金基殼層材料的光學(xué)性質(zhì)及負載pEGFP-C1質(zhì)粒的研究
發(fā)布時間：2018-11-23 07:47
【摘要】：納米技術(shù)的不斷發(fā)展,多功能納米材料在生物、醫(yī)學(xué)領(lǐng)域備受人們的關(guān)注,特別是利用納米材料的物化特性來研究生物體系的相互作用過程問題已成為目前的研究熱點。然而納米材料在生物醫(yī)學(xué)的應(yīng)用仍然面臨許多挑戰(zhàn)性問題。其中納米材料與生物分子相互作用的機理仍沒有全面的認(rèn)識,如何用有效手段來確定它們的結(jié)合位點等問題成為人們研究的重點。一種介電質(zhì)為核,貴金屬為殼的復(fù)合納米材料因其大的散射截面和良好生物相容性成為新的生物功能材料�；诮饸ぜ{米材料與生物分子的復(fù)合物在光的照射下會產(chǎn)生高靈敏的表面增強拉曼光譜(SERS)。通過光譜可以判斷生物分子與納米材料的作用位點,同時該材料能夠與生物分子復(fù)合后進入細胞,利用SERS進行體內(nèi)示蹤。所以金基殼層納米材料將有助于研究其與生物分子的相互作用,可以廣泛應(yīng)用于生物標(biāo)記、醫(yī)學(xué)診斷和生物組織識別、以及藥物釋放、光熱治療等生物醫(yī)學(xué)領(lǐng)域。本論文著重研究Au/Ag合金殼層材料的拉曼增強效應(yīng),并利用金基殼層材料研究其與質(zhì)粒的作用機制問題,具體內(nèi)容如下:1.通過改變金與銀源的相對比例,制備粒徑可控的以二氧化硅球為核,Au、Ag合金為殼的復(fù)合材料。以Au/Ag合金殼層材料為基底進行對巰基苯胺的拉曼檢測。通過分析拉曼光譜,發(fā)現(xiàn)Au/Ag合金殼層復(fù)合材料要比純金殼層材料的SERS活性強,是一種生物相容性好、活性高的SERS基底材料。2.為了使金殼復(fù)合納米材料能偶聯(lián)質(zhì)粒,分別利用高分子聚合物PEI、PEG修飾金殼層納米材料的表面。通過研究反應(yīng)條件對金殼層納米材料表面修飾的影響,發(fā)現(xiàn)反應(yīng)環(huán)境的p H值大于9有利于金殼層納米材料的表面修飾。最適合負載DNA的PEI-Au復(fù)合材料與質(zhì)粒p EGFP-C1相互作用后,通過凝膠阻滯實驗,獲得二者的最佳結(jié)合比例,實現(xiàn)負載質(zhì)粒。
[Abstract]:With the development of nanotechnology, multifunctional nanomaterials have attracted much attention in the field of biology and medicine. Especially, it has become a hot topic to study the interaction process of biological systems by using the physicochemical properties of nanomaterials. However, the application of nanomaterials in biomedicine still faces many challenges. The mechanism of interaction between nanomaterials and biomolecules has not been fully understood. How to determine their binding sites by effective means has become the focus of research. A composite nanomaterial with dielectric material as core and precious metal as shell has become a new biofunctional material because of its large scattering cross section and good biocompatibility. The complex based on gold shell nanomaterials and biomolecules can produce highly sensitive surface-enhanced Raman spectroscopy (SERS).) under light irradiation. The interaction sites between biomolecules and nanomaterials can be determined by spectrum. At the same time, the biomolecules can be combined with biomolecules to enter cells and trace in vivo by using SERS. Therefore, gold based shell nanomaterials will be helpful to study their interactions with biomolecules and can be widely used in biomedical fields such as biomarkers, medical diagnostics and biologic tissue recognition, drug release, photothermal therapy, and so on. In this paper, the Raman enhancement effect of Au/Ag alloy shell material is studied, and the mechanism of its interaction with plasmids is studied by using gold base shell material. The main contents are as follows: 1. By changing the relative ratio of gold and silver, a composite material with controlled particle size of silica ball as core and Au,Ag alloy as shell was prepared. The Au/Ag alloy shell material was used as the substrate for the Raman detection of p-mercaptophenylamine. By analyzing Raman spectra, it is found that Au/Ag alloy shell composite has higher SERS activity than pure gold shell material, and is a kind of SERS substrate with good biocompatibility and high activity. 2. In order to make the gold-shell composite nano-materials can be coupled with plasmids, the surface of gold-shell nanomaterials was modified by polymeric polymer PEI,PEG. By studying the effect of reaction conditions on the surface modification of gold shell nanomaterials, it is found that the pH value of the reaction environment is greater than 9 in favor of the surface modification of gold shell nanomaterials. After interaction between PEI-Au composite and plasmid p EGFP-C1 which is most suitable for loading DNA, the best proportion of the two was obtained by gel block experiment, and the loading plasmid was realized.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1
本文編號：2350823
[Abstract]:With the development of nanotechnology, multifunctional nanomaterials have attracted much attention in the field of biology and medicine. Especially, it has become a hot topic to study the interaction process of biological systems by using the physicochemical properties of nanomaterials. However, the application of nanomaterials in biomedicine still faces many challenges. The mechanism of interaction between nanomaterials and biomolecules has not been fully understood. How to determine their binding sites by effective means has become the focus of research. A composite nanomaterial with dielectric material as core and precious metal as shell has become a new biofunctional material because of its large scattering cross section and good biocompatibility. The complex based on gold shell nanomaterials and biomolecules can produce highly sensitive surface-enhanced Raman spectroscopy (SERS).) under light irradiation. The interaction sites between biomolecules and nanomaterials can be determined by spectrum. At the same time, the biomolecules can be combined with biomolecules to enter cells and trace in vivo by using SERS. Therefore, gold based shell nanomaterials will be helpful to study their interactions with biomolecules and can be widely used in biomedical fields such as biomarkers, medical diagnostics and biologic tissue recognition, drug release, photothermal therapy, and so on. In this paper, the Raman enhancement effect of Au/Ag alloy shell material is studied, and the mechanism of its interaction with plasmids is studied by using gold base shell material. The main contents are as follows: 1. By changing the relative ratio of gold and silver, a composite material with controlled particle size of silica ball as core and Au,Ag alloy as shell was prepared. The Au/Ag alloy shell material was used as the substrate for the Raman detection of p-mercaptophenylamine. By analyzing Raman spectra, it is found that Au/Ag alloy shell composite has higher SERS activity than pure gold shell material, and is a kind of SERS substrate with good biocompatibility and high activity. 2. In order to make the gold-shell composite nano-materials can be coupled with plasmids, the surface of gold-shell nanomaterials was modified by polymeric polymer PEI,PEG. By studying the effect of reaction conditions on the surface modification of gold shell nanomaterials, it is found that the pH value of the reaction environment is greater than 9 in favor of the surface modification of gold shell nanomaterials. After interaction between PEI-Au composite and plasmid p EGFP-C1 which is most suitable for loading DNA, the best proportion of the two was obtained by gel block experiment, and the loading plasmid was realized.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1
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