本文選題：熒光探針　切入點(diǎn)：溫敏高聚物　出處：《華東師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：納米材料具有顆粒小、可修飾性強(qiáng)、特異性識(shí)別等特性,因此其復(fù)合材料在化學(xué)分析、生物傳感和醫(yī)療等領(lǐng)域扮演著重要角色。在醫(yī)學(xué)上,納米材料的應(yīng)用常側(cè)重于生物傳感和藥物運(yùn)輸。癌癥的早期診斷與有效治療仍是當(dāng)前醫(yī)療上的一大瓶頸,本文設(shè)計(jì)合成了一種可用于各種比率化的溫度相關(guān)檢測(cè)以及癌癥診療應(yīng)用的溫敏核殼復(fù)合熒光納米材料(CD-MSN@UP38),并將其應(yīng)用在細(xì)胞內(nèi)溫度檢測(cè)和活體癌癥診療中。該材料作為溫度傳感器時(shí),裝載極性敏感染料BBD并結(jié)合熒光壽命顯微成像技術(shù),能做到細(xì)胞內(nèi)溫度的比率化準(zhǔn)確檢測(cè)以及單個(gè)細(xì)胞內(nèi)溫度分布,具有極高的時(shí)空分辨率和溫度分辨(0.2℃)。更有意思的是,CD-MSN@UP38粒子可以進(jìn)一步作為溫控藥物傳遞系統(tǒng)應(yīng)用于癌癥治療,由癌細(xì)胞本身的熱效應(yīng)觸發(fā)釋藥。該智能載藥物治療系統(tǒng)在活體實(shí)驗(yàn)中顯示了高效療和低副作用。另一方面,在生物分析領(lǐng)域中,由于人臍帶間充質(zhì)干細(xì)胞(hMSCs)可以分化為神經(jīng)干細(xì)胞(NSCs),在中樞神經(jīng)系統(tǒng)紊亂和細(xì)胞置換療法中有巨大的潛在應(yīng)用。端粒酶(Telomerase,TE)可以添加端粒(TTAGGG)n重復(fù)到人的染色體末端,與干細(xì)胞壽命息息相關(guān)。然而,其活性在維持hMSCs細(xì)胞增殖過程中的生物學(xué)特性以及分化潛力變化中扮演的角色仍是未知數(shù)。本文開拓了一種新型誘導(dǎo)細(xì)胞自發(fā)合成熒光cDNA銀納米簇(cDNA-AgNCs),長(zhǎng)期實(shí)時(shí)成像hMSCs細(xì)胞增殖和分化過程中TE活性的變化。該生物原位合成的以DNA為模板的熒光AgNCs尺寸小、光穩(wěn)定性好且生物相容性優(yōu)異,突破了外源合成的納米復(fù)合材料易被免疫排斥而不能長(zhǎng)期在生物內(nèi)傳感的瓶頸。
[Abstract]:Nanomaterials play an important role in the fields of chemical analysis, biosensor and medical treatment because of their small particle size, strong modifiability, specificity recognition and so on. The application of nanomaterials is often focused on biosensors and drug transport. Early diagnosis and effective treatment of cancer remains a major medical bottleneck. In this paper, we have designed and synthesized a thermo-sensitive core-shell composite fluorescent nanomaterial, CD-MSN @ UP38, which can be used for various ratios of temperature correlation detection and cancer diagnosis and treatment. The material has been applied to intracellular temperature detection and in vivo cancer diagnosis and treatment. When the material acts as a temperature sensor, Loaded with polarity sensitive dye BBD and fluorescence lifetime microimaging technology, the ratio of intracellular temperature and the temperature distribution in a single cell can be measured accurately. It has a very high space-time resolution and a temperature resolution of 0.2 鈩，

本文編號(hào)：1589548