本文關(guān)鍵詞：DDT高效降解菌在室內(nèi)模擬修復(fù)中的應(yīng)用及其降解機(jī)理　出處：《哈爾濱師范大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： DDT 室內(nèi)模擬降解 固定化 降解途徑

【摘要】：本研究以8株具有DDT降解能力的細(xì)菌為對(duì)象,通過(guò)馴化提高其降解率,從中選擇降解率最高的菌株研究其降解特性,以正交實(shí)驗(yàn)獲得各菌株最適降解條件,研究降解途徑,分別進(jìn)行固定化處理,通過(guò)正交實(shí)驗(yàn)獲得最佳降解條件并構(gòu)建混合菌,模擬自然條件構(gòu)建室內(nèi)模擬降解系統(tǒng),以固定化混合菌株作為研究對(duì)象,探究其在室內(nèi)模擬降解條件下對(duì)DDT污染土壤的降解效果。研究結(jié)果表明,8菌株的降解率分別由馴化前的31.23%、24.50%、20.68%、35.70%、51.60%、18.40%、40.45%、30.65%提高至35.23%、26.40%、22.90%、40.10%、55.60%、19.50%、46.23%、36.80%。降解率最高的3個(gè)菌株為12-2#、12-3#與23#。菌株12-2#的最佳降解條件是:溫度32℃、初始pH=7.0、菌體添加量6 mL,最大降解率40.10%;菌株12-3#的最佳降解條件是:溫度34℃、初始pH=7.0、菌體添加量8 mL,最大降解率55.60%;菌株23#的最佳降解條件是:溫度32℃、初始pH=7.0、菌體添加量6 mL,最大降解率46.23%。3菌株對(duì)DDT的降解途徑為:好氧條件下,通過(guò)2,3-雙加氧酶、順式-2,3-二氫二醇DDT脫氫酶及2,3-二羥基DDT1,2-雙加氧酶等的作用,將DDT直接開(kāi)環(huán),最終生成4-氯苯甲酸。固定化處理使3菌株的降解率分別由40.10%、55.60%、46.23%提高至54.46%、70.07%、61.98%。菌株12-2#、12-3#與23#固定化小球的最佳制備條件分別為:海藻酸鈉濃度2%,氯化鈣濃度2%,活性炭添加量6 g/L,菌體添加量4 mL(OD600=1.0),交聯(lián)時(shí)間8 h;海藻酸鈉濃度3%,氯化鈣濃度2%,活性炭添加量6 g/L,菌體添加量4 mL(OD600=1.0),交聯(lián)時(shí)間8 h;海藻酸鈉濃度2%,氯化鈣濃度2%,活性炭添加量6 g/L,菌體添加量3 mL(OD600=1.0),交聯(lián)時(shí)間8 h時(shí)。菌株12-2#、12-3#與23#固定化小球重復(fù)利用3次后,降解率未見(jiàn)顯著降低(P0.05);低溫存儲(chǔ)45 d后,降解率僅分別降低4.23%、3.33%與4.52%�；旌暇顑�(yōu)構(gòu)建比例為12-2#:12-3#:23#=1:3:2;最適降解條件為:溫度32℃、初始pH=7.0、菌體添加量8 mL,最大降解率為58.43%�；旌暇潭ɑ∏虻淖罴阎苽錀l件為:海藻酸鈉濃度3%,氯化鈣濃度2%,活性炭添加量10 g/L,菌體添加量3mL(OD600=1.0),交聯(lián)時(shí)間8 h;最佳降解條件為:溫度32℃、初始pH=7.0、添加量10.0 g,最大降解率70.23%;室內(nèi)模擬條件下最大降解率49.99%。
[Abstract]:In this study, 8 strains with DDT degrading bacteria, improve the degradation rate through domestication, choose to study the degradation characteristics of the highest rate of degradation from the strains, with the orthogonal experiment to obtain the optimum degradation conditions of the strains, study the degradation pathway, were immobilized by orthogonal experiment, optimal degradation conditions and construct mixed bacteria, simulating the natural conditions of building indoor simulation degradation system with Immobilized Mixed Bacteria as the research object, to explore the effect of simulation on DDT contaminated soil degradation under laboratory condition. The results showed that the degradation rate of 8 strains increased from 31.23%, 24.50%, 20.68%, 35.70%, 51.60%, 18.40%, 40.45%, 30.65% to 35.23%, 26.40%, 26.40%, 26.40%, 26.40%, 26.40%, 40.45%, 30.65%, respectively. The 3 strains with the highest degradation rate were 12-2#, 12-3# and 23#. The optimal degradation conditions of strain 12-2# is: the temperature of 32 DEG C, pH=7.0, initial cell concentration of 6 mL, the maximum degradation rate of 40.10%; the optimal degradation conditions of 12-3# were: the temperature of 34 DEG C, pH=7.0, initial cell concentration of 8 mL, the maximum degradation rate of 55.60%; strain 23# optimal degradation condition is: the temperature of 32 DEG C pH=7.0, the initial cell concentration of 6 mL, the maximum degradation rate of 46.23%. 3, the way of DDT degradation is: under aerobic condition, the 2,3- is directly opened by ring opening of DDT through the action of 2,3- dioxygenase, CIS -2,3- two hydrogen glycol DDT dehydrogenase and 2,3- two hydroxyl DDT1,2- double oxygenase. Finally, 4- chlorobenzoic acid is generated. The degradation rate of 3 strains was increased from 40.10%, 55.60% and 46.23% to 54.46%, 70.07% and 61.98% by immobilized treatment. Preparation conditions of strains 12-2#, 12-3# and 23# were immobilized respectively: 2% sodium alginate concentration, calcium chloride concentration was 2%, the dosage of activated carbon dosage was 6 g/L, 4 mL (OD600=1.0), crosslinking time of 8 h; 3% sodium alginate concentration, calcium chloride concentration was 2%, the dosage of activated carbon 6 g/L, add the amount of mL was 4 (OD600=1.0), crosslinking time of 8 h; 2% sodium alginate concentration, calcium chloride concentration was 2%, the dosage of activated carbon dosage was 6 g/L, 3 mL (OD600=1.0), 8 h crosslinking time. After 3 times of repeated use of strain 12-2#, 12-3# and 23#, the degradation rate was not significantly reduced (P0.05). After storage for 45 d at low temperature, the degradation rate decreased by 4.23%, 3.33% and 4.52%, respectively. The best proportion of mixed bacteria was 12-2#: 12-3#: 23#=1:3:2; the best degradation conditions were: 32 degree temperature, initial pH=7.0, adding 8 mL, and the maximum degradation rate was 58.43%. The optimum preparation conditions for mixed immobilized microorganisms: 3% sodium alginate, calcium chloride concentration was 2%, the dosage of activated carbon was 10 g/L, addition of 3mL (OD600=1.0), crosslinking time of 8 h; the optimal degradation conditions: temperature 32, initial pH=7.0, dosage of 10 g, the maximum degradation rate of 70.23%; the maximum degradation rate of 49.99% under the condition of indoor simulation.
【學(xué)位授予單位】：哈爾濱師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X592;X172

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