近年來,慢性病的患病率急劇上升,威脅著人類健康,影響著人們的生活質(zhì)量�，F(xiàn)有的臨床檢測技術(shù)成本高、耗時(shí)長,無法實(shí)現(xiàn)對慢性病的低成本快速檢測。石墨烯場效應(yīng)晶體管（Graphene Field Effect Transistor,GFET）納米生物傳感器具有靈敏度高、響應(yīng)快等優(yōu)點(diǎn),在疾病預(yù)防和診斷中具有廣闊的應(yīng)用前景。本文介紹了一種基于適配型GFET傳感器的平板液柵型GFET納米生物傳感器的設(shè)計(jì)與制作。通過理論分析和實(shí)驗(yàn)研究了核酸適體蛋白親和結(jié)合對石墨烯中載流子濃度和Dirac點(diǎn)位置的影響,從而揭示了GFET納米生物傳感器對蛋白質(zhì)生物標(biāo)志物檢測的傳感機(jī)理。利用Hill方程和GFET輸運(yùn)特性建立了GFET納米生物傳感器響應(yīng)信號(hào)與蛋白質(zhì)濃度之間的數(shù)學(xué)模型,為解釋核酸適體蛋白親和結(jié)合效應(yīng)對石墨烯漏源電流的影響提供了理論和實(shí)驗(yàn)支持。以血紅蛋白分子為主要研究對象,利用GFET納米生物傳感器在人工磷酸鹽溶液（PBS）和生物（血清）樣本中對血紅蛋白生物標(biāo)記物進(jìn)行了檢測實(shí)驗(yàn),以證明該核酸適配體與蛋白質(zhì)分子具有良好的結(jié)合親和力。利用拉曼光譜、能譜儀（Energy Dispersive Spectrometer... 

【文章來源】：哈爾濱工業(yè)大學(xué)黑龍江省 211工程院校 985工程院校

【文章頁數(shù)】：64 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
Abstract
CHAPTER1 INTRODUCTION
    1.1 Introduction and Motivations
    1.2 Research Background and Significance
        1.2.1 Graphene,its properties and production
        1.2.2 Graphene field effect transistors,their structure and benefits
        1.2.3 Fabrication of GFETs
        1.2.4 Aptamer
        1.2.5 Significance
    1.3 Hemoglobin Characterization
        1.3.1 Hemoglobin structure and function
        1.3.2 Conditions with a low Hemoglobin
        1.3.3 Conditions with an elevated Hemoglobin
        1.3.4 Abnormal Hemoglobin
    1.4 Main research contents
CHAPTER2 THEORETICAL ANALYSIS OF THE SENSING PRINCIPLE OF GFET NANOBIOSENSOR
    2.1 Introduction
    2.2 Electric transport characteristics of GFET sensor
    2.3 Principle of the nucleic acid aptamer GFET sensor detection
    2.4 Mathematical model of the GFET sensor response signal and Hb concentrations
    2.5 Summary
CHAPTER3 DESIGN AND FABRICATION OF THE GFET NANOBIOSENSOR
    3.1 Introduction
    3.2 The optimal transfer method of CVD graphene
    3.3 MEMS processing technology based design and fabrication of GFET sensor with a planar gate
    3.4 Characterization of the graphene functionalization
        3.4.1 Raman characterization of the PASE functionalization
        3.4.2 EDS characterization of the aptamer functionalization
        3.4.3 Electrical characterization of PASE and aptamer functionalization
    3.5 Summary
CHAPTER4 RESPONSE CHARACTERISTICS OF THE GFET NANOBIOSENSOR TO PROTEINS
    4.1 Introduction
    4.2 Protein detection in PBS using GFET nanobiosensor
        4.2.1 Control experiments of protein detection without and with aptamer functionalization
        4.2.2 Response characteristics of the GFET nanobiosensor to hemoglobin molecule
    4.3 Detection of Hemoglobin in Serum using GFET nanobiosensor
    4.4 Summary
CONCLUSION
REFERENCES
致謝



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