發(fā)布時(shí)間：2018-03-05 04:31

  本文選題：預(yù)處理　切入點(diǎn)：固定化　出處：《化學(xué)工程》2017年07期 　論文類型：期刊論文

【摘要】：考察經(jīng)多種天然添加劑、活性劑成分及有機(jī)相反應(yīng)前預(yù)處理實(shí)驗(yàn)室自制固定化酶膜對(duì)十二酸十二醇、維生素A/C棕櫚酸酯、棕櫚酸異辛酯等酯化合成的影響。結(jié)果發(fā)現(xiàn):經(jīng)過明膠、多羥基化合物PVP及含活性劑成分復(fù)配乳液PDMS等處理后,酶膜對(duì)十二酸十二醇酯化酶活有1.2—1.5倍不同程度提高;相比凍干酶粉完全失活含質(zhì)量分?jǐn)?shù)98%PEG6000的凍干酶粉催化酯化率在70%以上,結(jié)合鹽及聚乙二醇高分子化合物對(duì)酶保護(hù)作用,該方法適于凍干Candida sp.99-125脂肪酶粉的預(yù)處理;而在所選常用有機(jī)溶劑中酶活性穩(wěn)定,可用于反應(yīng)后酶膜的再生。在棕櫚酸異辛酯的酯化合成中,復(fù)合乳劑PDMS對(duì)酶穩(wěn)定性沒有影響,而天然羥基化合物PVP及乳糖明顯有利于酶活性的保持、酶重復(fù)使用次數(shù)的增加。
[Abstract]:The immobilized enzyme membrane was pretreated with various natural additives, active agent components and organic phase reaction. The immobilized enzyme membrane was prepared for the treatment of 12 alcohol acid, vitamin A / C palmitate ester by immobilized enzyme membrane. Effects of isooctyl palmitate on esterification and synthesis. The results showed that after treatment with gelatin, polyhydroxyl compound PVP and emulsion PDMS containing active agent, the enzymatic activity of 12 acid 12 alcohols was increased by 1.2-1.5 times. Compared with the lyophilized enzyme powder containing 98% PEG6000, the catalytic esterification rate of the freeze-dried enzyme powder was over 70%. The method was suitable for pretreatment of freeze-dried Candida sp.99-125 lipase powder combined with the protective effect of salt and polyethylene glycol on the enzyme. The enzyme activity was stable in common organic solvents and could be used to regenerate the enzyme membrane after reaction. In esterification synthesis of isooctyl palmitate, the composite emulsion PDMS had no effect on the stability of the enzyme. The natural hydroxyl compounds PVP and lactose were beneficial to keep the enzyme activity, and the times of repeated use of enzyme were increased.
【作者單位】： 陜西省可降解生物醫(yī)用材料重點(diǎn)實(shí)驗(yàn)室;西北大學(xué)化工學(xué)院陜西省生物材料與發(fā)酵工程技術(shù)研究中心;北京化工大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：陜西省教育廳重點(diǎn)實(shí)驗(yàn)室科研計(jì)劃項(xiàng)目(16JS104) 中國(guó)博士后科學(xué)基金面上資助(2015M582698) 西北大學(xué)科學(xué)研究基金(15NW14)
【分類號(hào)】：Q814.2;TQ032.4

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