發(fā)布時間：2018-07-05 15:54

  本文選題：連續(xù)熱注射法 + 近紅外光敏藥物��； 參考：《合肥工業(yè)大學》2017年碩士論文

【摘要】：近紅外光響應納米藥物的制備與應用是化學、生物、材料、醫(yī)學等領域的研究熱點。通過將上轉換熒光材料與不同性質的材料合理地復合在一起,利用材料之間的協(xié)同效應,制備的納米藥物可以在納米尺度范圍內(nèi)對腫瘤進行監(jiān)控,分析以及治療。但現(xiàn)有的合成近紅外光響應的上轉換熒光材料需要多次離心沉淀、水洗滌、重新分散晶種等一系列復雜操作。針對此問題,本論文發(fā)展了一種方便、低毒性的連續(xù)熱注射法,此合成方法是在十八烯和油酸的混合溶液里加入的定量NH_4F和NaOH,將反應溶液升到高溫后,間隔一定時間,逐次注射鑭系離子的油酸復合物,在高溫下成核生長,在反應體系的比表面能要求最小的情況下,快速生成上轉換納米晶體。利用此方法,我們制備了近紅外光響應的納米藥物,具體如下:(1)通過連續(xù)熱注射法,首先制備了超小的CaF_2:Yb,Er近紅外光響應的熒光納米顆粒(10 nm)。通過透射電子顯微鏡、X-射線衍射儀、X-射線能譜儀、熒光光譜儀、熒光光譜儀等表征手段,發(fā)現(xiàn)合成過程中Na~+的濃度能改善CaF_2:Yb,Er的結晶性、熒光強度和形貌,并發(fā)現(xiàn)連續(xù)熱注射法能有效提高的上轉換納米顆粒的熒光強度,且可有效調(diào)控上轉換納米顆粒的尺寸大小,證明了連續(xù)熱注射法是一種簡單、高效、通用的合成上轉換納米顆粒的方法。(2)在連續(xù)熱注射法基礎上,通過層層自組裝合成了近紅外光敏藥物NaYF_4:Yb,Tm@NaYF_4(UCNPs)@mSiO_2/CdS。通過透射電子顯微鏡發(fā)現(xiàn)所制備的UCNPs、CdS和介孔SiO_2的尺寸分別為50 nm、5 nm和10 nm。X-射線能譜儀和氮氣吸附脫附等表征手段發(fā)現(xiàn)UCNPs@mSiO_2/CdS具有良好的結晶性和巨大的比表面積(760 m2g-1)。紫外可見光吸收光譜和熒光光譜發(fā)現(xiàn)UCNPs的上轉換熒光(380、451和470 nm)被半導體CdS通過熒光能量共振轉移過程淬滅。通過活性自由基探針對苯二甲酸發(fā)現(xiàn)在近紅外光照射下,UCNPs會激發(fā)CdS產(chǎn)生羥基自由基(·OH)。光降解羅丹明B實驗表明UCNPs@mSiO_2/CdS在近紅外光具有高效的產(chǎn)生活性自由基的效率。同時,在980 nm近紅外光照射下,將光敏藥物UCNPs@mSiO_2/CdS與Hela癌細胞一起培養(yǎng)24 h,進行光動力治療研究,實驗結果表明所制備的光敏藥物系統(tǒng)UCNPs@mSiO_2/CdS有良好光動力治療效果。
[Abstract]:The preparation and application of NIR-responsive nanopharmaceuticals is a hotspot in chemistry, biology, materials, medicine and so on. By combining the up-conversion fluorescent materials with different materials reasonably, and utilizing the synergistic effect between the materials, the prepared nanopharmaceuticals can monitor, analyze and treat the tumors in the nanometer scale. However, the existing up-conversion fluorescent materials with near-infrared light response require a series of complex operations, such as centrifugation, water washing and re-dispersing of seeds. In order to solve this problem, a convenient and low toxicity continuous thermal injection method was developed in this paper. The synthetic method was to add quantitative NH _ 4F and NaOH in the mixed solution of octadecene and oleic acid, and to raise the reaction solution to high temperature at intervals of a certain time. The oleic acid complexes of lanthanide ions were injected one by one and nucleated at high temperature. Under the condition that the specific surface energy of the reaction system was minimal, the up-conversion nanocrystalline was formed rapidly. Using this method, we have prepared near-infrared photo-responsive nanopharmaceuticals as follows: (1) by continuous thermal injection, ultrasmall caf _ 2: Yb ~ (+) er fluorescent nanoparticles (10 nm).) have been prepared. By means of transmission electron microscope (TEM), X ray diffractometer, fluorescence spectrometer and so on, it was found that the concentration of Na ~ in the synthesis process could improve the crystallinity, fluorescence intensity and morphology of CaF2: YbPe er. It is also found that continuous thermal injection can effectively improve the fluorescence intensity of up-converted nanoparticles and can effectively control the size of up-converted nanoparticles, which proves that continuous thermal injection is a simple and efficient method. (2) on the basis of continuous thermal injection method, the near-infrared Guang Min drug NaYF4: YbChe TmPO-na-YF4 (UCNPs) @ mSiO2pCdSs was synthesized by layer self-assembly. By transmission electron microscopy, the size of the prepared UCNPsmon-CdS and mesoporous SiO-2 were 50 nm and 10 nm.X- ray spectrometer and nitrogen adsorption and desorption, respectively. It was found that UCNPssmSiO2 / CDs has good crystallinity and huge specific surface area (760 m2g-1). UCNPs' up-conversion fluorescence (380451 nm and 470nm) was quenched by semiconductor CDs via fluorescence energy resonance transfer process. By using the active free radical probe terephthalic acid, it was found that UCNPs stimulated CDs to produce hydroxyl radical (OH) under near-infrared irradiation. The photodegradation of Rhodamine B shows that UCNPs@ mSiOs has a high efficiency in producing active free radicals in near infrared light. At the same time, under the irradiation of 980nm near infrared light, the Guang Min drug UCNPs@ mSiO-1 / CDs was cultured with Hela cancer cells for 24 h, and the photodynamic therapy was studied. The experimental results showed that the prepared Guang Min drug system UCNPsr mSiOs 2% CDs had a good photodynamic therapy effect.
【學位授予單位】：合肥工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TQ460.1;TB383.1

【參考文獻】

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1 于洋洋;于美燕;張s，

本文編號：2100786