本文關(guān)鍵詞： 鉑納米簇 復(fù)合納米簇 腫瘤成像 腫瘤光熱治療　出處：《東南大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：惡性腫瘤嚴(yán)重威脅人類健康,是人類死亡的最大殺手之一,早期診斷和有效治療是減少腫瘤死亡率的最有效途徑。近年來,納米技術(shù)的不斷發(fā)展及大量新型納米材料的出現(xiàn)為腫瘤的診治開辟了新的思路。在腫瘤的診斷方面,以熒光納米材料為探針的細(xì)胞活體成像技術(shù)得到廣泛的應(yīng)用,特別是發(fā)展無毒、熒光強(qiáng)度優(yōu)良、靶向性好的納米材料顯得更加有意義；在腫瘤的治療方面,利用納米材料作為藥物輸送載體,利用納米材料作光敏劑進(jìn)行光動(dòng)力和光熱輔助治療等都取得很好的效果。鉑納米材料除了用作傳統(tǒng)意義上的催化劑外,近年來也有一些研究關(guān)于在腫瘤的成像檢測(cè)和治療方面的應(yīng)用,本論文主要探討和研究了鉑納米簇的合成新方法及其在生物成像、抗腫瘤、光催化等方面的應(yīng)用。木論文的工作分為如下三個(gè)部分：1.鉑納米簇的化學(xué)合成新方法及其在腫瘤的成像和光熱治療中的應(yīng)用研究我們利用還原性谷胱甘肽、抗壞血酸(維生素C)的協(xié)同和保護(hù)作用一步法快速制備了熒光鉑納米簇,方法簡(jiǎn)單綠色環(huán)保,經(jīng)過表征發(fā)現(xiàn)這種鉑納米簇的生物相容性非常好、熒光穩(wěn)定、靶向性強(qiáng),能用于腫瘤細(xì)胞的特異性識(shí)別,并且發(fā)現(xiàn)在紅外光照下能有效地殺死腫瘤細(xì)胞,這表明它在腫瘤的早期診斷和光熱治療中有著潛在的應(yīng)用前景。2.鉑納米簇的原位生物合成方法及其在腫瘤的精確成像和光動(dòng)力學(xué)治療中的應(yīng)用研究我們發(fā)現(xiàn)腫瘤細(xì)胞能夠特異性地誘導(dǎo)H2PtCl6還原并且原位生物合成熒光鉑納米簇,而在正常細(xì)胞中不能合成相關(guān)熒光納米簇,以此發(fā)現(xiàn)了一種全新的腫瘤成像新方法。同時(shí),在加入卟啉衍生物后進(jìn)行紅外光照,發(fā)現(xiàn)能夠有效地協(xié)同抑制腫瘤的增長(zhǎng),在腫瘤的光動(dòng)力學(xué)治療中具有潛在的應(yīng)用前景。3.鉑金銀復(fù)合納米簇材料的合成新方法及其催化性能的研究我們利用還原性谷胱甘肽、抗壞血酸的協(xié)同和保護(hù)作用快速制備了鉑金納米簇和鉑金銀復(fù)合納米簇。經(jīng)過表征發(fā)現(xiàn)這種復(fù)合納米材料能夠高效地催化對(duì)硝基苯酚還原為對(duì)氨基苯酚,同時(shí)由于其優(yōu)良的熒光性能,無毒的生理特性,可潛在地被用于生物成像探針。
[Abstract]:Malignant tumor is a serious threat to human health and is one of the biggest killers of human mortality. Early diagnosis and effective treatment is the most effective way to reduce tumor mortality in recent years. The continuous development of nanotechnology and the emergence of a large number of new nano-materials have opened up new ideas for the diagnosis and treatment of tumor. In vivo imaging technology with fluorescent nanomaterials as probes has been widely used, especially in the development of non-toxic, fluorescence intensity, good targeting of nanomaterials is more meaningful; In the treatment of cancer, nanomaterials are used as the carrier of drug delivery. The use of nano-materials as Guang Min for photodynamic and photothermal auxiliary therapy has achieved good results. Platinum nanomaterials are used as catalysts in the traditional sense. In recent years, there have been some studies on the application of tumor imaging detection and treatment. In this paper, we mainly discuss the new synthesis method of platinum nanoclusters and its application in biological imaging and anti-tumor. Photocatalysis and other applications. The work of this paper is divided into three parts as follows: 1. The new method of chemical synthesis of platinum nanoclusters and its application in tumor imaging and photothermal therapy We use reduced glutathione. The coordination and protection of ascorbic acid (vitamin C) produced the fluorescent platinum nanoclusters quickly by one-step method. The method was simple green and environmentally friendly. It was found that the platinum nanoclusters had very good biocompatibility and stable fluorescence. It can be used for the specific recognition of tumor cells and can kill tumor cells effectively under infrared light. 2. In situ biosynthesis of platinum nanoclusters and its application in accurate imaging and photodynamic therapy of tumors. Tumor cells can specifically induce H2PtCl6 reduction and in situ biosynthesis of fluorescent platinum nanoclusters. However, we can not synthesize the related fluorescent nanoclusters in normal cells, so we can find a new method of tumor imaging. At the same time, we add porphyrin derivatives to infrared radiation. It has been found that it can effectively inhibit the growth of tumor. There are potential applications in photodynamic therapy of tumors .3.The synthesis of platinum and silver nanoclusters and their catalytic properties are studied. We use reductive glutathione. Platinum nanoclusters and platinum / silver nanoclusters were rapidly prepared by the synergistic and protective effects of ascorbic acid. It was found that the composite nano-materials could efficiently catalyze the reduction of p-nitrophenol to p-aminophenol. At the same time, because of its excellent fluorescence properties and nontoxic physiological properties, it can potentially be used as a biological imaging probe.
【學(xué)位授予單位】：東南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R318;O614.826;TB383.1

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