本文選題：稀土納米粒子 + 上轉(zhuǎn)換熒光��； 參考：《南京師范大學(xué)》2015年碩士論文

【摘要】：積極地尋找性能優(yōu)異的光致化學(xué)發(fā)光新材料,成為研發(fā)基于光致化學(xué)發(fā)光技術(shù)的高靈敏生物傳感器和高清晰生物成像的關(guān)鍵。本論文以稀土上轉(zhuǎn)換發(fā)光納米材料和碳量子點為研究對象,對這兩類材料的制備新技術(shù)及生物醫(yī)學(xué)應(yīng)用進(jìn)行了探索,具體研究結(jié)果如下：(1)要實現(xiàn)對生物分子的高靈敏檢測,就要使稀土上轉(zhuǎn)換發(fā)光納米材料具備良好的水溶性、生物相容性、高的熒光發(fā)射量子產(chǎn)率和多色光發(fā)射等多功能化特性。本論文以氨基酸作為有機(jī)包裹劑,合成了由30nm左右大小稀土上轉(zhuǎn)換納米粒子自組裝形成的稻草束狀超結(jié)構(gòu)YF3:Yb3+, Er34顆粒,探討了氨基酸以及氟源在形貌形成過程中的作用；此外,實驗當(dāng)中通過摻雜過渡金屬銅元素的方法,在保持稻草束狀結(jié)構(gòu)基本不變的前提下,得到了銅摻雜的六方β-NaYF4: Yb3+, Er3+上轉(zhuǎn)換熒光納米粒子,并且發(fā)現(xiàn)隨著銅元素?fù)诫s比例的提高,稀土上轉(zhuǎn)換熒光納米材料的熒光強(qiáng)度也隨之增強(qiáng),當(dāng)摻雜比例為40%時,得到最大的熒光強(qiáng)度；將上述制備得到的銅摻雜NaYF4:Yb3+.上轉(zhuǎn)換熒光納米粒子應(yīng)用到谷胱甘肽的檢測當(dāng)中,達(dá)到了40 nM的檢測限。(2)以不同氨基酸為碳源,通過水熱法制備了具有良好水溶性和較高熒光量子產(chǎn)率的碳量子點。對其進(jìn)行了X射線衍射表征和拉曼光譜表征,確認(rèn)得到的碳量子點主要成分為無定形碳。通過XPS表征數(shù)據(jù)和傅里葉紅外表征數(shù)據(jù),進(jìn)一步得知其中碳原子和氮原子的成鍵方式。當(dāng)使用不同的激發(fā)光對碳量子點溶液進(jìn)行激發(fā)之后,其發(fā)射峰的位置會隨著激發(fā)光位置的改變而改變,即具有" Excitation-Dependent"性質(zhì)。在使用396nm光源激發(fā)下,可得到碳量子點熒光的最強(qiáng)值。細(xì)胞毒性檢測試驗表明該類碳量子點細(xì)胞毒性較低,與Hela細(xì)胞培養(yǎng)24h后,使用相關(guān)激發(fā)光源進(jìn)行激發(fā),發(fā)現(xiàn)細(xì)胞呈現(xiàn)較好的形態(tài),并且能夠發(fā)出綠色熒光,這為該類碳量子點未來應(yīng)用于其他生物成像及熒光防偽領(lǐng)域提供了可能。
[Abstract]:Actively looking for new photochemiluminescence materials with excellent performance has become the key to the development of high sensitivity biosensor and high resolution biometric imaging based on photoluminescence technology. In this paper, the upconversion luminescent nanomaterials and carbon quantum dots (QDs) of rare earths are taken as the research objects, and the preparation technology and biomedical application of these two kinds of materials are explored. The specific results are as follows: 1) High sensitive detection of biomolecules should be realized. It is necessary to make rare earth up-conversion luminescent nanomaterials with good water-solubility, biocompatibility, high fluorescence emission quantum yield and multi-color emission. In this paper, the rice straw bunchy superstructure YF3: Yb3, Er34 particles were synthesized by using amino acids as organic encapsulating agent, and the effects of amino acids and fluorine sources on morphology formation were discussed. In the experiment, the copper doped hexagonal 尾 -NaYF4: Yb3, Er3 up-conversion fluorescent nanoparticles were obtained by doping the transition metal copper and keeping the straw beam structure basically unchanged, and it was found that with the increase of the doping ratio of copper, The fluorescence intensity of rare earth up-conversion fluorescent nanomaterials also increased, and the maximum fluorescence intensity was obtained when the doping ratio was 40%, and the copper doped NaYF _ 4: Yb _ 3 was obtained. Up-conversion fluorescent nanoparticles were applied to the detection of glutathione, reaching the detection limit of 40 nm. (2) carbon quantum dots with good water solubility and high fluorescence quantum yield were prepared by hydrothermal method using different amino acids as carbon source. X-ray diffraction and Raman spectroscopy were used to characterize the QDs. The main components of the QDs were identified as amorphous carbon. The bonding modes of carbon and nitrogen atoms were further obtained by XPS and Fourier transform infrared spectroscopy. When different excitation light is used to excite the carbon quantum dot solution, the position of the emission peak will change with the change of the excited light position, that is, it has the property of "Excitation-Dependent". The strongest fluorescence value of carbon quantum dots can be obtained when excited by 396nm light source. The cytotoxicity test showed that the cytotoxicity of this kind of QDs was low. After cultured with Hela cells for 24 hours, the cells were stimulated with the related excitation light source, and the cells were found to be in good shape and could emit green fluorescence. This provides the possibility for the future application of this kind of carbon quantum dots in other biological imaging and fluorescence anti-counterfeiting fields.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：O657.3;TB383.1

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