本文選題：金納米粒子 + 信號(hào)傳導(dǎo)��； 參考：《西南大學(xué)》2015年碩士論文

【摘要】：金納米粒子制備簡(jiǎn)單,粒徑均一可控,化學(xué)性質(zhì)穩(wěn)定,易于表面功能化,并具有強(qiáng)烈的局域表面等離子體共振(Localized surface plasmon resonance, LSPR)等光學(xué)性質(zhì),成為目前研究最多的納米材料之一,已廣泛應(yīng)用于催化、生物醫(yī)藥、分析檢測(cè)等領(lǐng)域。本碩士學(xué)位論文利用金納米粒子獨(dú)特的光學(xué)性能,通過(guò)合適的生物偶聯(lián)和／或功能化,將其應(yīng)用于免疫分析、重金屬離子檢測(cè)等生化分析中的信號(hào)傳導(dǎo)和放大,具體包括以下三部分工作：(1)采用金納米粒子增強(qiáng)表面等離子體共振成像(surface plasmon resonance imaging, SPRi)的信號(hào),用于靈敏免疫檢測(cè)小分子真菌毒素。各種真菌毒素的檢測(cè)對(duì)于食品安全具有重要意義。SPRi是一種可同時(shí)進(jìn)行多目標(biāo)物免疫檢測(cè)分析的光學(xué)成像技術(shù),廣泛應(yīng)用于生物大分子免疫檢測(cè)和相互作用研究。然而,在免疫法檢測(cè)小分子真菌毒素時(shí),SPRi的靈敏度有限,不能滿足檢測(cè)的實(shí)際需要。本文采用競(jìng)爭(zhēng)免疫模式,利用金納米粒子放大SPRi信號(hào),對(duì)多種小分子毒素進(jìn)行了同時(shí)檢測(cè),實(shí)現(xiàn)了高選擇性、高靈敏度同時(shí)檢測(cè)黃曲霉毒素B1(Aflatoxin B1, AFBl)、赭曲霉素(Ochratoxin A, OTA)和玉米烯酮(Zearalenone, ZEN)三種典型的真菌毒素,其檢測(cè)限分別為8、30和15 pg mL-’,動(dòng)態(tài)范圍達(dá)到3個(gè)數(shù)量級(jí)。(2)采用金納米粒子及其表面原位引發(fā)聚合進(jìn)行SPRi的連續(xù)信號(hào)放大,實(shí)現(xiàn)在大濃度范圍靈敏免疫檢測(cè)腫瘤標(biāo)志物�，F(xiàn)有的SPRi信號(hào)放大方法存在定量關(guān)系差、難以在大濃度范圍內(nèi)對(duì)目標(biāo)物進(jìn)行定量檢測(cè)。本部分工作采用SPRi在夾心免疫法模式下對(duì)腫瘤標(biāo)志物進(jìn)行免疫檢測(cè),通過(guò)引入同時(shí)負(fù)載抗體蛋白和表面引發(fā)原子轉(zhuǎn)移自由基聚合(Surface initiated-atom transfer radical polymerization, SI-ATRP)表面引發(fā)劑的金納米粒子實(shí)現(xiàn)SPRi信號(hào)的第一次放大；此后,在芯片表面以金納米粒子上的引發(fā)劑為活性位點(diǎn)原位引發(fā)ATRP,實(shí)現(xiàn)第二次SPRi信號(hào)放大。采用該方法對(duì)典型的腫瘤標(biāo)志物甲胎蛋白(a-fetoprotein, AF P)在10%的人血清中進(jìn)行檢測(cè),檢測(cè)限達(dá)到1.0ng mL-1,檢測(cè)的動(dòng)態(tài)范圍達(dá)到2個(gè)數(shù)量級(jí)。(3)基于2,2'-聯(lián)吡啶誘導(dǎo)金納米粒子聚集的可視化汞離子(Hg2+)檢測(cè)。重金屬離子Hg2+是高毒性污染物,簡(jiǎn)單可靠的現(xiàn)場(chǎng)檢測(cè)方法非常重要。本部分工作發(fā)展了一種基于2,2'-聯(lián)吡啶誘導(dǎo)金納米粒子聚集的可視化Hg2+檢測(cè)方法：2,2'-聯(lián)吡啶(2,2'-Bipyridyl, Bipy)能誘導(dǎo)金納米粒子發(fā)生可控的線性聚集,伴隨溶液由紅到藍(lán)的顏色變化；而Hg2+能在金納米粒子表面形成Au-Hg合金層,從而有效抑制Bipy引起的金納米粒子聚集。基于此規(guī)律建立的可視化Hg2+檢測(cè)方法選擇性良好,檢測(cè)動(dòng)態(tài)范圍為0.2-2μM,采用簡(jiǎn)單的可見光分光光度計(jì),其檢測(cè)限可進(jìn)一步降低到38 nM。同時(shí),該方法對(duì)樣品pH值和離子強(qiáng)度不敏感,對(duì)實(shí)際樣品表現(xiàn)出良好的檢測(cè)可靠性和選擇性,具有快速現(xiàn)場(chǎng)檢測(cè)Hg2+的潛力。
[Abstract]:Gold nanoparticles have many advantages such as simple preparation, uniform and controllable particle size, stable chemical properties, easy surface functionalization, and strong optical properties such as localized surface plasmon resonance (Lspa), so they have become one of the most studied nanomaterials.Has been widely used in catalysis, biomedicine, analytical detection and other fields.Using the unique optical properties of gold nanoparticles, this master thesis applies the gold nanoparticles to signal transduction and amplification in biochemical analysis, such as immunoassay, heavy metal ion detection and so on, through appropriate biological coupling and / or functionalization.It includes the following three parts: 1) the use of gold nanoparticles enhanced surface plasmon resonance imaging (SPR) signal for sensitive immunoassay of small molecular mycotoxins.Detection of various mycotoxins is of great significance for food safety. SPRi is an optical imaging technique which can