發(fā)布時(shí)間：2018-04-03 20:06

  本文選題：核殼結(jié)構(gòu)　切入點(diǎn)：層層自組裝　出處：《天津大學(xué)》2015年碩士論文

【摘要】：目前,由于具備在不同環(huán)境刺激下對(duì)抗癌藥物,標(biāo)記染料的控制釋放,以及其本身固有的生物相容性,基于多糖類材料制備的多功能納米粒備受研究者青睞。本文主要利用殼聚糖(Cs)和聚乙烯醇磷酸酯(PPVA)作為靜電多層材料,借助層層自組裝技術(shù),交替沉積在3-氨丙基三乙氧基硅烷改性修飾的二氧化硅球表面制備了多功能納米粒。通過透射電子顯微鏡分析和粒徑分布表征,證明了納米粒具備良好的球形形貌。7-羥基香豆素和羅丹明B作為二種模擬藥物分別裝載在了核層和殼層之中。通過激光共聚焦顯微鏡證明了核殼結(jié)構(gòu),進(jìn)一步驗(yàn)證了藥物的分布位置。通過研究不同pH環(huán)境下,7-羥基香豆素和羅丹明B的共釋放行為,表明了SiO2(PPVA/Cs)n這種多功能核殼納米粒在藥物遞送領(lǐng)域具有嶄新的應(yīng)用前景。另一方面,我們還利用預(yù)凝膠方法制備了多糖基(果膠,卡拉膠)羥基磷灰石的核殼納米粒。通過固定多糖結(jié)構(gòu)單元與鈣離子摩爾比,調(diào)節(jié)羥基磷灰石的鈣磷比,研究了多糖分子與羥基磷灰石的相互作用,通過透射電鏡觀察了納米粒的形貌特征,最終確定了以羥基磷灰石晶體為核,陰離子多糖為殼的無機(jī)-有機(jī)雜化核-殼納米粒的制備方法。通過在納米粒表面進(jìn)行靜電層層自組裝得到了核殼納米雜化材料,材料不僅顯示出了果膠和卡拉膠的生物相容性,而且具備羥基磷灰石的pH可降解性。最后,通過研究?jī)煞N納米粒在不同pH下對(duì)羅丹明6G的釋放行為,得出結(jié)論：在酸性條件下由于羥基磷灰石的溶解導(dǎo)致水溶性藥物羅丹明6G從納米粒中大量釋放。研究結(jié)果表明,基于生物多糖形成的納米核殼材料在生物醫(yī)藥領(lǐng)域具有潛在的研究?jī)r(jià)值。
[Abstract]:At present, due to the ability in different environment stimulation on anticancer drug controlled release of dye, and its inherent biocompatibility, multifunctional nanoparticles polysaccharide material preparation has attracted great attention from researchers. This paper mainly based on the use of chitosan and polyvinyl alcohol (Cs) phosphate (PPVA) as an electrostatic multilayer material with layers the self-assembly technology, multifunctional nanoparticles prepared by alternating silica spheres surface were deposited on 3- aminopropyltriethoxysilane modified. By transmission electron microscopy analysis and characterization of particle size distribution, proved that the nanoparticles have good spherical morphology of.7- hydroxycoumarin and Rhodamine B as two kinds of drugs were loaded in the nuclear simulation layer and shell. Using laser scanning confocal microscopy proved that the core-shell structure, further verify the location of drugs. Through the study of different pH environment, 7- hydroxy aromatic Legumin and CO release behavior of rhodamine B, showed that SiO2 (PPVA/Cs) n this multifunctional core shell nanoparticles in drug delivery field has new application prospects. On the other hand, we also use the pre gel method based on polysaccharide preparation (pectin, carrageenan) core-shell nanoparticles of hydroxyapatite. By fixing the polysaccharide the structure unit and the calcium ion molar ratio of calcium and phosphorus regulated hydroxyapatite ratio, interaction of polysaccharide molecules and hydroxyapatite, the morphology of nanoparticles was observed by transmission electron microscopy, and finally determines the hydroxyapatite crystal nucleus, anionic polysaccharide to a preparation method of shell organic-inorganic hybrid core-shell nanoparticles by. Electrostatic self-assembly in the surface of the nanoparticles obtained core-shell nano hybrid materials, materials not only shows the compatibility of pectin and carrageenan organisms, but with hydroxyapatite pH Degradation. Finally, through the study of two kinds of nanoparticles in different pH on Luo Danming 6G's release behavior, draw a conclusion: under acidic conditions due to hydroxyapatite dissolution causes the water soluble drug from Luo Danming 6G to release large amounts of nanoparticles. The results show that the nano core-shell materials biological polysaccharide formation have a potential value in based on the field of biology and medicine.

【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;TQ460.4

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本文編號(hào)：1706714