本文關(guān)鍵詞： 納米光電材料 透射電子顯微鏡 SERS 大腸桿菌　出處：《青島大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本論文主要對零維金屬納米材料(鍺納米晶和銀納米顆粒)的制備、表征及應(yīng)用進行了研究。利用透射電子顯微學(xué)技術(shù)對鍺納米晶的微觀結(jié)構(gòu)進行研究,為特定尺寸鍺納米晶的制備提供理論基礎(chǔ);以銀納米顆粒為活性基底,利用表面增強拉曼散射(SERS)技術(shù)對單細胞進行檢測,成功區(qū)分兩種不同細胞壁的酵母細胞,對細菌分泌物進行檢測,為耐藥菌的快速檢測提供數(shù)據(jù)支撐。本論文研究內(nèi)容主要分為以下四章:第一章首先對納米材料在新能源以及生物醫(yī)學(xué)領(lǐng)域的應(yīng)用進行綜述,其次對鍺納米晶和SERS理論進行簡介。第二章通過離子注入法在非晶二氧化硅薄膜中制備鍺納米晶,并對其尺寸分布及微觀結(jié)構(gòu)進行研究。結(jié)果表明,鍺納米晶的尺寸和粒徑差異隨注入劑量的增加而不斷增加;最大的鍺納米晶在鍺濃度最高的地方形成;定義了1~10 nm鍺納米晶尺寸的閾值,并估算了參與生成鍺納米晶的鍺含量大約在40%~60%。第三章通過化學(xué)還原法制備銀納米顆粒并以此為SERS活性基底,在單細胞水平進行研究。結(jié)果表明,含有表面保護劑聚乙烯吡咯烷酮(PVP)的銀納米顆粒重現(xiàn)性較好;SERS可以很好的區(qū)分兩種細胞壁不同的酵母細胞。第四章確定了大腸桿菌應(yīng)激反應(yīng)SERS信號來自于細菌分泌物;發(fā)現(xiàn)變異鏈球菌與大腸桿菌應(yīng)激反應(yīng)不同,可以用于細菌鑒別;基于應(yīng)激反應(yīng)現(xiàn)象對大腸桿菌耐藥性進行探究,為開發(fā)快速檢測耐藥菌提供數(shù)據(jù)支持。
[Abstract]:In this paper, the preparation, characterization and application of zero-dimensional metallic nanomaterials (germanium nanocrystals and silver nanoparticles) were studied. The microstructure of germanium nanocrystals was studied by transmission electron microscopy (TEM). To provide a theoretical basis for the preparation of germanium nanocrystals of specific size, silver nanoparticles were used as active substrates, surface enhanced Raman scattering (SERS) technique was used to detect single cells, and two kinds of yeast cells with different cell walls were successfully distinguished. The detection of bacterial secretions provides data support for rapid detection of drug-resistant bacteria. This paper is mainly divided into four chapters: the first chapter summarizes the application of nanomaterials in the field of new energy and biomedicine. In chapter 2, germanium nanocrystals were prepared in amorphous silica thin films by ion implantation, and the size distribution and microstructure of germanium nanocrystals were studied. The size and particle size difference of germanium nanocrystals increase with the increase of implantation dose; the largest germanium nanocrystals are formed at the highest germanium concentration; the threshold value of 1ng-10nm germanium nanocrystalline size is defined. The content of germanium involved in the formation of germanium nanocrystals was estimated to be about 400.In chapter 3, silver nanoparticles were prepared by chemical reduction method and used as SERS active substrates. Silver nanoparticles containing polyvinylpyrrolidone (PVP) have good reproducibility and can distinguish two different yeast cells with different cell walls. Chapter 4th confirmed that the SERS signal of Escherichia coli stress reaction came from bacterial secretion. It was found that the stress reaction of Streptococcus mutans was different from that of Escherichia coli, which could be used to identify bacteria, and to explore the drug resistance of Escherichia coli based on the phenomenon of stress reaction, to provide data support for rapid detection of drug-resistant bacteria.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB383.1

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