本文選題：水熱制備法　切入點：生物量子點　出處：《南昌大學》2015年碩士論文

【摘要】：生物量子點(bio-dots)和熒光碳量子點(CQDs)是最近興起的新穎熒光納米材料,相對于傳統(tǒng)的半導體量子點而言,具有非常明顯的優(yōu)勢。除了光學性能優(yōu)良,bio-dots和CQDs還具有的毒性低、環(huán)境友好、制備方法簡單、成本低等優(yōu)點,讓它們在化學和生物傳感領域有很大的應用前景。本論文利用水熱方法成功制備了bio-dots和CQDs,通過對熒光量子點的表征,概述了其基本特征并深入探討了其在分析化學領域的應用。研究的主要內容如下:(1)以富含C堿基的DNA鏈作原料,通過低溫水熱法制備了熒光bio-dots。采用透射電鏡(TEM)、原子力顯微鏡(AFM)對bio-dots形貌進行了表征,結果表明,其粒徑約為16 nm,厚度約為0.8 nm。用X-射線光電子能譜(XPS)對bio-dots成分進行了分析,結果表明,bio-dots既保留了DNA的C堿基又與DNA的成分大致相同。利用熒光光譜(FL)對bio-dots進行了光譜表征,其熒光發(fā)射波長不隨激發(fā)波長的變化而改變,當激發(fā)波長從260 nm增加320 nm時,bio-dots的發(fā)射波長均為420 nm。此外,實驗結果表明bio-dots的耐光漂白性強,然而pH對bio-dots的發(fā)光有較大影響。(2)基于bio-dots與Ag+的相互作用構建光致發(fā)光傳感平臺,用于谷胱甘肽(GSH)和谷胱甘肽還原酶(GR)活性的檢測。利用bio-dots與DNA結構類似的特性,以Ag+作為橋梁與bio-dots的C堿基配位形成C-Ag+-C配合物,從而破壞bio-dots的發(fā)光中心,導致bio-dots熒光猝滅。由于Ag+與巰基的配位能力比與C堿基更強,當加入GSH時,Ag+首先與GSH結合,使bio-dots結構不被破壞,因而熒光不猝滅,據(jù)此實現(xiàn)GSH定量分析。此外,基于GR-催化酶促反應對GR活性和氧化型谷胱甘肽(GSSH)進行了定量分析并用于抑制劑的篩選。(3)以尿酸作為碳源,180oC水熱反應8 h即可獲得藍色熒光的CQDs。銅在許多生物進程中被認為是一種重要的微量元素,廣泛的參與生命的進程,人體內銅離子超標會破壞人體中的代謝平衡產(chǎn)生一系列的疾病,如門克斯病、威爾遜病、阿爾茨海默病和普里昂疾病等。本文利用熒光CQDs表面豐富的氮、氧基團與Cu2+發(fā)生配位作用,導致熒光猝滅,根據(jù)熒光猝滅程度對Cu2+進行了定量分析,檢測Cu2+線性范圍為1-15μM。
[Abstract]:Biological quantum dots (bio-dotss) and fluorescent carbon quantum dots (CQDs) are novel fluorescent nanomaterials, which have obvious advantages over traditional semiconductor quantum dots. In addition to their excellent optical properties, bio-dots and CQDs have low toxicity. Due to their advantages of environmental friendliness, simple preparation method and low cost, they have great application prospects in chemical and biological sensing fields. In this paper, bio-dots and CQDswere successfully prepared by hydrothermal method, and characterized by fluorescence quantum dots. The main contents of this study are as follows: (1) the DNA chain rich in C bases is used as raw material. Fluorescence bio-dots were prepared by hydrothermal method at low temperature. The morphology of bio-dots was characterized by TEM and AFM. The results showed that the particle size was about 16 nm and the thickness was about 0.8 nm. The composition of bio-dots was analyzed by X-ray photoelectron spectroscopy (XPS). The results show that the C base of DNA is retained and the composition of bio-dots is about the same as that of DNA. The spectra of bio-dots are characterized by fluorescence spectra. The fluorescence emission wavelength does not change with the change of excitation wavelength. When the excitation wavelength is increased from 260 nm to 320 nm, the emission wavelengths of bio-dots are 420nm. In addition, the experimental results show that the photobleaching resistance of bio-dots is strong, but pH has a great effect on the luminescence of bio-dots. It is used for the detection of glutathione glutathione (GSH) and glutathione reductase (Glutathione reductase) activity. Using the similar structure of bio-dots and DNA, Ag is used as a bridge to coordinate with C base of bio-dots to form C-Ag C complex, which destroys the luminescent center of bio-dots. The fluorescence quenching of bio-dots is caused. Because Ag has stronger coordination ability with sulfhydryl than with C base, when GSH is added, Ag binds to GSH first, so the structure of bio-dots is not destroyed, thus the fluorescence is not quenched, so the quantitative analysis of GSH is realized. Gr activity and oxidized glutathione (GSSH) were quantitatively analyzed based on GR-catalyzed enzymatic reaction and were used to screen inhibitors. The blue fluorescence CQDs. copper was obtained by hydrothermal reaction of 180oC with uric acid as carbon source for 8 h. Cheng Zhong is considered to be an important trace element. With extensive participation in the process of life, excessive copper ions in the human body can disrupt the metabolic balance in the body and produce a range of diseases, such as Menkes disease, Wilson's disease, In this paper, the rich nitrogen and oxygen groups on the surface of fluorescent CQDs were used to coordinate with Cu2, which led to fluorescence quenching. The quantitative analysis of Cu2 was carried out according to the degree of fluorescence quenching. The linear range of detection of Cu2 was 1-15 渭 M.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1

【共引文獻】

相關期刊論文 前3條

1 王莉;呂婷;阮楓萍;鄧德剛;徐時清;;水熱法制備的熒光碳量子點[J];發(fā)光學報;2014年06期

2 袁鳴;鐘睿博;張景偉;李潔;張盼;馬宇星;張萍;韓小寧;張峰;;熒光碳點在生物醫(yī)學領域中的應用研究進展[J];基因組學與應用生物學;2015年01期

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相關碩士學位論文 前3條

1 雒連春;熒光碳納米粒子的微波加熱法合成、表征及在細胞成像中的應用[D];陜西師范大學;2013年

2 趙永強;基于單一基碳點白光熒光粉的LED[D];太原理工大學;2015年

3 何玉珩;白光LED用單一基質碳點基熒光粉的制備與表征[D];太原理工大學;2015年

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本文編號：1654870