【摘要】：由于中晚期的癌癥治療難度很大,而且復(fù)發(fā)率極高。因此癌癥的治療需要盡量做到早發(fā)現(xiàn)早診斷早治療。而早期腫瘤的檢測(cè)要求醫(yī)療檢測(cè)手段具有無(wú)創(chuàng)、需對(duì)病人身體各臟器各角度各平面成像,且要求有高清晰度的成像效果以實(shí)現(xiàn)對(duì)病灶部位進(jìn)行準(zhǔn)確的定位等特點(diǎn),磁共振(MRI)成像技術(shù)能很好滿足這些臨床檢查要求。因此這些特點(diǎn)使得MRI成像技術(shù)在腫瘤的早期診斷具有很大的價(jià)值。超順磁性氧化鐵顆粒(SPIO)作為最具潛力的釓(Gd)的順磁性對(duì)比劑替代品,一直是MRI對(duì)比劑的熱門選擇。而由于原本上市的SPIO類型的T2對(duì)比劑AMI-25及SHU-555A相繼退出中國(guó)市場(chǎng),因此國(guó)內(nèi)目前SPIO類型的T2類核磁對(duì)比劑幾乎處于空白狀態(tài),研究SPIO類型的MRI對(duì)比劑在臨床上具有廣闊的應(yīng)用前景。本文的目的是建立一種具有臨床MRI成像應(yīng)用潛力的水溶性SPIO納米顆粒,并通過(guò)放大合成規(guī)模,為其臨床應(yīng)用提供理論和技術(shù)支持。本文主要研究結(jié)果有:1)通過(guò)小劑量的油相和水相合成方法,獲得了水溶性的SPIO納米顆粒,具有超順磁性且分散性良好。2)將這一合成反應(yīng)方法進(jìn)一步放大合成規(guī)模,通過(guò)對(duì)放大過(guò)程的研究及放大過(guò)程中的參數(shù)控制,實(shí)現(xiàn)了放大規(guī)模的最佳合成工藝確定,為提高SPIO納米顆粒的臨床應(yīng)用性提供了基礎(chǔ)。3)將放大合成所得的產(chǎn)物用于小鼠肝硬化和裸鼠皮下瘤模型的MRI成像造影,成像結(jié)果顯示合成出的水溶性SPIO納米顆粒具有顯著提高成像分辨率的功能,為癌癥早期的細(xì)微病變檢出提供了可能性。
[Abstract]:Because of advanced cancer treatment is very difficult, and the recurrence rate is extremely high. Therefore, the treatment of cancer needs to be early detection, early diagnosis and early treatment. The detection of early tumor requires noninvasive medical examination, imaging of every angle and plane of each organ of the patient, and high definition imaging effect in order to realize the accurate location of the lesion, and so on. Magnetic resonance (MRI) imaging can well meet these clinical requirements. Therefore, MRI imaging has great value in the early diagnosis of tumor. Superparamagnetic iron oxide (SPIO) particles, as the most promising paramagnetic contrast agent for gadolinium (Gd), have always been the hot choice of MRI contrast agents. However, AMI-25 and SHU-555A, the T2 contrast agents of SPIO type, which were originally listed on the market, have been withdrawn from the Chinese market one after another, so at present, the T2 type nuclear magnetic contrast agents of SPIO type in China are almost blank. The study of SPIO type MRI contrast agent has a broad prospect in clinical application. The aim of this paper is to establish a kind of water-soluble SPIO nanoparticles with the potential of clinical MRI imaging and to provide theoretical and technical support for its clinical application by enlarging the scale of synthesis. The main results are as follows: 1) Water-Soluble SPIO nanoparticles were obtained by low dose oil phase and water phase synthesis methods, with superparamagnetic and good dispersibility. 2) the synthesis method was further scaled up. Through the study of the amplification process and the parameter control in the amplification process, the optimal synthesis process of the large scale is determined. It provides a basis for improving the clinical application of SPIO nanoparticles. 3) the amplified products are used in MRI imaging of liver cirrhosis and subcutaneous tumor models in nude mice. The imaging results show that the synthesized water-soluble SPIO nanoparticles can significantly improve the imaging resolution and provide the possibility for the detection of small lesions in the early stage of cancer.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.7;TB383.1

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