無(wú)透鏡數(shù)字全息成像觀測(cè)實(shí)驗(yàn)系統(tǒng)的研制
發(fā)布時(shí)間：2019-04-19 01:05
【摘要】：數(shù)字全息技術(shù)是當(dāng)今科研領(lǐng)域研究的一個(gè)熱門方向。無(wú)透鏡數(shù)字全息是對(duì)傳統(tǒng)的數(shù)字全息成像的一個(gè)改進(jìn),它在簡(jiǎn)化光路的同時(shí)保證了全息圖的效果。特別是在細(xì)胞觀測(cè)、微流控等領(lǐng)域具有很高的實(shí)用價(jià)值。當(dāng)前對(duì)無(wú)透鏡數(shù)字全息成像的研究較少,少數(shù)的無(wú)透鏡數(shù)字全息成像實(shí)驗(yàn)研究雖然能得到較好的成像效果,但對(duì)與成像效果至關(guān)重要的條件(如光源顏色、波長(zhǎng)、光源到光電傳感器距離等)缺乏規(guī)律性的總結(jié),給應(yīng)用帶來了不便。此外,市面上也還沒有將數(shù)字全息重建與圖像處理相結(jié)合的軟件,可以實(shí)現(xiàn)如全息圖的重建、再現(xiàn)像的特征提取、測(cè)量、分割等對(duì)于細(xì)胞觀測(cè)比較重要的功能。現(xiàn)有方法對(duì)圖像的處理往往通過Matlab來進(jìn)行,效率低、操作步驟不夠直觀,且難以實(shí)現(xiàn)商業(yè)化。基于此,本文搭建了無(wú)透鏡數(shù)字全息成像的硬件裝置,利用Visual C++開發(fā)了相應(yīng)的圖像處理軟件,最終形成一個(gè)完整的無(wú)透鏡數(shù)字全息觀測(cè)實(shí)驗(yàn)系統(tǒng),并對(duì)無(wú)透鏡數(shù)字全息成像的成像條件進(jìn)行了探索。本文完成的工作內(nèi)容包括以下三個(gè)方面:①對(duì)數(shù)字全息重建算法進(jìn)行了深入研究,并選擇卷積重建算法作為全息圖的重建算法,在驗(yàn)證了該算法的準(zhǔn)確性之后,采用Visual C++開發(fā)了一套無(wú)透鏡數(shù)字全息采集與處理系統(tǒng),實(shí)現(xiàn)了數(shù)字全息圖的采集、重建和圖像的濾波、變換分割、形態(tài)學(xué)處理等功能。同時(shí)本系統(tǒng)可移值、可擴(kuò)展,并且操作簡(jiǎn)便、界面美觀。②設(shè)計(jì)并搭建了實(shí)驗(yàn)裝置平臺(tái),通過步機(jī)電機(jī)和傳感器與無(wú)透鏡數(shù)字全息采集與處理軟件結(jié)合,實(shí)現(xiàn)軟件與硬件相結(jié)合來控制實(shí)驗(yàn)條件的功能,主要完成了光源到CMOS距離和光源開關(guān)的軟件控制、成像條件的軟件記錄等。③基于整個(gè)實(shí)驗(yàn)裝置和軟件系統(tǒng),設(shè)計(jì)了最佳成像條件探索、乳腺癌細(xì)胞和人血紅細(xì)胞觀測(cè)這三個(gè)不同的實(shí)驗(yàn)來進(jìn)行系統(tǒng)的可行性驗(yàn)證。實(shí)驗(yàn)結(jié)果表明:波長(zhǎng)為465?470 nm、亮度為8000?10000 nit、角度為20度的粉紅光,光源到CMOS距離為5.5?7.5 cm范圍時(shí),采集到的全息圖效果最好。通過無(wú)透鏡數(shù)字全息采集與處理軟件,成功采集并重建了乳腺癌細(xì)胞和人血紅細(xì)胞,細(xì)胞的位置與輪廓清楚。在此軟件上對(duì)細(xì)胞進(jìn)行測(cè)量和計(jì)數(shù)結(jié)果顯示,其大小和細(xì)胞的實(shí)際大小相近,而計(jì)數(shù)結(jié)果也和顯微鏡下相差不大,驗(yàn)證了整個(gè)系統(tǒng)的可行性及合理性。
[Abstract]:Digital holography is a hot research direction in the field of scientific research. Lens-free digital holography is an improvement of traditional digital holography, which not only simplifies the optical path but also guarantees the effect of hologram. Especially in cell observation, microfluidic and other fields have high practical value. At present, there are few researches on lensless digital holography. Although a few experimental researches on lensless digital holography can get better imaging results, they are very important to the imaging effect (such as light source color, wavelength, etc.) The distance from light source to photoelectric sensor is lack of regular summary, which brings inconvenience to application. In addition, there is no software to combine digital holography reconstruction with image processing on the market, which can realize the important functions of cell observation such as hologram reconstruction, feature extraction, measurement, segmentation and so on. The existing methods for image processing are often carried out through Matlab, which is inefficient, the operation steps are not intuitive enough, and it is difficult to commercialize the image. Based on this, the hardware device of lensless digital holography is built, and the corresponding image processing software is developed by using Visual C, and finally a complete experimental system of lensless digital holography is formed. The imaging conditions of lensless digital holography are explored. The main contents of this paper are as follows: 1. The digital holography reconstruction algorithm is studied deeply, and convolution reconstruction algorithm is chosen as the reconstruction algorithm of hologram. After validating the accuracy of this algorithm, A set of lensless digital holography acquisition and processing system is developed with Visual C. the functions of digital hologram collection, reconstruction, image filtering, transform segmentation, morphological processing and so on are realized. At the same time, the system can be moved, expandable, easy to operate, and the interface is beautiful. 2 the experimental device platform is designed and built, which is combined with lensless digital holography acquisition and processing software by stepping motor, sensor and lensless digital holography. The function of combining software and hardware to control the experimental conditions is realized, including the software control of the distance from the light source to the CMOS and the switch of the light source, the software recording of the imaging conditions, etc. 3Based on the whole experimental device and software system, The optimal imaging conditions, breast cancer cells and human red blood cells were designed to verify the feasibility of the system. The experimental results show that when the wavelength is 465? 