本文關(guān)鍵詞：DNA-金納米粒子直接復(fù)合體系穩(wěn)定性與可逆聚集行為研究　出處：《河北大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：金納米粒子(AuNPs)由于具有獨特的光、電、磁等性質(zhì),在環(huán)境、藥物、食品等領(lǐng)域都有廣泛的應(yīng)用。最簡單常用的制備AuNPs的方法是檸檬酸鈉還原金的鹽溶液。然而,該法制備的AuNPs在酸性條件或高離子強(qiáng)度環(huán)境中由于金納米粒子表面電荷的改變易發(fā)生聚集。在復(fù)雜環(huán)境下保持穩(wěn)定是AuNPs廣泛應(yīng)用的前提。獲得穩(wěn)定AuNPs的有效方法之一就是在制備時加入一些修飾劑對其表面進(jìn)行修飾,然而這些修飾方法仍然存在一些缺點。小牛胸腺DNA具有良好的生物相容性、可降解和無毒性等特點,已經(jīng)廣泛應(yīng)用于生物和醫(yī)學(xué)領(lǐng)域。本文以小牛胸腺DNA為金納米的穩(wěn)定劑,采用直接復(fù)合的方法制備DNA-AuNPs直接復(fù)合體系,并研究了該復(fù)合體系在不同pH值以及不同金屬離子強(qiáng)度的緩沖溶液中的穩(wěn)定性,根據(jù)溶液顏色變化以及紫外吸收峰的改變,初步探討了外源性因素對DNA-AuNPs直接復(fù)合體系穩(wěn)定性的影響與可逆聚集行為。本論文的主要研究工作如下:pH值對DNA-AuNPs直接復(fù)合體系穩(wěn)定性的影響與可逆聚集行為研究。應(yīng)用紫外可見吸收光譜(UV-Vis)、原子力顯微鏡(AFM)、透射電子顯微鏡(TEM)等測試方法對AuNPs溶液進(jìn)行表征。結(jié)果表明,DNA-AuNPs直接復(fù)合體系在pH為2-11的緩沖溶液中均可穩(wěn)定存在,當(dāng)溶液中有大量H+存在時,DNA-AuNPs直接復(fù)合體系發(fā)生聚集,加入OH-后未發(fā)生解聚集行為。在冷凍解凍循環(huán)方面,DNA修飾的AuNPs穩(wěn)定性比AuNPs原溶液強(qiáng)。金屬離子對DNA-AuNPs直接復(fù)合體系穩(wěn)定性的影響與可逆聚集行為研究。結(jié)果表明,DNA-AuNPs在一定量(0-300μL)低價態(tài)金屬離子(Na+、K+、Mg~(2+)、Ni~(2+))存在下具有良好的穩(wěn)定性,但是加入300μL Al~(3+)、Fe~(3+)時會產(chǎn)生聚集,加入EDTA后聚集現(xiàn)象消失。
[Abstract]:Gold nanoparticles au NPs) due to their unique optical, electrical, magnetic and other properties in the environment, drugs. Food and other fields are widely used. The simplest and most commonly used method to prepare AuNPs is sodium citrate reducing gold salt solution. However. The AuNPs prepared by this method is easy to gather in acidic or high ionic strength environment because of the change of surface charge of gold nanoparticles. It is a prerequisite for the wide application of AuNPs to maintain stability in complex environment. One of the effective methods to stabilize AuNPs is to add some modifiers to modify the surface of AuNPs. However, these modification methods still have some disadvantages. Calf thymus DNA has good biocompatibility, biodegradability and non-toxicity. It has been widely used in biological and medical fields. In this paper, the direct composite system of DNA-AuNPs was prepared by using calf thymus DNA as gold nano-stabilizer. The stability of the composite system in buffer solution with different pH value and different metal ion strength was studied according to the change of the color of the solution and the change of UV absorption peak. The effects of exogenous factors on the stability and reversible aggregation behavior of DNA-AuNPs direct composite system were preliminarily discussed. The main work of this thesis was as follows:. Effect of pH value on Stability and reversible aggregation behavior of DNA-AuNPs Direct Composite system. UV-Vis absorption Spectroscopy (UV-Vis). UV-Vis. The AuNPs solution was characterized by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The direct composite system of DNA-AuNPs can exist stably in buffer solution of pH 2-11. When there is a large amount of H in the solution, the direct composite system of DNA-AuNPs can aggregate. After addition of OH-, there was no deaggregating behavior, and the freezing and thawing cycle was not observed. The stability of AuNPs modified by DNA is stronger than that of AuNPs solution. The effect of metal ions on the stability and reversible aggregation behavior of DNA-AuNPs direct composite system is studied. DNA-AuNPs has good stability in the presence of a certain amount of 0 ~ 300 渭 L) low valence metal ion Na ~ (2 +) K ~ (2 +). However, when 300 渭 L Al~(3 was added, the aggregation would occur, and the aggregation would disappear after the addition of EDTA.
【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB383.1

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本文編號：1404159