無(wú)機(jī)類納米材料抗菌性研究以及對(duì)多頭絨泡菌影響的初探
發(fā)布時(shí)間：2019-05-15 16:39
【摘要】：本論文主要研究了銀納米顆粒(Ag NPs)和Ag NPs包覆氧化鋅納米顆粒(Zn O NPs)的復(fù)合納米材料(Zn O@Ag NPs)的制備及其抗菌性能和初步研究了三種無(wú)機(jī)納米顆粒對(duì)多頭絨泡菌的影響。具體內(nèi)容如下:1.以Na BH4為還原劑,Ag NO3為銀源,聚乙烯吡咯烷酮(PVP)和羧化殼聚糖(Cchitosan)為分散劑,并在制備過(guò)程中加入適量的Na OH,通過(guò)調(diào)整制備參數(shù)制備出了分別以PVP和C-chitosan為分散劑的Ag NPs分散液,二者的形狀為球形,晶體結(jié)構(gòu)類型均為FCC,粒徑分布范圍分別為14.4-36.7 nm和8-38.4 nm,均在水溶液中均勻分散。采用抑菌圈法和連續(xù)LB培養(yǎng)基稀釋法對(duì)上述兩種Ag NPs的抗菌性能進(jìn)行了評(píng)價(jià),結(jié)果表明本論文制備出的Ag NPs的抗菌性能優(yōu)異,并對(duì)酵母菌的生長(zhǎng)也有一定的抑制作用;本論文也借助SEM和激光掃描共聚焦顯微鏡(CLSM)等技術(shù)手段對(duì)Ag NPs的抗菌機(jī)制進(jìn)行了探究。Ag NPs的抗菌作用主要是造成細(xì)菌或真菌細(xì)胞內(nèi)部大量產(chǎn)生活性氧物質(zhì)(ROS)引起細(xì)菌或真菌細(xì)胞的氧化損傷的方式來(lái)實(shí)現(xiàn)的;2.以Zn O NPs為核心,以Ag(TEA)2+為銀源,以甲醛或水合肼為還原劑,采用簡(jiǎn)單的三步法制備出了Zn O@Ag NPs。結(jié)果表明,Zn O@Ag NPs表面的Ag NPs的晶體結(jié)構(gòu)類型為面心立方結(jié)構(gòu)(fcc),形狀為球形或橢球形,粒徑變化范圍為10-42 nm,Zn O@Ag NPs的包覆率也出現(xiàn)相應(yīng)的變化。采用抑菌圈法和連續(xù)LB培養(yǎng)基稀釋法對(duì)Zn O@Ag NPs的抗菌性能進(jìn)行了評(píng)價(jià)。結(jié)果表明Zn O@Ag NPs的抗菌性能優(yōu)異;而且Zn O@Ag NPs對(duì)酵母菌的生長(zhǎng)也有一定的抑制作用;Zn O@Ag NPs對(duì)金黃色葡萄球菌的抗菌性能與PVP分散的Ag NPs比較接近,但是其銀的含量?jī)H為后者的25%;其對(duì)酵母菌的MIC值為PVP-Ag NPs的1.5倍,為C-chitosan-Ag NPs的3倍,但是其銀的含量?jī)H為后兩者的25%,因此,其對(duì)酵母菌的綜合抑制作用也與PVP分散的Ag NPs相當(dāng)。本論文也借助CLSM對(duì)Ag NPs的抗菌機(jī)制進(jìn)行了探究。Zn O@Ag NPs的抗菌作用主要與其造成細(xì)菌或真菌細(xì)胞內(nèi)部大量產(chǎn)生活性氧物質(zhì)(ROS)引起細(xì)菌或真菌細(xì)胞的氧化損傷有關(guān);3.以多頭絨泡菌為作用對(duì)象,本論文初步研究了Zn O NPs、二氧化鈦納米顆粒(Ti O2 NPs)和第2章中的PVP分散的Ag NPs的毒性。Zn O NPs、Ti O2 NPs和Ag NPs對(duì)多頭絨泡菌的明顯抑制濃度分別為1750μg/m L、15000μg/m L和300μg/m L,Ag NPs對(duì)多頭絨泡菌的毒性要明顯強(qiáng)于Zn O NPs和Ti O2 NPs,Ti O2 NPs最小。在較高濃度的Zn O NPs作用下,多頭絨泡菌出現(xiàn)了 逃逸‖現(xiàn)象,Ti O2 NPs和Ag NPs卻沒(méi)有。在Zn O NPs、Ti O2 NPs和Ag NPs作用下,多頭絨泡菌的MDA產(chǎn)生均與納米顆粒的濃度存在依賴關(guān)系,隨納米顆粒的濃度的增大而先增大后減小,說(shuō)明多頭絨泡菌原質(zhì)團(tuán)內(nèi)部的氧化損傷程度越來(lái)越大,增大到一定程度時(shí),多頭絨泡菌出現(xiàn)死亡,而多頭絨泡菌出現(xiàn)死亡也導(dǎo)致MDA產(chǎn)生量下降,這為納米顆粒的ROS毒性機(jī)制進(jìn)一步提供了證據(jù)。
[Abstract]:The preparation and antibacterial properties of the composite nano-materials (Zn-O@Ag NPs) coated with silver nano-particles (Ag-NPs) and Ag-NPs-coated zinc oxide nanoparticles (Zn-NPs) were studied in this paper. The effects of the three kinds of inorganic nanoparticles on the multi-head chorionic bacteria were studied. The specific content is as follows:1. Na-BH4 is used as a reducing agent, and the Ag-NO3 is a silver source, and the polyethylene is used as a dispersing agent, and a proper amount of Na-OH is added in the preparation process, and the Ag NPs dispersion liquid with the PVP and the C-chitosan as a dispersing agent is prepared by adjusting the preparation parameters, The shape of the two is spherical, the crystal structure type is FCC, the particle size distribution range is 14.4-36.7 nm and 8-38.4 nm, respectively, and is uniformly dispersed in the aqueous solution. The antibacterial