本文選題：多糖 + 手性識(shí)別　； 參考：《常州大學(xué)》2017年碩士論文

【摘要】：構(gòu)建馬鈴薯淀粉(PS)手性平臺(tái)并將其應(yīng)用于電化學(xué)識(shí)別色氨酸(Trp)對(duì)映體。由于PS對(duì)D-Trp具有更大的親和力,因此PS更傾向于與D-Trp通過(guò)主客體相互作用而結(jié)合。當(dāng)L-Trp和D-Trp分別與PS修飾電極表面接觸時(shí),L-Trp更容易通過(guò)PS的左手雙螺旋結(jié)構(gòu)而在電極表面被氧化。因此,PS修飾電極可以用于識(shí)別Trp對(duì)映體。水接觸角實(shí)驗(yàn)首次被用于證明PS與Trp對(duì)映體之間具有不同的親和力。同時(shí),PS修飾電極對(duì)于Trp對(duì)映體的識(shí)別具有溫度敏感性,這是由于PS與客體分子(Trp)之間形成的氫鍵的強(qiáng)弱受溫度的影響。值得注意的是,在PS的主鏈上引入羥丙基基團(tuán)后(H-PS),H-PS修飾電極對(duì)Trp對(duì)映體的識(shí)別效率明顯降低,這可能是由于主客體反應(yīng)過(guò)程中存在額外的空間位阻。羧甲基纖維素鈉(NaCMC)可通過(guò)鏈上的羧基和殼聚糖(CS)鏈上的氨基發(fā)生酰胺化反應(yīng),從而可形成CMC-CS復(fù)合材料。與NaCMC和CS相比,當(dāng)NaCMC和CS的質(zhì)量比為1:1時(shí)生成的復(fù)合材料(CMC-CS(1:1))展現(xiàn)出三維多孔網(wǎng)絡(luò)狀結(jié)構(gòu),由于大量的極性官能團(tuán)暴露在CMC-CS(1:1)三維多孔網(wǎng)絡(luò)狀結(jié)構(gòu)以外,從而顯著提高了CMC-CS(1:1)的親水性。本章利用CMC-CS(1:1)復(fù)合材料構(gòu)建了手性界面并將其應(yīng)用于電化學(xué)識(shí)別Trp對(duì)映體。由于CMC-CS(1:1)高度親水的特征以及在與Trp對(duì)映體形成氫鍵的過(guò)程中空間位阻不同,CMC-CS(1:1)手性界面對(duì)Trp對(duì)映體具有良好的選擇性。同時(shí),我們還對(duì)復(fù)合材料中NaCMC與CS的質(zhì)量比和制備方式進(jìn)行了優(yōu)化。結(jié)果表明,CMC-CS(1:1)手性界面可以準(zhǔn)確測(cè)定D-Trp在外消旋體溶液中的含量。在馬鈴薯淀粉凝膠化的過(guò)程中,將硫代蘋果酸(MSA)分子引入到馬鈴薯淀粉的大分子鏈上,從而破壞了馬鈴薯淀粉鏈與鏈之間的固有氫鍵。通過(guò)傅里葉變換紅外驗(yàn)證了這一過(guò)程。將MSA改性的馬鈴薯淀粉經(jīng)過(guò)冷凍干燥后可以得到MSA改性的多孔淀粉干凝膠(PSX/MSA)。最后,將PSX/MSA應(yīng)用于去除具有基因毒性的天然著色劑梔子黃(GY)。由于PSX/MSA具有多孔結(jié)構(gòu)且MSA分子中存在羧基,PSX/MSA對(duì)GY的吸附能力遠(yuǎn)高于單一的淀粉干凝膠(SX)和馬鈴薯淀粉。
[Abstract]:The chiral platform of potato starch (PSN) was constructed and applied to electrochemical recognition of tryptophan (Trp) enantiomers. Because PS has more affinity to D-Trp, PS is more inclined to combine with D-Trp through the interaction of host and guest. When L-Trp and D-Trp are in contact with the surface of PS modified electrode, L-Trp is more likely to be oxidized on the electrode surface through the left-handed double helix structure of PS. Therefore, PS modified electrode can be used to identify Trp enantiomers. The water contact angle experiment was used for the first time to prove the different affinity between PS and Trp enantiomers. At the same time, the PS-modified electrode is temperature-sensitive to the recognition of Trp enantiomers, which is due to the influence of temperature on the hydrogen bond formed between PS and the guest molecule TRP. It is worth noting that the efficiency of Trp enantiomeric recognition is obviously reduced by introducing hydroxypropyl groups into the host chain of PS, which may be due to the existence of additional steric hindrance in the process of host and guest reaction. CMC-CS composites can be formed by amidation of carboxyl groups on the chain and amino groups on the chain of chitosan. Compared with NaCMC and CS, when the mass ratio of NaCMC and CS is 1:1, the composite material (CMC-CSC: 1: 1) exhibits a three-dimensional porous network structure, because a large number of polar functional groups are exposed to CMC-CSC 1: 1) three-dimensional porous network structure. Thus, the hydrophilicity of CMC-CSN 1: 1) was improved significantly. In this chapter, the chiral interface of CMC-CS1: 1) composite was constructed and applied to electrochemical recognition of Trp enantiomers. Because of the high hydrophilic character of CMC-CSC (1: 1) and the good selectivity of Trp enantiomers to Trp enantiomers due to the different steric hindrance in the formation of hydrogen bonds with Trp enantiomers, the chiral interfaces have good selectivity to Trp enantiomers. At the same time, the mass ratio of NaCMC to CS and the preparation method were optimized. The results show that the chiral interface of CMC-CSN 1: 1) can accurately determine the content of D-Trp in racemate solution. During the gelation of potato starch, the thiomalic acid (MSA) molecule was introduced into the macromolecular chain of potato starch, thus destroying the inherent hydrogen bond between the potato starch chain and the chain. This process is verified by Fourier transform infrared (FTIR). After freeze-drying of potato starch modified by MSA, MSA modified porous starch xerogel (PSX / MSAA) was obtained. Finally, PSX/MSA was used to remove gene-toxic natural colorant Gardenia jasminoides. Because of the porous structure of PSX/MSA and the presence of carboxyl group in MSA, the adsorption ability of PSX / MSA to GY is much higher than that of single starch xerogel and potato starch.
【學(xué)位授予單位】：常州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB33

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本文編號(hào)：1957365