470 nm, and the luminance is 8 000? 10 000 nit, the angle is 20?, and the distance from the light source to the CMOS is 5.5? 7.5 cm, the result of the hologram is the best. Through lens-free digital holography acquisition and processing software, breast cancer cells and human red blood cells were successfully collected and reconstructed. The location and outline of the cells were clear. The results of measuring and counting the cells on this software show that the size of the cells is similar to the actual size of the cells, and the counting results are not much different from those under the microscope, which verifies the feasibility and rationality of the whole system.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TN26
[Abstract]:Digital holography is a hot research direction in the field of scientific research. Lens-free digital holography is an improvement of traditional digital holography, which not only simplifies the optical path but also guarantees the effect of hologram. Especially in cell observation, microfluidic and other fields have high practical value. At present, there are few researches on lensless digital holography. Although a few experimental researches on lensless digital holography can get better imaging results, they are very important to the imaging effect (such as light source color, wavelength, etc.) The distance from light source to photoelectric sensor is lack of regular summary, which brings inconvenience to application. In addition, there is no software to combine digital holography reconstruction with image processing on the market, which can realize the important functions of cell observation such as hologram reconstruction, feature extraction, measurement, segmentation and so on. The existing methods for image processing are often carried out through Matlab, which is inefficient, the operation steps are not intuitive enough, and it is difficult to commercialize the image. Based on this, the hardware device of lensless digital holography is built, and the corresponding image processing software is developed by using Visual C, and finally a complete experimental system of lensless digital holography is formed. The imaging conditions of lensless digital holography are explored. The main contents of this paper are as follows: 1. The digital holography reconstruction algorithm is studied deeply, and convolution reconstruction algorithm is chosen as the reconstruction algorithm of hologram. After validating the accuracy of this algorithm, A set of lensless digital holography acquisition and processing system is developed with Visual C. the functions of digital hologram collection, reconstruction, image filtering, transform segmentation, morphological processing and so on are realized. At the same time, the system can be moved, expandable, easy to operate, and the interface is beautiful. 2 the experimental device platform is designed and built, which is combined with lensless digital holography acquisition and processing software by stepping motor, sensor and lensless digital holography. The function of combining software and hardware to control the experimental conditions is realized, including the software control of the distance from the light source to the CMOS and the switch of the light source, the software recording of the imaging conditions, etc. 3Based on the whole experimental device and software system, The optimal imaging conditions, breast cancer cells and human red blood cells were designed to verify the feasibility of the system. The experimental results show that when the wavelength is 465? 470 nm, and the luminance is 8 000? 10 000 nit, the angle is 20?, and the distance from the light source to the CMOS is 5.5? 7.5 cm, the result of the hologram is the best. Through lens-free digital holography acquisition and processing software, breast cancer cells and human red blood cells were successfully collected and reconstructed. The location and outline of the cells were clear. The results of measuring and counting the cells on this software show that the size of the cells is similar to the actual size of the cells, and the counting results are not much different from those under the microscope, which verifies the feasibility and rationality of the whole system.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TN26
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