properties of the above two kinds of Ag NPs were evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results showed that the antibacterial properties of the Ag NPs prepared by the method were excellent, and the growth of the yeast was also inhibited. The antibacterial mechanism of Ag NPs was also studied by means of SEM and laser scanning confocal microscope (CLSM). The antibacterial effect of Ag NPs is mainly caused by a large amount of reactive oxygen species (ROS) in bacteria or fungal cells causing oxidative damage to bacteria or fungal cells;2. Zn-O@Ag NPs was prepared by using an Ag (TEA)2 + as a silver source and using a simple three-step method as a reducing agent with Zn O NPs as the core. The results show that the crystal structure of the Ag NPs on the surface of the Zn-O@Ag NPs is the surface-centered cubic structure (fcc), the shape is spherical or ellipsoidal, the particle size variation range is 10-42nm, and the cladding rate of the Zn-O@Ag NPs also changes correspondingly. The antibacterial performance of Zn-O@Ag NPs was evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results show that the antibacterial performance of Zn-O@Ag-NPs is excellent, and the growth of the Zn-O@Ag-NPs has a certain inhibition effect on the growth of the yeast. The antibacterial performance of the Zn-O@Ag-NPs is close to that of the Ag-NPs in which the PVP is dispersed, but the content of the silver is only 25% of the latter; The MIC value of the yeast is 1.5 times that of the PVP-Ag NPs, which is 3 times that of the C-chitosan-Ag NPs, but the content of the silver is only 25% of the latter, so that the comprehensive inhibition effect on the yeast is also equivalent to that of the PVP-dispersed Ag NPs. The antibacterial mechanism of Ag NPs was also studied by CLSM. The antibacterial action of the Zn O@Ag NPs is mainly related to the oxidative damage of bacteria or fungal cells caused by a large amount of reactive oxygen species (ROS) inside the bacterial or fungal cells. In this paper, the toxicity of Zn-O-NPs, titanium dioxide nanoparticles (TiO2 NPs) and PVP-dispersed Ag-NPs in Chapter 2 was studied in this paper. The obvious inhibitory concentration of Zn O NPs, Ti O 2 NPs and Ag NPs on the multi-headed chorionic bacteria was 1750. m u.g/ m L,15000. m u.g/ m L and 300. m u.g/ m L, respectively. The toxicity of Ag NPs to the multi-headed chorionic bacteria was significantly stronger than that of Zn O NPs and Ti O2 NPs, and the minimum of Ti O2 NPs. Under the action of higher concentration of Zn-O NPs, the phenomenon of escape was found in the multi-headed suede, and the NPs and Ag-NPs of Ti-2 were not. Under the action of Zn-O-NPs, Ti-O2 NPs and Ag-NPs, the content of MDA in the multi-headed chorionic bacteria is dependent on the concentration of the nano-particles, and then the concentration of the nano-particles