本文選題：聚乳酸納米粒子 + 免疫佐劑�。� 參考：《北京化工大學(xué)》2015年碩士論文

【摘要】：納米技術(shù)是一種很有發(fā)展?jié)摿Φ男滦图夹g(shù)。納米材料,以其粒徑小、比表面積大、性能優(yōu)越等特點,具有廣泛的應(yīng)用前景。生物可降解的高分子聚合物制備成的納米粒子在生物醫(yī)藥領(lǐng)域具有研究和應(yīng)用潛力。本論文主要研究聚乳酸納米粒子的制備方法并進行工藝優(yōu)化,將制備的聚乳酸納米粒子應(yīng)用于免疫佐劑的研究。取得的具體研究結(jié)果如下：1、研究納米沉淀法制備聚乳酸納米粒子,采用響應(yīng)面優(yōu)化法對制備工藝進行優(yōu)化,最優(yōu)條件制備得到的聚乳酸納米粒子粒徑為109.1 nm,PDI為0.085,粒徑均一,分散性良好；2、研究自乳化-溶劑揮發(fā)法制備聚乳酸納米粒子,對其進行工藝優(yōu)化。采用陰離子表面活性劑十二烷基硫酸鈉(SDS)制備表面帶負(fù)電的聚乳酸納米粒子,優(yōu)化工藝制備的聚乳酸納米粒子的粒徑為126.8 nm, PDI值為0.129, Zeta電位為-53.7 mV,粒徑均一,分散性良好；采用陽離子表面活性劑雙十二烷基二甲基溴化銨(DDAB),制備表面帶正電的聚乳酸納米粒子,優(yōu)化工藝制備得到納米粒子的粒徑為101.7 nm, PDI值為0.078,Zeta電位為+37 mV,粒徑均一,分散性良好；3、將帶正電的聚乳酸納米粒子作為免疫佐劑開展免疫效果研究,考察了100 nm、300 nm和1μm三種粒徑的聚乳酸顆粒,測定不同粒徑免疫佐劑效果,結(jié)果表明100 nm聚乳酸納米粒子能誘導(dǎo)更高水平的血清特異性抗體、細胞因子等表達,免疫效果更好；4、研究了表面帶負(fù)電的聚乳酸納米粒子的溶脹制孔,分別采用二次乳液溶脹法、載氣溶脹法和動態(tài)溶脹法三種方法進行納米粒子的制孔研究,對三種方法的實驗裝置、實驗條件進行優(yōu)化,成功地在納米粒子內(nèi)部制出孔道。
[Abstract]:Nanotechnology is a new technology with great potential for development. Nanomaterials, with their small particle size, large specific surface area, superior performance and other characteristics, have a wide range of application prospects. The nanoparticles prepared by biodegradable polymer have the potential of research and application in the field of biomedicine. In this paper, the preparation method of polylactic acid nanoparticles was studied and the preparation process was optimized. The prepared polylactic acid nanoparticles were applied to the study of immune adjuvant. The specific results obtained are as follows: 1. The preparation of polylactic acid nanoparticles by nano-precipitation method was studied. The preparation process was optimized by response surface optimization method. The particle size of polylactic acid nanoparticles prepared under the optimum conditions was 0.085 and 0.085, and the particle size was uniform. Poly (lactic acid) nanoparticles were prepared by self-emulsifying-solvent volatilization method. Polylactic acid nanoparticles with negative charge were prepared by anionic surfactant sodium dodecyl sulfate. The particle size, PDI value and Zeta potential were 126.8 nm, 0.129 and -53.7 MV, respectively. The polylactic acid nanoparticles with positive charge on the surface were prepared by using the cationic surfactant didodecyl dimethylammonium bromide (DDABN). The particle size of the nanoparticles was 101.7 nm, the PDI value was 0.078% Zeta potential was 37 MV, and the particle size was uniform. The immunological effect of polylactic acid nanoparticles with positive charge as immune adjuvant was studied. Three kinds of polylactic acid particles (100nm ~ 300nm and 1 渭 m) were investigated. The results showed that 100nm polylactic acid nanoparticles could induce a higher level of serum specific antibodies, cytokines and other expressions, and the immune effect was better. The swelling of polylactic acid nanoparticles with negative charge on the surface was studied. The pore preparation of nanoparticles was studied by means of secondary emulsion swelling method, gas carrying swelling method and dynamic swelling method respectively. The experimental equipment and experimental conditions of the three methods were optimized, and the pore channels were successfully made in the nanoparticles.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：O633.14;TB383.1

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