本文選題：石墨烯　切入點(diǎn)：生物傳感器　出處：《山東師范大學(xué)》2017年碩士論文

【摘要】：石墨烯(Graphene)因其集優(yōu)異的熱學(xué)、力學(xué)、光學(xué)和電學(xué)性能,成為材料科學(xué)、生命科學(xué)和其他許多領(lǐng)域的研究熱點(diǎn)。生物傳感器以其在靈敏性、選擇性、準(zhǔn)確性、快速性、低成本以及操作性等方面的優(yōu)勢(shì),已成為生化分析領(lǐng)域的重要分析技術(shù)[13]。由于石墨烯其易于與生物分子通過π-π堆垛作用結(jié)合的特性,在生物傳感器設(shè)計(jì)領(lǐng)域受到極大關(guān)注。分子吸附在石墨烯表面可以調(diào)節(jié)其電荷傳輸或電荷溝道參雜,引起載流子濃度和載流子遷移率的變化�；诖�,本文研究柵極電壓調(diào)制的石墨烯場(chǎng)效應(yīng)管(FET)生物傳感器的構(gòu)建、相關(guān)檢測(cè)系統(tǒng)及其應(yīng)用。首先介紹了石墨烯獨(dú)特的結(jié)構(gòu)和性質(zhì),總結(jié)了石墨烯的制備方法。隨后介紹了生物傳感器在國內(nèi)外的研究進(jìn)展及現(xiàn)狀,生物傳感器以其在高靈敏性、選擇性、準(zhǔn)確性、快速性、低成本以及操作性等方面的優(yōu)勢(shì),已成為生化分析領(lǐng)域的重要分析技術(shù)。其次,設(shè)計(jì)制備了一種集成石墨烯場(chǎng)效應(yīng)管(IGFET),柵極采用平面金(Au)膜,而漏極和源極采用氧化銦錫(ITO)電極。平面Au柵極與石墨烯FET集成,并連至一個(gè)可調(diào)恒壓源產(chǎn)生柵極和石墨烯之間的穩(wěn)定電場(chǎng),從而調(diào)制溝道電導(dǎo)以及Ag/AgCl電極。由于石墨烯FET的溝道電導(dǎo)可以調(diào)制,那么可以將FET的等效電阻作為一個(gè)信號(hào),通過一個(gè)橋接電路和一個(gè)檢測(cè)放大器的信號(hào)獲得。本文研究了FET的等效電阻與導(dǎo)電溝道寬度、柵極和石墨烯平板之間的距離、電介質(zhì)濃度直接的關(guān)系,并利用IGFET用于ATP濃度檢測(cè)。然后,基于石墨烯的光學(xué)和電學(xué)特性,利用石墨烯研制了一種光電雙通道同時(shí)檢測(cè)溶液pH值的場(chǎng)效應(yīng)管傳感器。一方面石墨烯FET表現(xiàn)出了清晰的pH值依賴型導(dǎo)電特性,可以在源極和漏極之間產(chǎn)生電流信號(hào);另一方面在電解質(zhì)溶液中添加pH熒光探針,實(shí)現(xiàn)pH值熒光檢測(cè)。通過自主研發(fā)的一種雙通道數(shù)據(jù)采集系統(tǒng),可以同時(shí)采集電流和熒光兩種信號(hào)。結(jié)果表明,利用該雙通道數(shù)據(jù)采集系統(tǒng)獲得比單一方法更加靈敏的pH值檢測(cè)。
[Abstract]:Because of its excellent thermal, mechanical, optical and electrical properties, graphene has become a research hotspot in materials science, life sciences and many other fields. The advantages of low cost and maneuverability have become an important analytical technique in the field of biochemical analysis. [13] because graphene is easy to combine with biomolecules by 蟺-蟺 stacking, In the field of biosensor design, molecular adsorption on the surface of graphene can adjust its charge transport or charge channel impurity, resulting in changes in carrier concentration and carrier mobility. In this paper, the construction of graphene field effect tube (FET) biosensor with grid voltage modulation, the related detection system and its application are studied. Firstly, the unique structure and properties of graphene are introduced. The preparation methods of graphene were summarized, and the research progress and current situation of biosensors at home and abroad were introduced. Biosensors had the advantages of high sensitivity, selectivity, accuracy, rapidity, low cost and maneuverability. It has become an important analytical technique in the field of biochemical analysis. Secondly, a kind of integrated graphene field effect tube (IGFETT) is designed and fabricated. The gate is made of planar au film, while the drain electrode and the source electrode are made of indium tin oxide (ITO) electrode. The planar au gate is integrated with graphene FET, and the planar au gate is integrated with graphene FET. Connected to a tunable constant voltage source to generate a stable electric field between the grid and graphene, thus modulating the channel conductance and the Ag/AgCl electrode. Since the channel conductance of the graphene FET can be modulated, the equivalent resistance of the FET can be used as a signal. A bridge circuit and a detection amplifier are used to obtain the signal. The direct relationship between the equivalent resistance of FET and the width of the conductive channel, the distance between the gate and the graphene plate, and the dielectric concentration are studied. Then, based on the optical and electrical properties of graphene, A field effect tube sensor for simultaneous detection of pH value in solution by using graphene has been developed. On the one hand, graphene FET exhibits a clear pH dependent conductivity, which can generate current signals between the source and drain electrode. On the other hand, pH fluorescence probe is added to electrolyte solution to realize pH fluorescence detection. Through a dual channel data acquisition system developed by ourselves, both current and fluorescence signals can be collected simultaneously. The two-channel data acquisition system is used to obtain more sensitive pH detection than the single method.
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TN386;TP212.3

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本文編號(hào)：1693022