發(fā)布時(shí)間：2018-05-20 18:22

  本文選題：介電電泳 + 印制電路板�。� 參考：《重慶大學(xué)》2015年碩士論文

【摘要】：介電電泳技術(shù)是微流控系統(tǒng)中實(shí)現(xiàn)粒子富集與分離的關(guān)鍵技術(shù)之一;與機(jī)械或光學(xué)等其他方法相比,具有操作方便、響應(yīng)速度快、非接觸、易于實(shí)現(xiàn)微型化和自動(dòng)化等優(yōu)點(diǎn),在一些大分子微粒及生物細(xì)胞的準(zhǔn)確捕獲、富集、分離及輸運(yùn)研究中具有極大的潛力�，F(xiàn)有介電泳芯片大多通過微機(jī)電系統(tǒng)(Micro-Electro-Mechanical System,MEMS)技術(shù)加工而成,但該工藝設(shè)備昂貴、工序復(fù)雜,目前不能實(shí)現(xiàn)批量化生產(chǎn),限制了芯片的推廣應(yīng)用。而印制電路板(Printed Circuit Board,PCB)工藝具有低成本、可批量生產(chǎn)、便于封裝等優(yōu)點(diǎn),研究采用PCB工藝加工介電泳芯片并實(shí)現(xiàn)粒子操控具有重要意義與實(shí)用價(jià)值。本文針對(duì)PCB工藝分辨率存在局限性的問題,圍繞基于PCB工藝的交流介電泳芯片優(yōu)化設(shè)計(jì)與利用該芯片實(shí)現(xiàn)粒子富集與分離的原理和實(shí)驗(yàn)展開了研究。具體包括以下幾個(gè)方面的工作:①分析了介電電泳技術(shù)操控粒子富集與分離的原理。對(duì)非均勻電場(chǎng)作用下懸浮于電介質(zhì)溶液中的中性微粒進(jìn)行了受力分析,得到介電泳力操控粒子運(yùn)動(dòng)的主要影響因素。②建立叉指式介電泳芯片的二維仿真模型,在PCB工藝分辨率允許的條件下,模擬了芯片中電場(chǎng)的分布情況。進(jìn)一步分析了芯片中粒子受到介電泳力的大小和方向,獲得了通道高度、電極特征參數(shù)(電極寬度/間距)及電壓與介電泳力大小的關(guān)系,優(yōu)化了介電泳芯片的電極參數(shù)。③基于Laplace方程,采用傅里葉級(jí)數(shù)分析方法,推導(dǎo)了影響介電電泳分離的關(guān)鍵參數(shù)—粒子懸浮高度的表達(dá)式;通過與仿真結(jié)果對(duì)比,對(duì)計(jì)算結(jié)果進(jìn)行了驗(yàn)證。進(jìn)一步分析得到電極厚度對(duì)粒子懸浮高度的影響隨著電極厚度的增大而增大。④通過PCB工藝制作了電極寬度/間距為100μm的叉指式介電泳芯片,以聚苯乙烯微球和酵母菌細(xì)胞為樣品,完成了粒子富集與分離的操控實(shí)驗(yàn);通過圖像計(jì)數(shù)方法對(duì)富集效果進(jìn)行了評(píng)價(jià)。結(jié)果表明:頻率為100k Hz~10MHz時(shí),聚苯乙烯微球受負(fù)介電泳力作用電極中心或電極間距中心富集以及某確定高度平面上富集,富集后粒子個(gè)數(shù)是富集前的三倍;而酵母菌細(xì)胞在該頻率范圍內(nèi)受正介電泳力作用在電極邊緣富集,粒子個(gè)數(shù)是富集前的兩倍。利用兩種粒子受介電泳力方向不同,實(shí)現(xiàn)了兩種樣品在三維空間上分離。
[Abstract]:Dielectric electrophoretic technology is one of the key techniques to realize particle enrichment and separation in microfluidic system. Compared with other methods, such as mechanical or optical, it has the advantages of easy operation, fast response, non-contact, easy to realize miniaturization and automation, etc. It has great potential in the accurate capture, enrichment, separation and transport of some macromolecular particles and biological cells. Most of the existing microelectrophoretic chips are fabricated by Micro-Electro-Mechanical system MEMS (Micro-Electro-Mechanical system MEMS) technology, but the process equipment is expensive and the working procedure is complex, so it is not possible to realize mass production at present, which limits the popularization and application of the chip. The printed Circuit board (PCB) process has the advantages of low cost, mass production and easy packaging. It is of great significance and practical value to study the use of PCB process to process the dielectric electrophoresis chip and realize particle control. Aiming at the limitation of the resolution of PCB process, the optimization design of AC medium electrophoresis chip based on PCB process and the principle and experiment of particle enrichment and separation by using this chip are studied in this paper. It includes the following aspects: 1 analyses the principle of dielectric electrophoretic technique to control the enrichment and separation of particles. The force of neutral particles suspended in dielectric solution under the action of non-uniform electric field was analyzed, and the main influencing factors of particle movement were obtained. 2. The two-dimensional simulation model of cross finger dielectric electrophoretic chip was established. The distribution of the electric field in the chip is simulated under the condition of the resolution of the PCB process. The relationship of channel height, electrode characteristic parameters (electrode width / spacing) and voltage with the dielectric electrophoresis force was obtained. The electrode parameters of dielectric electrophoretic chip based on Laplace equation were optimized. The expression of particle suspension height, the key parameter affecting the separation of dielectric electrophoresis, was derived by Fourier series analysis, and the results were compared with the simulation results. The calculation results are verified. The effect of electrode thickness on the suspension height of particles increased with the increase of electrode thickness. A cross finger electrophoresis chip with electrode width / spacing of 100 渭 m was fabricated by PCB process. Using polystyrene microspheres and yeast cells as samples, the experiment of particle enrichment and separation was completed, and the effect of enrichment was evaluated by image counting method. The results show that when the frequency is 100k Hz~10MHz, the polystyrene microspheres are enriched by negative dielectric electrophoretic force at the electrode center or electrode spacing center and on a certain height plane, and the number of particles after enrichment is three times as much as that before enrichment. The yeast cells were enriched at the electrode edge by positive electrophoretic force in this frequency range, and the number of particles was twice as large as that before enrichment. The separation of the two samples in three dimensional space was realized by using the different directions of the two kinds of particles subjected to dielectric electrophoresis.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TN41

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本文編號(hào)：1915740