is increased, and then the concentration of the nano-particles is reduced, so that the degree of oxidation damage inside the original mass of the multi-head chorionic bacteria is more and more large, When increasing to a certain extent, the occurrence of the death of the multi-headed suede, and the decrease of the production of the MDA, the occurrence of the death of the multi-headed choriococcus has further provided the evidence for the ROS toxicity mechanism of the nano-particles.
【學(xué)位授予單位】：深圳大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1
本文編號(hào)：2477636
[Abstract]:The preparation and antibacterial properties of the composite nano-materials (Zn-O@Ag NPs) coated with silver nano-particles (Ag-NPs) and Ag-NPs-coated zinc oxide nanoparticles (Zn-NPs) were studied in this paper. The effects of the three kinds of inorganic nanoparticles on the multi-head chorionic bacteria were studied. The specific content is as follows:1. Na-BH4 is used as a reducing agent, and the Ag-NO3 is a silver source, and the polyethylene is used as a dispersing agent, and a proper amount of Na-OH is added in the preparation process, and the Ag NPs dispersion liquid with the PVP and the C-chitosan as a dispersing agent is prepared by adjusting the preparation parameters, The shape of the two is spherical, the crystal structure type is FCC, the particle size distribution range is 14.4-36.7 nm and 8-38.4 nm, respectively, and is uniformly dispersed in the aqueous solution. The antibacterial properties of the above two kinds of Ag NPs were evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results showed that the antibacterial properties of the Ag NPs prepared by the method were excellent, and the growth of the yeast was also inhibited. The antibacterial mechanism of Ag NPs was also studied by means of SEM and laser scanning confocal microscope (CLSM). The antibacterial effect of Ag NPs is mainly caused by a large amount of reactive oxygen species (ROS) in bacteria or fungal cells causing oxidative damage to bacteria or fungal cells;2. Zn-O@Ag NPs was prepared by using an Ag (TEA)2 + as a silver source and using a simple three-step method as a reducing agent with Zn O NPs as the core. The results show that the crystal structure of the Ag NPs on the surface of the Zn-O@Ag NPs is the surface-centered cubic structure (fcc), the shape is spherical or ellipsoidal, the particle size variation range is 10-42nm, and the cladding rate of the Zn-O@Ag NPs also changes correspondingly. The antibacterial performance of Zn-O@Ag NPs was evaluated by the method of bacteriostatic ring and continuous LB medium dilution. The results show that the antibacterial performance of Zn-O@Ag-NPs is excellent, and the growth of the Zn-O@Ag-NPs has a certain inhibition effect on the growth of the yeast. The antibacterial performance of the Zn-O@Ag-NPs