本文選題：乳山灣　切入點(diǎn)：潛在硝化速率　出處：《中國(guó)環(huán)境科學(xué)》2017年03期 　論文類型：期刊論文

【摘要】：在測(cè)定乳山灣近海沉積物潛在硝化速率(PNR)的過(guò)程中發(fā)現(xiàn),培養(yǎng)前后溶解無(wú)機(jī)氮(DIN)含量顯著下降,表明體系中存在DIN損失,且損失的溶解無(wú)機(jī)氮含量與硝化總量的比值為2.72%~40.02%.進(jìn)一步通過(guò)實(shí)時(shí)熒光定量PCR技術(shù)測(cè)定培養(yǎng)過(guò)程中亞硝酸鹽還原酶基因(nitrite reductase gene,nirK)的表達(dá)情況,發(fā)現(xiàn)氨氧化古菌(AOA)和好氧氨氧化細(xì)菌(AOB)均有nirK基因的表達(dá),表明硝化微生物的反硝化過(guò)程(ND)是導(dǎo)致無(wú)機(jī)氮損失的原因之一.若僅根據(jù)測(cè)得的硝酸鹽和亞硝酸鹽濃度估算乳山灣近海沉積物的潛在硝化速率會(huì)低估PNR(C0和C2 2個(gè)站位,考慮ND過(guò)程得到的總PNR分別是未考慮ND過(guò)程的15.9倍和22.1倍,而對(duì)于AOA的PNR則是22.3倍和46.1倍),因此在計(jì)算時(shí),必須將體系中損失的無(wú)機(jī)氮計(jì)算在內(nèi).
[Abstract]:During the determination of the potential nitrification rate of sediments in Rushan Bay, it was found that the content of dissolved inorganic nitrogen (DINs) decreased significantly before and after culture, indicating that there was a loss of DIN in the system. The ratio of dissolved inorganic nitrogen to nitrification was 2.72% and 40.02% respectively. The expression of nitrite reductase gene gene nitrite reductase gene nitrite gene nirk was determined by real-time fluorescence quantitative PCR. It was found that nirK gene was expressed in both AOAA (AOAA) and AOB (aerobic ammonia-oxidizing bacteria). The results showed that the denitrification process of nitrifying microorganism (NDN) was one of the reasons leading to the loss of inorganic nitrogen. The estimation of the potential nitrification rate in the offshore sediments of Rushan Bay based on the measured nitrate and nitrite concentrations would underestimate the PNR(C0 and C 2 stations. The total PNR obtained by considering ND process is 15.9 times and 22.1 times as much as that without ND process, while the PNR of AOA is 22.3 times and 46.1 times higher than that without ND process. Therefore, the inorganic nitrogen loss in the system must be taken into account in the calculation.
【作者單位】： 中國(guó)海洋大學(xué)海洋生命學(xué)院;中國(guó)海洋大學(xué)海洋環(huán)境與生態(tài)教育部重點(diǎn)實(shí)驗(yàn)室;青島海洋科學(xué)與技術(shù)國(guó)家實(shí)驗(yàn)室海洋生態(tài)與環(huán)境科學(xué)功能實(shí)驗(yàn)室;中國(guó)海洋大學(xué)環(huán)境科學(xué)與工程學(xué)院;中國(guó)海洋大學(xué)海洋化學(xué)理論與教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(41620104001,41521064) 中國(guó)科學(xué)院海洋生態(tài)與環(huán)境科學(xué)重點(diǎn)實(shí)驗(yàn)室 青島海洋科學(xué)與技術(shù)國(guó)家實(shí)驗(yàn)室海洋生態(tài)與環(huán)境科學(xué)開放課題(KLMEES201601)
【分類號(hào)】：X55;X834

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