【摘要】：納米技術的不斷發(fā)展,多功能納米材料在生物、醫(yī)學領域備受人們的關注,特別是利用納米材料的物化特性來研究生物體系的相互作用過程問題已成為目前的研究熱點。然而納米材料在生物醫(yī)學的應用仍然面臨許多挑戰(zhàn)性問題。其中納米材料與生物分子相互作用的機理仍沒有全面的認識,如何用有效手段來確定它們的結合位點等問題成為人們研究的重點。一種介電質為核,貴金屬為殼的復合納米材料因其大的散射截面和良好生物相容性成為新的生物功能材料�；诮饸ぜ{米材料與生物分子的復合物在光的照射下會產生高靈敏的表面增強拉曼光譜(SERS)。通過光譜可以判斷生物分子與納米材料的作用位點,同時該材料能夠與生物分子復合后進入細胞,利用SERS進行體內示蹤。所以金基殼層納米材料將有助于研究其與生物分子的相互作用,可以廣泛應用于生物標記、醫(yī)學診斷和生物組織識別、以及藥物釋放、光熱治療等生物醫(yī)學領域。本論文著重研究Au/Ag合金殼層材料的拉曼增強效應,并利用金基殼層材料研究其與質粒的作用機制問題,具體內容如下:1.通過改變金與銀源的相對比例,制備粒徑可控的以二氧化硅球為核,Au、Ag合金為殼的復合材料。以Au/Ag合金殼層材料為基底進行對巰基苯胺的拉曼檢測。通過分析拉曼光譜,發(fā)現(xiàn)Au/Ag合金殼層復合材料要比純金殼層材料的SERS活性強,是一種生物相容性好、活性高的SERS基底材料。2.為了使金殼復合納米材料能偶聯(lián)質粒,分別利用高分子聚合物PEI、PEG修飾金殼層納米材料的表面。通過研究反應條件對金殼層納米材料表面修飾的影響,發(fā)現(xiàn)反應環(huán)境的p H值大于9有利于金殼層納米材料的表面修飾。最適合負載DNA的PEI-Au復合材料與質粒p EGFP-C1相互作用后,通過凝膠阻滯實驗,獲得二者的最佳結合比例,實現(xiàn)負載質粒。
[Abstract]:With the development of nanotechnology, multifunctional nanomaterials have attracted much attention in the field of biology and medicine. Especially, it has become a hot topic to study the interaction process of biological systems by using the physicochemical properties of nanomaterials. However, the application of nanomaterials in biomedicine still faces many challenges. The mechanism of interaction between nanomaterials and biomolecules has not been fully understood. How to determine their binding sites by effective means has become the focus of research. A composite nanomaterial with dielectric material as core and precious metal as shell has become a new biofunctional material because of its large scattering cross section and good biocompatibility. The complex based on gold shell nanomaterials and biomolecules can produce highly sensitive surface-enhanced Raman spectroscopy (SERS).) under light irradiation. The interaction sites between biomolecules and nanomaterials can be determined by spectrum. At the same time, the biomolecules can be combined with biomolecules to enter cells and trace in vivo by using SERS. Therefore, gold based shell nanomaterials will be helpful to study their interactions with biomolecules and can be widely used in biomedical fields such as biomarkers, medical diagnostics and biologic tissue recognition, drug release, photothermal therapy, and so on. In this paper, the Raman enhancement effect of Au/Ag alloy shell material is studied, and the mechanism of its interaction with plasmids is studied by using gold base shell material. The main contents are as follows: 1. By changing the relative ratio of gold and silver, a composite material with controlled particle size of silica ball as core and Au,Ag alloy as shell was prepared. The Au/Ag alloy shell material was used as the substrate for the Raman detection of p-mercaptophenylamine. By analyzing Raman spectra, it is found that Au/Ag alloy shell composite has higher SERS activity than pure gold shell material, and is a kind of SERS substrate with good biocompatibility and high activity. 2. In order to make the gold-shell composite nano-materials can be coupled with plasmids, the surface of gold-shell nanomaterials was modified by polymeric polymer PEI,PEG. By studying the effect of reaction conditions on the surface modification of gold shell nanomaterials, it is found that the pH value of the reaction environment is greater than 9 in favor of the surface modification of gold shell nanomaterials. After interaction between PEI-Au composite and plasmid p EGFP-C1 which is most suitable for loading DNA, the best proportion of the two was obtained by gel block experiment, and the loading plasmid was realized.
【學位授予單位】：東北師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1

【參考文獻】

相關期刊論文 前6條

1 周偉;張維;王程;趙子春;王延濤;牛文成;岳釗;劉國華;;貴金屬納米顆粒LSPR現(xiàn)象研究[J];傳感技術學報;2010年05期

2 李曉偉,鄭軍偉,周耀國,季媛,莊嚴,陸天虹;苯硫酚及其衍生物在銀電極表面的吸附取向[J];分析化學;2003年11期

3 季淑莉,司民真,苗潤才;納米銀粒子的量子尺寸效應[J];光子學報;1999年01期

4 陳李清;殷一丁;;核-殼結構雙金屬納米顆粒的表面增強拉曼散射的理論研究[J];蘇州大學學報(自然科學版);2012年02期

5 張立德,牟季美;物理學與新型(功能)材料專題系列介紹(Ⅲ) 開拓原子和物質的中間領域──納米微粒與納米固體[J];物理;1992年03期

6 張念椿;高燕紅;劉應亮;;納米SiO_2/Au復合粒子的制備及其性質表征[J];稀有金屬;2009年06期

相關博士學位論文 前1條

1 李菊梅;聚合物/納米銀復合微球的制備、SERS性能及其應用研究[D];復旦大學;2012年

相關碩士學位論文 前1條

1 凌麗;Ag_核Au_殼金屬納米線的合成及表面增強拉曼光譜研究[D];蘇州大學;2007年

，

本文編號：2350822