNA were 5mg/m L, DNA of the adsorption potential and adsorption The time was 0.50V, 200s, DNA/GO/GCE composite film sensor by chronoamperometry obtained in pH=7.00 phosphate buffer solution (PBS) of acrylamide (AA) in response to the best effect by differential pulse voltammetry (DPV) measurement methods, the electrochemical signal size and concentration of acrylamide (AA) the log shows a good linear relationship, the response range is 5 * 10-8~1.0 * 10~ (-3) mol/L, the linear equation was I=4.9286+0.4245lgc, correlation coefficient is 0.9989. using GO-ZnS nanocomposites prepared by hydrothermal method, the SEM, XRD shows ZnS quantum dots uniformly doped in GO. ZnS, GO were studied. GO-ZnS modified glassy carbon electrode (GCE) for DNA/GO/GCE, DNA/ZnS/GCE, DNA/GO-ZnS/GCE composite film sensor by differential pulse voltammetry, get the modification effect of GO-ZnS nanocomposites. The best adsorption concentration when GO-ZnS and DNA respectively. 6mg/mL, 4mg/mL, scanning speed is 50mV/s, the DNA/GO-ZnS/GCE composite film sensor in acetate buffer solution pH=4.60 in the range of Ni compounds in response to 5 * 10~ (-7) ~1.0 * 10~ (-3) mol/L, the linear equation was I=4.8176+0.4272lgc, correlation coefficient is 0.9949. by electrochemical method, DNA/GO-ZnS/GCE composite film sensor acetate buffer solution pH=4.60 in the range of AA-Ni composite response is 1 * 10~ (-7) ~1.0 * 10~ (-3) mol/L, the linear equation was I=4.5333+0.3934lgc, correlation coefficient is determined from the composite system of AA-Ni 0.9931. sensor (G) guanine oxidation peak current difference (delta I) slightly lower than acrylamide (AA) single system and Ni compound system with single guanine (G) oxidation peak current difference (delta I), and the response sensitivity in acetate buffer solution of pH=4.60 composites was significantly higher than that of Response sensitivity in the phosphate buffer solution (PBS) of pH=7.00.

【學(xué)位授予單位】：長沙理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X830;O657.1

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