is close to that of the Ag-NPs in which the PVP is dispersed, but the content of the silver is only 25% of the latter; The MIC value of the yeast is 1.5 times that of the PVP-Ag NPs, which is 3 times that of the C-chitosan-Ag NPs, but the content of the silver is only 25% of the latter, so that the comprehensive inhibition effect on the yeast is also equivalent to that of the PVP-dispersed Ag NPs. The antibacterial mechanism of Ag NPs was also studied by CLSM. The antibacterial action of the Zn O@Ag NPs is mainly related to the oxidative damage of bacteria or fungal cells caused by a large amount of reactive oxygen species (ROS) inside the bacterial or fungal cells. In this paper, the toxicity of Zn-O-NPs, titanium dioxide nanoparticles (TiO2 NPs) and PVP-dispersed Ag-NPs in Chapter 2 was studied in this paper. The obvious inhibitory concentration of Zn O NPs, Ti O 2 NPs and Ag NPs on the multi-headed chorionic bacteria was 1750. m u.g/ m L,15000. m u.g/ m L and 300. m u.g/ m L, respectively. The toxicity of Ag NPs to the multi-headed chorionic bacteria was significantly stronger than that of Zn O NPs and Ti O2 NPs, and the minimum of Ti O2 NPs. Under the action of higher concentration of Zn-O NPs, the phenomenon of escape was found in the multi-headed suede, and the NPs and Ag-NPs of Ti-2 were not. Under the action of Zn-O-NPs, Ti-O2 NPs and Ag-NPs, the content of MDA in the multi-headed chorionic bacteria is dependent on the concentration of the nano-particles, and then the concentration of the nano-particles is increased, and then the concentration of the nano-particles is reduced, so that the degree of oxidation damage inside the original mass of the multi-head chorionic bacteria is more and more large, When increasing to a certain extent, the occurrence of the death of the multi-headed suede, and the decrease of the production of the MDA, the occurrence of the death of the multi-headed choriococcus has further provided the evidence for the ROS toxicity mechanism of the nano-particles.
【學(xué)位授予單位】：深圳大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1
【參考文獻(xiàn)】
中國(guó)期刊全文數(shù)據(jù)庫(kù) 前7條
1 史立平;李玉;;多頭絨泡菌的生活史[J];東北師大學(xué)報(bào)(自然科學(xué)版);2010年04期
2 夏和生,王琪;納米技術(shù)進(jìn)展[J];高分子材料科學(xué)與工程;2001年04期
3 鄧文雅;趙宗彬;沈琳;邱介山;;氧化鋅空心球的制備及光致發(fā)光特征[J];功能材料;2007年09期
4 楊海朋;陳仕國(guó);李春輝;陳東成;戈早川;;納米電化學(xué)生物傳感器[J];化學(xué)進(jìn)展;2009年01期
5 沈興海，高宏成;納米微粒的微乳液制備法[J];化學(xué)通報(bào);1995年11期
6 郭永，鞏雄，楊宏秀;納米微粒的制備方法及其進(jìn)展[J];化學(xué)通報(bào);1996年03期
7 袁金華;李光;陳慧珍;查河霞;宋黎軍;;納米氧化鋅對(duì)人胚肺成纖維細(xì)胞的生物毒性[J];中山大學(xué)學(xué)報(bào)(醫(yī)學(xué)科學(xué)版);2009年02期
中國(guó)碩士學(xué)位論文全文數(shù)據(jù)庫(kù) 前3條
1 沈健;納米技術(shù)進(jìn)展研究[D];中南大學(xué);2004年
2 張國(guó)山;納米科技發(fā)展研究[D];武漢科技大學(xué);2009年
3 高敏杰;銀及氧化鋅納米材料的制備及抗菌性能[D];河南大學(xué);2012年
，本文編號(hào)：2477636
本文鏈接：http://sikaile.net/kejilunwen/cailiaohuaxuelunwen/2477636.html
最近更新
教材專著
