【摘要】：石墨烯機械強度高、導(dǎo)電性好、比表面積大,作為電極修飾材料被廣泛用于電化學(xué)生物傳感器研究領(lǐng)域。本論文利用水溶性陽離子型鈷酞菁非共價修飾石墨烯得到的納米復(fù)合材料具有良好的分散性,易于成膜,將其用于電極表面修飾,對其電化學(xué)催化性能進(jìn)行了研究。具體內(nèi)容如下:1.首先利用靜電和π-π吸附作用制備了陽離子型鈷酞菁修飾的石墨烯納米復(fù)合材料(ImCoPc/RGO)。采用紫外-可見吸收光譜、紅外吸收光譜、掃描電子顯微鏡(SEM)等技術(shù)對得到的納米復(fù)合材料結(jié)構(gòu)及相貌進(jìn)行了表征。然后以滴涂法構(gòu)筑了 ImCoPc/RGO/GCE修飾電極,利用循環(huán)伏安法(CV)和差示脈沖伏安法(DPV)考察了該修飾電極對生物分子多巴胺(DA )以及NaNO_2的電化學(xué)檢測性能。結(jié)果表明,ImCoPc/RGO/GCE修飾電極可以用于多巴胺檢測,最佳pH=7.0 ,檢測范圍為10 ～ 800 μM ,檢測靈敏度為83μA·mM~(-1),檢出限為0.057 μM。該修飾電極催化多巴胺氧化反應(yīng)屬于表面控制過程,且抗壞血酸存在下可以實現(xiàn)對多巴胺的選擇性檢測。對NaNO2檢測范圍為3 ～120 μM ,靈敏度為28.16 μA·M~(-1),檢出限為0.005 μM ,修飾電極催化NaNO2氧化反應(yīng)屬于擴(kuò)散控制過程。此外,所制備的修飾電極在測試中均呈現(xiàn)出良好的穩(wěn)定性和重現(xiàn)性。2.利用層層自組裝技術(shù)在玻碳電極表面修飾了多層復(fù)合薄膜(ImCOPc/RGO)n ( n=1～6),利用循環(huán)伏安法和阻抗測定進(jìn)行了表征。并將所得電極用于水溶液中亞硝酸鹽檢測。結(jié)果表明,復(fù)合薄膜n=4時電極催化性能最佳,對NaNO2的檢測范圍為0.1～1.4 mM ,靈敏度為2.787 μA·M~(-1),檢出限15 μM,修飾電極催化NaNO2氧化反應(yīng)屬于擴(kuò)散控制過程,且呈現(xiàn)出良好的穩(wěn)定性和重現(xiàn)性。
[Abstract]:Graphene is widely used in electrochemical biosensors because of its high mechanical strength, good electrical conductivity and large specific surface area. In this paper, the nano-composites prepared by non-covalent modification of graphene with water-soluble cationic cobalt phthalocyanine have good dispersion and are easy to form films. The nano-composites are used to modify the electrode surface and their electrochemical catalytic properties are studied. The specific contents are as follows: 1. Firstly, cationic cobalt phthalocyanine modified graphene nanocomposites (ImCoPc/RGO) were prepared by electrostatic and 蟺-蟺 adsorption. The structure and appearance of the nanocomposites were characterized by UV-Vis absorption spectra, infrared absorption spectra and scanning electron microscopy (SEM) (SEM). Then the ImCoPc/RGO/GCE modified electrode was constructed by drop coating method. The electrochemical detection properties of the modified electrode for dopamine (DA) and NaNO_2 were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results showed that ImCoPc/RGO/GCE modified electrode could be used for the detection of dopamine. The optimal detection range was 10 ~ 800 渭 M, the detection sensitivity was 83 渭 A mM~ (-1) and the detection limit was 0.057 渭 M. The oxidation of dopamine catalyzed by the modified electrode is a surface controlled process, and the selective detection of dopamine can be achieved in the presence of ascorbic acid. The detection range of NaNO2 is 3 ~ 120 渭 M, the sensitivity is 28.16 渭 A M ~ (-1), the detection limit is 0.005 渭 M. the oxidation of NaNO2 catalyzed by modified electrode is a diffusion control process. In addition, the modified electrodes showed good stability and reproducibility. 2. 2. The multilayer composite film (ImCOPc/RGO) n (ng 1 + 6) was modified on the surface of glassy carbon electrode by layer self-assembly technique and characterized by cyclic voltammetry and impedance measurement. The electrode was used to detect nitrite in aqueous solution. The results show that the electrode has the best catalytic performance when the composite film n = 4. The detection range of NaNO2 is 0.1 mM, the sensitivity is 2.787 渭 A M ~ (-1), the detection limit is 15 渭 M. The oxidation of NaNO2 catalyzed by modified electrode belongs to the diffusion control process and has good stability and reproducibility.
【學(xué)位授予單位】：遼寧大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB332;O657.1

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