本文選題：有機(jī)電化學(xué)晶體管 + 生物傳感器　； 參考：《湖南師范大學(xué)》2016年碩士論文

【摘要】：MicroRNAs(miRNAs)是一種與生物代謝息息相關(guān)的小分子RNA。近年來,研究發(fā)現(xiàn)多種mi RNAs與腫瘤的生長、轉(zhuǎn)移等密切相關(guān),因此,mi RNAs的檢測對于疾病,尤其是腫瘤的早期診斷具有重要意義。電化學(xué)生物傳感器具有操作簡便、靈敏度高以及響應(yīng)速度快等很多優(yōu)點(diǎn),被廣泛應(yīng)用于miRNAs的檢測中。本論文制備了基于PEDOT:PSS活性層的有機(jī)電化學(xué)晶體管(Organic Electrochemical Transistors,OECTs),利用不同的修飾電極作為OECTs的柵電極,對其相關(guān)性能進(jìn)行了研究,并以此OECTs為信號(hào)放大裝置,構(gòu)建了適于miRNAs檢測的電化學(xué)生物傳感器。在最優(yōu)的實(shí)驗(yàn)條件下,所制備的有機(jī)電化學(xué)晶體管生物傳感器對miRNA24有高靈敏度、低檢測下限,具有一定的應(yīng)用前景。主要工作如下:(1)利用絲網(wǎng)印刷技術(shù)制備了碳源、漏電極,利用旋涂法制備了基于PEDOT:PSS活性層的OECTs,以HS-DNA(與mi RNA24互補(bǔ))修飾的金電極作為柵電極,構(gòu)建了基于有機(jī)電化學(xué)晶體管的mi RNA24生物傳感器。在最優(yōu)化檢測條件下,該傳感器對mi RNA24的檢測下限為20 pM;線性范圍為1 nM-200 nM;靈敏度為4.903nM-1;選擇性良好,miRNA29及mismatch miRNA24對該傳感器的響應(yīng)較小。(2)通過Au-S鍵在金電極表面固定上HS-DNA,并以此為OECTs的柵電極,構(gòu)建了基于亞鐵氰化鉀探針信號(hào)放大的有機(jī)電化學(xué)晶體管miRNA24生物傳感器,并對其性能進(jìn)行了詳細(xì)研究。在最優(yōu)化檢測條件下,該傳感器對miRNA24的檢測下限為30 fM;線性范圍為10 pM-10 nM;靈敏度為0.05989 decade-1。(3)以基于碳漿電極和Au/Ti電極的OECTs器件為模型,對OECTs器件的傳輸性能、輸出性能、穩(wěn)定性等方面進(jìn)行了詳細(xì)研究。此外,為了提高傳感器的靈敏度,我們在柵電極上電沉積納米金,構(gòu)建了基于納米金的miRNA24生物傳感器,并對該生物傳感器檢測mi RNA24的可行性進(jìn)行了初步研究。
[Abstract]:MicroRNAs (miRNAs) is a small molecule RNA. related to biological metabolism in recent years. It has been found that a variety of MI RNAs is closely related to the growth and metastasis of tumor. Therefore, the detection of MI RNAs is of great significance for the early diagnosis of diseases, especially the tumor. The electrochemical biosensor has the advantages of simple operation, high sensitivity and response. A lot of advantages, such as fast speed and so on, are widely used in the detection of miRNAs. In this paper, the Organic Electrochemical Transistors (OECTs) based on the active layer of PEDOT:PSS was prepared, and the different modified electrodes were used as the gate electrode of OECTs, and the correlation energy was studied, and OECTs was used as a signal amplification device. The electrochemical biosensor suitable for miRNAs detection is built. Under the optimal experimental conditions, the electrochemical transistor biosensor has high sensitivity and low detection limit for miRNA24. The main work is as follows: (1) the carbon source and leakage electrode have been prepared by the screen printing technology. Based on PEDOT:PSS active layer OECTs, a mi RNA24 biosensor based on electromechanical chemical transistors is constructed with a gold electrode modified with HS-DNA (complementary to MI RNA24) as a gate electrode. Under optimal detection conditions, the detection limit of the sensor to MI RNA24 is 20 pM; the linear range is 1 nM-200 nM; sensitivity is good and selectivity is good. Well, the response of miRNA29 and mismatch miRNA24 to the sensor is small. (2) the Au-S bond is fixed on the surface of the gold electrode and HS-DNA is fixed on the surface of the gold electrode, and as a OECTs gate electrode, a electromechanical chemical transistor miRNA24 biosensor based on the amplification of the potassium ferrocyanide probe signal is constructed, and its performance is studied in detail. The detection limit of the sensor to miRNA24 is 30 fM, the linear range is 10 pM-10 nM, and the sensitivity is 0.05989 decade-1. (3) based on the OECTs device of the carbon pulp electrode and Au/Ti electrode. The transmission performance, the output performance and the stability of the OECTs device are studied in detail. In addition, in order to improve the sensitivity of the sensor, I We electrodeposited nanoscale gold on the gate electrode and constructed a miRNA24 biosensor based on gold nanoparticles. The feasibility of the biosensor to detect mi RNA24 was preliminarily studied.

【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：TN32;O657.1
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本文編號(hào)：1847654