發(fā)布時(shí)間：2018-05-15 03:01

  本文選題：電化學(xué)發(fā)光免疫分析 + 免疫傳感器　； 參考：《蘇州大學(xué)》2015年碩士論文

【摘要】：電化學(xué)發(fā)光免疫分析是電化學(xué)發(fā)光(E C L)和免疫分析相結(jié)合的產(chǎn)物。作為一種新型的分析檢測(cè)手段,電化學(xué)發(fā)光免疫方法因具有高靈敏度、高特異性的特點(diǎn),已經(jīng)被廣泛地應(yīng)用于各種領(lǐng)域。本文制備了水溶性的量子點(diǎn)作為發(fā)光體以及石墨烯膠體金等新型的納米材料,在此基礎(chǔ)上建立了一種簡(jiǎn)單結(jié)構(gòu)的電化學(xué)發(fā)光免疫傳感器以及一種雙信號(hào)放大的電化學(xué)發(fā)光免疫傳感器,分別對(duì)食品中的受體激動(dòng)劑沙丁胺醇、苯乙醇胺A以及重金屬汞離子進(jìn)行了快速、靈敏的檢測(cè),對(duì)拓展基于量子點(diǎn)的電化學(xué)發(fā)光免疫方法的應(yīng)用有著重要的意義。本論文主要包括五個(gè)方面:1.介紹論文的研究背景及意義。闡述電化學(xué)發(fā)光免疫分析的基本理論及類型,著重歸納可以提高電化學(xué)發(fā)光免疫分析靈敏度的發(fā)光標(biāo)記物及固定化載體。2.制備了巰基乙酸(T G A)為穩(wěn)定劑的水溶性C d S e量子點(diǎn)、膠體金以及連有正電荷P D D A的還原型氧化石墨烯。借助紫外可見(jiàn)吸收(U V-v i s)光譜、高分辨率投射電鏡(T E M)、掃描電鏡(S E M)等表征手段對(duì)制備的材料進(jìn)行了表征。3.利用L-半胱胺和戊二醛,通過(guò)簡(jiǎn)單的A u-S鍵、-N H2與-C H O的特征官能團(tuán)之間的反應(yīng),以相對(duì)較短的鏈連接包被抗原和抗體,層層修飾,得到電化學(xué)發(fā)光免疫傳感器,并用于檢測(cè)沙丁胺醇。本實(shí)驗(yàn)最低檢出限為0.0 0 5 6 n g/m L,具有良好的穩(wěn)定性及特異性。4.利用本實(shí)驗(yàn)室制備的對(duì)二價(jià)汞離子具有高特異性的6-巰基煙酸的甲基汞包被抗原及單克隆抗體,通過(guò)簡(jiǎn)單結(jié)構(gòu)的電化學(xué)發(fā)光免疫傳感器對(duì)汞離子進(jìn)行了檢測(cè)。最低檢出限為0.0 0 6 2 n g/m L,明顯低于傳統(tǒng)的測(cè)定方法。對(duì)實(shí)際樣品的檢測(cè)進(jìn)一步證明該方法的可行性,同時(shí)為重金屬的檢測(cè)提供了新的思路。5.通過(guò)靜電作用創(chuàng)造性地制備了P-G R-Q D s-A u N P s-A b復(fù)合物探針,該探針增加了發(fā)光體量子點(diǎn)的量,提高了檢測(cè)苯乙醇胺A的靈敏度。借助紫外可見(jiàn)吸收(U V-v i s)光譜、高分辨率投射電鏡(T E M)、掃描電鏡(S E M)等表征手段對(duì)該復(fù)合物探針進(jìn)行了表征。
[Abstract]:Electrochemiluminescence immunoassay (ECL) is a combination of ECL and immunoassay. As a new analytical and detection method, electrochemiluminescence immunoassay has been widely used in various fields because of its high sensitivity and specificity. In this paper, water-soluble quantum dots (QDs) were prepared as luminescent materials and new nano-materials such as graphene colloidal gold. A simple electrochemical luminescent immunosensor and a double signal amplified electrochemical luminescent immunosensor were developed for the treatment of salbutamol, a receptor agonist in food, respectively. The rapid and sensitive detection of phenylethanolamine A and heavy metal mercury ions is of great significance for the development of electrochemiluminescence immunoassay based on quantum dots. This thesis mainly includes five aspects: 1. This paper introduces the research background and significance. The basic theory and types of electrochemiluminescence immunoassay (ECLIA) were described, and the luminescent markers and immobilized carrier. Water soluble C d S e quantum dots, colloidal gold and reduced graphene oxide with positively charged P D D A were prepared with thioglycolic acid (TGA) as stabilizer. The prepared materials were characterized by UV-vis absorption U V-v I S), high resolution projection electron microscopy (TEM), scanning electron microscopy (SEM) and so on. By using L- cysteamine and glutaraldehyde, electrochemiluminescence immunosensors were obtained by the reaction between simple characteristic functional groups of A-S bond, N-H _ 2 and -C H _ O, which were coated with antigens and antibodies with relatively short chains. It was used for the detection of salbutamol. The lowest detection limit of this experiment is 0.056 n / g / mL, which has good stability and specificity. 4. Mercury ion was detected by a simple electrochemical luminescent immunosensor using methylmercury coated antigen and monoclonal antibody of 6-mercaptonicotinic acid prepared in our laboratory. The lowest detection limit was 0.062 ng / mL, which was significantly lower than that of the traditional method. The feasibility of the method is further proved by the detection of actual samples, and a new way of thinking is provided for the detection of heavy metals. The P-G R-Q D s-A u N P s-A b complex probe was creatively prepared by electrostatic action. The probe increased the quantity of luminescent quantum dots and improved the sensitivity of detecting phenylethanolamine A. The composite probe was characterized by UV-vis absorption U V-v I S), high resolution projection electron microscopy (TEM), scanning electron microscopy (SEM) and so on.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;TP212

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