本文選題：生物傳感器 + 腫瘤標(biāo)記物��； 參考：《青島科技大學(xué)》2017年碩士論文

【摘要】：電化學(xué)傳感器由于其潛在的高靈敏性、易于微型化、簡(jiǎn)便快捷、花費(fèi)低等優(yōu)點(diǎn)而受到人們廣泛的關(guān)注,被廣泛應(yīng)用于生物醫(yī)藥、環(huán)境分析、生化分析等方面。但是,由于修飾材料對(duì)于電極的鈍化作用以及蛋白質(zhì)等生物大分子的非特異性吸附,使得生物傳感器在實(shí)際樣品檢測(cè)方面的應(yīng)用仍是一項(xiàng)巨大的挑戰(zhàn)。為了解決上述兩個(gè)問(wèn)題,本研究工作成功制備了兩種具有高靈敏性的電化學(xué)傳感器。(1)首先,通過(guò)原位電聚合的方法成功制備了聚三,四-乙烯二氧噻吩(PEDOT)/聚并苯(PAS)納米復(fù)合材料;得到的PEDOT/PAS納米復(fù)合材料具有較大的比表面積和較多的活性位點(diǎn),對(duì)于亞硝酸鹽的催化氧化呈現(xiàn)較高的催化活性;在最優(yōu)試驗(yàn)條件下,對(duì)于亞硝酸鈉的檢測(cè)范圍為0.3μM到6.6 mM,最低檢測(cè)限約為98 nM(S/N=3)。(2)隨后,在基于親水性材料聚多巴胺(pDA)、單寧酸(TA)、四臂聚乙二醇(PEG)基礎(chǔ)上構(gòu)建的抗蛋白非特異性吸附界面用于乳腺癌易感基因(BRCA1)的檢測(cè),線性范圍為0.1 fM到10 pM,最低檢測(cè)限約為0.05 fM(S/N=3)。而且,這兩種傳感器均可用于復(fù)雜環(huán)境中對(duì)于目標(biāo)物的定量檢測(cè),并具有良好的穩(wěn)定性和重現(xiàn)性。
[Abstract]:Electrochemical sensors are widely used in biomedicine, environmental analysis, biochemical analysis and so on because of their potential high sensitivity, easy miniaturization, simplicity and low cost. However, due to the passivation of modified materials to the electrode and the non-specific adsorption of biomolecules such as proteins, the application of biosensors in the field of sample detection is still a huge challenge. In order to solve the above two problems, two kinds of electrochemical sensors with high sensitivity have been successfully prepared in this work. (1) first of all, three kinds of electrochemical sensors have been successfully prepared by in situ electropolymerization. Tetraethylenedioxythiophene (PEDOT) / polyazobenzene (pas) nanocomposites have larger specific surface area and more active sites and exhibit higher catalytic activity for nitrite oxidation. Under the optimum test conditions, the detection range of sodium nitrite is from 0.3 渭 M to 6.6 mm, and the minimum detection limit is about 98 nm (S / N 3). (2). The nonspecific adsorption interface based on hydrophilic materials such as polydopamine (PDA), tannic acid (TA) and four arm polyethylene glycol (PEG) was used to detect breast cancer susceptibility gene (BRCA1). The linear range was from 0.1 FM to 10 pm, and the minimum detection limit was about 0.05 FM (S / N 3). Moreover, these two kinds of sensors can be used for quantitative detection of target in complex environments, and have good stability and reproducibility.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TB33;O657.1

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