稀土摻雜的納米晶的合成與生物成像應用的研究
發(fā)布時間：2018-08-19 06:06
【摘要】：稀土摻雜的納米晶由于其獨特的發(fā)光性質,在生物成像和生物探針領域有著非常廣闊的應用前景。但稀土納米晶本身對于待檢測離子不具有響應性,需要和有機小分子相結合。構建稀土納米晶和有機小分子的復合體系,進行活體內的近紅外發(fā)光探針的研究,是目前研究的熱點。本文通過在稀土納米晶表面引入介孔二氧化硅殼層作為載體,將有機小分子染料載入介孔二氧化硅殼層,設計合成了近紅外光激發(fā)的活體次氯酸根檢測的納米探針,應用于小動物活體內的炎癥模型的檢測。為了進一步提高穿透深度,我們合成了Nd3+摻雜的稀土下轉換納米晶,用于活體的肝脾和淋巴結的光學和磁共振雙模式成像。1、上轉換納米體系用于體外和體內次氯酸的靈敏檢測我們設計了Er3+、Tm3+共摻雜的稀土上轉換發(fā)光納米粒子、介孔二氧化硅殼層和h Cy5.5有機小分子染料構成的三層復合體系,用于HOCl/Cl O-的檢測。并將這一體系用于細胞和活體水平上的HOCl/Cl O-檢測。然后建立了昆明鼠(帶毛)關節(jié)炎的炎癥模型,將材料用于小鼠關節(jié)炎的檢測,實現(xiàn)了細胞和活體內的上轉換成像和比度成像。2、PEG修飾的Na YF4:Nd@Na Gd F4納米晶用于下轉換和磁共振雙模成像的研究Nd3+摻雜的稀土下轉換納米晶相比于980 nm激發(fā)的上轉換納米粒子有著更高的穿透深度和比較低的熱效應。我們合成了不同Nd3+摻雜比的Na YF4:Nd,并在外層包覆一層Na Gd F4殼層,然后篩選出了發(fā)光性質最優(yōu)的核殼結構摻雜比。并將核殼結構的Na YF4:Nd@Na Gd F4納米晶用親水性的PEG修飾過以后,用于小鼠的肝脾成像和熒光指導淋巴結切除手術。由于有Na Gd F4殼層的包覆,材料能夠用于磁共振成像,實現(xiàn)了熒光和磁共振的雙模成像。
[Abstract]:Rare earth doped nanocrystals have a broad application prospect in the field of biological imaging and biological probe due to their unique luminescent properties. However, rare earth nanocrystals are not responsive to the ions to be detected and need to be combined with small organic molecules. It is a hot topic to construct the composite system of rare earth nanocrystalline and organic small molecule and study the near infrared luminescence probe in vivo. In this paper, the mesoporous silica shell was introduced on the surface of rare earth nanocrystals as the carrier, and the organic small molecular dyes were loaded into the mesoporous silica shell layer. A near-infrared photoexcited nano-probe for the detection of hypochlorite was designed and synthesized. It is applied to the detection of inflammatory model in vivo in small animals. In order to further improve the penetration depth, Nd3 doped rare earth downconversion nanocrystals were synthesized. Optical and Magnetic Resonance Dual-Mode Imaging for liver-spleen and lymph nodes in Vivo. The up-conversion nanocrystalline system was used for sensitive detection of hypochloric acid in vitro and in vivo. We designed Er3 Tm3 co-doped rare-earth up-conversion luminescent nanoparticles. A three-layer composite system composed of mesoporous silica shell and h Cy5.5 organic small molecular dyes was used for the detection of HOCl/Cl O-. The system was used for the detection of HOCl/Cl O-at the cellular and in vivo levels. Then the inflammatory model of Kunming mouse (hairy) arthritis was established, and the material was used for the detection of mouse arthritis. Up-conversion of cells and in vivo and specific imaging of PEG-modified Na YF4:Nd@Na GdF4 nanocrystals for downconversion and magnetic resonance dual-mode imaging; Nd3 doped rare-earth downconversion nanocrystals compared to 980nm excited upconversion Nanoparticles have higher penetration depth and lower thermal effects. We synthesized NaYF4: Ndwith different doping ratios of Nd3 and coated a layer of NaGdF4 shell with the outer layer. Then we screened out the optimum doping ratio of core-shell structure for luminescent properties. Na YF4:Nd@Na Gd F 4 nanocrystals with core-shell structure were modified with hydrophilic PEG for liver and spleen imaging and fluorescence guided lymph node excision in mice. Because of the coating of Na Gd F 4 shell, the material can be used in magnetic resonance imaging, and the fluorescence and magnetic resonance double mode imaging can be realized.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TQ133.3;TB383.1
本文編號：2190866
[Abstract]:Rare earth doped nanocrystals have a broad application prospect in the field of biological imaging and biological probe due to their unique luminescent properties. However, rare earth nanocrystals are not responsive to the ions to be detected and need to be combined with small organic molecules. It is a hot topic to construct the composite system of rare earth nanocrystalline and organic small molecule and study the near infrared luminescence probe in vivo. In this paper, the mesoporous silica shell was introduced on the surface of rare earth nanocrystals as the carrier, and the organic small molecular dyes were loaded into the mesoporous silica shell layer. A near-infrared photoexcited nano-probe for the detection of hypochlorite was designed and synthesized. It is applied to the detection of inflammatory model in vivo in small animals. In order to further improve the penetration depth, Nd3 doped rare earth downconversion nanocrystals were synthesized. Optical and Magnetic Resonance Dual-Mode Imaging for liver-spleen and lymph nodes in Vivo. The up-conversion nanocrystalline system was used for sensitive detection of hypochloric acid in vitro and in vivo. We designed Er3 Tm3 co-doped rare-earth up-conversion luminescent nanoparticles. A three-layer composite system composed of mesoporous silica shell and h Cy5.5 organic small molecular dyes was used for the detection of HOCl/Cl O-. The system was used for the detection of HOCl/Cl O-at the cellular and in vivo levels. Then the inflammatory model of Kunming mouse (hairy) arthritis was established, and the material was used for the detection of mouse arthritis. Up-conversion of cells and in vivo and specific imaging of PEG-modified Na YF4:Nd@Na GdF4 nanocrystals for downconversion and magnetic resonance dual-mode imaging; Nd3 doped rare-earth downconversion nanocrystals compared to 980nm excited upconversion Nanoparticles have higher penetration depth and lower thermal effects. We synthesized NaYF4: Ndwith different doping ratios of Nd3 and coated a layer of NaGdF4 shell with the outer layer. Then we screened out the optimum doping ratio of core-shell structure for luminescent properties. Na YF4:Nd@Na Gd F 4 nanocrystals with core-shell structure were modified with hydrophilic PEG for liver and spleen imaging and fluorescence guided lymph node excision in mice. Because of the coating of Na Gd F 4 shell, the material can be used in magnetic resonance imaging, and the fluorescence and magnetic resonance double mode imaging can be realized.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TQ133.3;TB383.1
【參考文獻】
相關期刊論文 前2條
1 李艷紅;張永明;馬晶;陸海燕;馬銘遠;;GdF_3∶Eu~(3+)/NaGdF_4∶Eu~(3+)納米晶的水熱合成及發(fā)光性質[J];無機化學學報;2011年06期
2 ;Preparation and luminescence properties of monodisperse silica/aminosilane-coated Y_2O_3:Yb,Ho upconversion nanoparticles[J];Chinese Science Bulletin;2011年02期
，本文編號：2190866
本文鏈接：http://sikaile.net/kejilunwen/huagong/2190866.html
