本文選題：碳化硅量子點(diǎn)　切入點(diǎn)：腐蝕法制備　出處：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：量子點(diǎn)材料由于具有獨(dú)特的光學(xué)性能在熒光標(biāo)記、成像及動態(tài)示蹤方面?zhèn)涫荜P(guān)注,成為國內(nèi)外納米生物技術(shù)領(lǐng)域的研究熱點(diǎn)。日前,隨著對鎘系量子點(diǎn)(如CdSe、CdTe、CdS等)研究的不斷深入,其生物學(xué)應(yīng)用過程中的細(xì)胞毒性問題也日趨顯現(xiàn),開發(fā)低毒甚至無毒的非Cd系量子點(diǎn)已成為業(yè)界共識,近年來,碳化硅量子點(diǎn)由于具有優(yōu)良的光學(xué)特性及生物相容性成為最具有發(fā)展前景的新型納米生物材料。基于此,本文開發(fā)出一種粒子尺寸及表面物化特性可調(diào)控的碳化硅量子點(diǎn)制備新工藝,探究了材料成型過程中微觀形貌的演變過程、各工藝參數(shù)對其光學(xué)性能的影響、量子點(diǎn)表面修飾對其特征發(fā)射光譜的影響、SiC量子點(diǎn)活體細(xì)胞引入形式及其相互作用、熒光標(biāo)記的活體細(xì)胞穩(wěn)定性及長時程示蹤等內(nèi)容,獲得如下結(jié)果：首先,采用化學(xué)腐蝕法,一步法制備出表面具有親有機(jī)物基團(tuán)的碳化硅量子點(diǎn)水相溶液,具體過程為：采用自蔓延燃燒合成的均質(zhì)β-SiC粉體材料,通過調(diào)整腐蝕劑組分及相對含量,實(shí)現(xiàn)SiC顆粒的快速腐蝕,經(jīng)降酸處理后烘干,機(jī)械研磨結(jié)合超聲波空化破碎處理,最后在超重力場中層析剪裁,獲得SiC量子點(diǎn)水相溶液。對其成型過程中微觀組織形貌演變規(guī)律進(jìn)行研究,結(jié)果表明,由于自蔓延燃燒具有反應(yīng)迅速、冷卻速度快而易形成非平衡結(jié)晶條件的特點(diǎn),使得SiC顆粒表面形成較多缺陷(如晶格畸變等),腐蝕激活能較低,因此無需通電,SiC顆粒便極易在混合腐蝕劑內(nèi)被腐蝕為多孔鏤空結(jié)構(gòu),這對量子點(diǎn)尺寸調(diào)控至關(guān)重要。其次,通過調(diào)整腐蝕劑組分及其配比,可以實(shí)現(xiàn)SiC量子點(diǎn)表面親有機(jī)物基團(tuán)的有效調(diào)控,如當(dāng)V(HF):V(HNO3):V(H2SO4)=6:1:1時,既能保證巰基的耦合穩(wěn)定性又能保證其較大的光致發(fā)光強(qiáng)度。對量子點(diǎn)光學(xué)特性的研究結(jié)果表明,當(dāng)激發(fā)光波長為340 nm時,其光致發(fā)光波長為450 nm,強(qiáng)度最大,斯托克斯位移可達(dá)110 nm,隨著激發(fā)光波長增加,發(fā)射光將發(fā)生紅移。同時,發(fā)射光波長與量子點(diǎn)尺寸密切相關(guān),相同激發(fā)光波長下,隨著直徑減小,發(fā)射光會發(fā)生藍(lán)移。最后,通過內(nèi)吞法,實(shí)現(xiàn)了出芽短梗霉、串珠鐮刀菌活體細(xì)胞SiC量子點(diǎn)穩(wěn)定熒光標(biāo)記與長時程成像示蹤；研究了根皮苷對串珠鐮刀菌生長過程的影響,對該菌侵染蘋果植株根系過程進(jìn)行動態(tài)示蹤,結(jié)果表明,根皮苷可促進(jìn)串珠鐮刀菌的分裂生長過程,串珠鐮刀菌侵染植株根部時,外表皮細(xì)胞并不存在特殊易感染部位。
[Abstract]:Quantum dot materials with unique optical properties in fluorescent imaging and dynamic tracing concern, become a hot research topic at home and abroad in the field of nano biotechnology. The day before, with the quantum of cadmium point (such as CdSe, CdTe, CdS etc.) the deepening of the study, the cytotoxicity of the biological problems in the application process is more, the development of low toxic or non-toxic and non Cd quantum dots has become the industry consensus, in recent years, SiC quantum dots as provided with optical properties and biological compatibility of excellent new nano biomaterials become most promising. Based on this, this paper developed a kind of particle size and surface physicochemical properties can be controlled the new process of preparing SiC quantum dots system, explores the evolution of microstructure of material forming process, effects of various parameters on the optical properties of the modified surface emitting quantum dot on its characteristics The influence of spectra of SiC quantum dots and their interactions in living cells form into living cells, stability and long fluorescence process tracing, the results obtained are as follows: firstly, using chemical etching method, a step surface was prepared with SiC QDs organophilically group phase solution, the specific process for using homogeneous beta -SiC powder materials synthesized by combustion, by adjusting the etchant composition and relative content, to achieve rapid corrosion of SiC particles, the reduction of acid treatment after drying, mechanical lapping with ultrasonic cavitation crushing, the super gravity field was cut, SiC quantum dots on the microstructure of the solution phase. In the forming process of morphology evolution was studied. The results showed that the self propagating combustion has the rapid reaction characteristics of non equilibrium crystallization condition of the formation of the cooling speed quickly and easily, so that the surface of SiC particles The formation of more defects (such as lattice distortion, etc.) the corrosion activation energy is low, so no electricity, SiC particles will easily be mixed in caustic corrosion of porous hollow structure, the quantum dot size regulation is vital. Secondly, by adjusting the etchant compositions can achieve effective control of surface SiC quantum dots organophilically groups, such as V (HF): V (HNO3): V (H2SO4) =6:1:1, which can guarantee the stability of coupled thiol can ensure that the photoluminescence intensity of the larger light. Study on optical properties of quantum dots. The results show that when the excitation wavelength is 340 nm. The photoluminescence wavelength is 450 nm, the maximum strength, Stokes displacement can reach 110 nm with increasing excitation wavelength, emission light will be shifted. At the same time, emission wavelength and the size of quantum dots is closely related to the same excitation wavelength, with the diameter decrease, emitting light blue shifts. After the method realized by endocytosis, Aureobasidium pullulans, Fusarium moniliforme living cells stabilized SiC QDs fluorescence labeling and long time imaging tracer; effects of phlorizin on growth of Fusarium moniliforme, dynamic tracing, the infection of Apple root process results show that phloridzin can promote the growth process of Fusarium moniliforme split, Fusarium moniliforme infection root, skin cells do not have special infection location.

【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：Q2-33;O471.1

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本文編號：1697089