本文關(guān)鍵詞： 微流體 聚二甲基硅氧烷 復(fù)乳液 超聲成像　出處：《華東理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：微流體芯片是芯片實(shí)驗(yàn)室(Lab on a chip)技術(shù)的一個(gè)重要分支。經(jīng)過數(shù)十年的發(fā)展,微流體芯片已經(jīng)發(fā)展成一種具備集成化、高通量、低成本的綜合研究平臺(tái)。微流體芯片的應(yīng)用領(lǐng)域,橫跨物理、生物、醫(yī)藥分析、微機(jī)電系統(tǒng)(MEMS)等領(lǐng)域,具有廣闊的應(yīng)用前景。微流體芯片的設(shè)計(jì)與制備,一直以來是微流體技術(shù)跨領(lǐng)域應(yīng)用的研究要點(diǎn)。由于微流體的尺度問題,導(dǎo)致諸如毛細(xì)效應(yīng)、擴(kuò)散效應(yīng)等問題;同時(shí)由于MEMS加工技術(shù)的限制,對(duì)于微流道的設(shè)計(jì)和加工的研究也比較單一,目前對(duì)于微流體通道的設(shè)計(jì)和制作,都是基于流體的二維方向。研究微流體芯片的設(shè)計(jì)和制備工藝,提出創(chuàng)新、高效的微流體設(shè)計(jì)方案,對(duì)微流體技術(shù)在各個(gè)領(lǐng)域的應(yīng)用都起到促進(jìn)作用。本文對(duì)微流道的數(shù)種平面結(jié)構(gòu)設(shè)計(jì)方案進(jìn)行了對(duì)比研究,并在此基礎(chǔ)上,采用三維非平面結(jié)構(gòu)設(shè)計(jì)的方法優(yōu)化芯片結(jié)構(gòu),得到一種制作效率高、綜合成本低、生產(chǎn)過程穩(wěn)定的PDMS非平面芯片,并成功用于復(fù)乳液液滴的制備。同時(shí),本文研究了微流體芯片的制作方法,針對(duì)非平面芯片制備時(shí)鍵合困難的問題,提出改良方案。針對(duì)常規(guī)微流體裝置制作復(fù)雜的問題,本文設(shè)計(jì)了兩種不依賴于超潔凈環(huán)境和專門設(shè)備的微流體裝置。本文介紹了此兩種裝置的制備方法,并通過實(shí)驗(yàn)驗(yàn)證了裝置的可行性。最后,本文使用非平面芯片進(jìn)行了復(fù)乳液液滴和微氣泡的制備實(shí)驗(yàn)。實(shí)驗(yàn)證明了非平面芯片的實(shí)用性以及對(duì)復(fù)乳液液滴形態(tài)控制的可靠性。使用非平面芯片制備的復(fù)乳液微氣泡,其尺寸變異系數(shù)小于1.5%,具有良好的形態(tài)學(xué)特性。通過生物體內(nèi)超聲造影實(shí)驗(yàn),初步驗(yàn)證了復(fù)乳液微氣泡用于超聲造影診斷技術(shù)的可行性。
[Abstract]:Microfluidic chip is an important branch of Lab on a chip.After decades of development, microfluidic chip has developed into a kind of integrated, high-throughput microfluidic chip. The application field of microfluidic chip, covering physics, biology, medical analysis, micro electromechanical system (MEMS) and so on, has a broad application prospect, the design and manufacture of microfluidic chip, Because of the scale problem of microfluid, it leads to such problems as capillary effect, diffusion effect and so on. At the same time, because of the limitation of MEMS processing technology, At present, the design and fabrication of microfluidic channels are based on the two-dimensional direction of fluid. The highly efficient microfluidic design scheme promotes the application of microfluidic technology in all fields. In this paper, several kinds of planar structure design schemes of microfluidic channel are compared and studied, and on the basis of this, Using the method of three-dimensional non-planar structure design to optimize the chip structure, a kind of PDMS non-planar chip with high production efficiency, low synthesis cost and stable production process was obtained, and it was successfully used in the preparation of composite emulsion droplets. In this paper, the fabrication method of microfluidic chip is studied. Aiming at the difficulty of bonding in the fabrication of non-planar chip, an improved scheme is put forward. In this paper, two kinds of microfluidic devices are designed, which are not dependent on ultra-clean environment and special equipment. The preparation methods of these two devices are introduced, and the feasibility of the devices is verified by experiments. In this paper, the preparation experiments of composite emulsion droplets and microbubbles using non-planar chips are carried out. The practicability of the non-planar chips and the reliability of controlling the droplet morphology of the composite emulsion droplets are proved by the experiments. Its dimension variation coefficient is less than 1.5, and it has good morphological characteristics. The feasibility of using composite emulsion microbubbles in ultrasound diagnosis is preliminarily verified by the experiment of biologic in vivo contrast-enhanced ultrasound.
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TN492

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