be used for simultaneous detection and analysis of multi-object immunoassay. It is widely used in biomolecular immunoassay and interaction research.However, the sensitivity of SPRi in detection of small molecular mycotoxins by immunoassay is limited and can not meet the practical needs of detection.In this paper, a competitive immune model was used to amplify the SPRi signal by gold nanoparticles, and a variety of small molecular toxins were simultaneously detected, which achieved high selectivity.The dynamic range is up to 3 orders of magnitude.) Gold nanoparticles and in situ polymerization of gold nanoparticles are used to amplify the continuous signal of SPRi and to detect tumor markers in a wide range of sensitive immunoassay.The existing SPRi signal amplification methods have poor quantitative relationship, so it is difficult to detect the target in a wide range of concentrations.In this part, SPRi was used to detect tumor markers in sandwich immunoassay.The first amplification of SPRi signal was achieved by the introduction of gold nanoparticles loaded with both antibody protein and surface-initiated atom transfer radical polymerization surface initiated-atom transfer radical polymerization (SI-ATRP) surface initiator.On the surface of the chip, the initiator on the gold nanoparticles was used as the active site to initiate the ATRPs in situ to amplify the second SPRi signal.This method was used to detect the typical tumor marker Afetoprotein (AFPin) in 10% of human serum.The detection limit is up to 1.0ng mL-1, and the dynamic range of detection is two orders of magnitude. The detection is based on the visible mercury ion Hg2 based on 2zapyridine-bipyridine induced gold nanoparticles aggregation.Heavy metal ion (Hg2) is a highly toxic pollutant, and a simple and reliable field detection method is very important.In this part of our work, we developed a visual Hg2 method for the detection of gold nanoparticles based on the aggregation of gold nanoparticles induced by 2zapyridine and bipyridine, which can induce the controlled linear aggregation of gold nanoparticles with the color change of solution from red to blue.Hg2 can form Au-Hg alloy layer on the surface of gold nanoparticles, which can effectively inhibit the aggregation of gold nanoparticles induced by Bipy.The visual Hg2 detection method based on this rule has good selectivity, and the detection dynamic range is 0.2-2 渭 M. the detection limit can be further reduced to 38 nm by using a simple visible light spectrophotometer.At the same time, the method is not sensitive to the pH value and ionic strength of the sample, and has good reliability and selectivity for the actual sample detection. It has the potential of rapid field detection of Hg2.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O614.123;TB383.1;O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Utilization of unmodified gold nanoparticles in colorimetric detection[J];Science China(Physics,Mechanics & Astronomy);2011年10期

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本文編號(hào)：1740246