本文關(guān)鍵詞：埃洛石納米管的小鼠毒性研究　出處：《中國(guó)科學(xué)技術(shù)大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： HNTs 體內(nèi)毒性 生物分布 氧化應(yīng)激 組織病理

【摘要】：埃洛石(Al_2Si_2O_5(OH)_4·nH_2O)是一種具有中空管狀結(jié)構(gòu)的天然無(wú)機(jī)納米粒子。因其豐富的產(chǎn)量、低廉的價(jià)格和獨(dú)特的納米結(jié)構(gòu),埃洛石被廣泛應(yīng)用于各個(gè)領(lǐng)域,比如陶瓷材料、高分子材料、污水處理。近年來(lái),埃洛石作為化妝品添加劑、藥物載體、超聲造影劑、牙齒填充劑和骨植入劑的探索成為熱門(mén)研究。在這些新的領(lǐng)域應(yīng)用埃洛石之前,需要對(duì)其安全性進(jìn)行評(píng)估。目前,人們對(duì)埃洛石的安全性沒(méi)有進(jìn)行系統(tǒng)評(píng)估。在已有的文獻(xiàn)中,埃洛石的體外毒性研究主要針對(duì)細(xì)胞和微生物;而體內(nèi)毒性方面,只有一篇文獻(xiàn)報(bào)道了埃洛石對(duì)線蟲(chóng)的毒性。本文采用由超聲破碎及粘度梯度分離法獲得的長(zhǎng)度可控的高純埃洛石,研究了 30天重復(fù)灌胃給藥后埃洛石對(duì)小鼠的毒性作用。主要包括以下內(nèi)容:第一章,首先介紹了納米材料的特點(diǎn)和影響納米材料毒性的因素及其主要入侵途徑,然后概述了埃洛石納米管的特性和其在各個(gè)領(lǐng)域的廣泛應(yīng)用,最后總結(jié)了埃洛石毒性研究的已有數(shù)據(jù),包括其體外毒性和體內(nèi)毒性研究。第二章,首先分別對(duì)純化前后的埃洛石納米管(HNTs)進(jìn)行了 TEM和XRF表征;然后探索了純化后的HNTs在人工胃液、腸液、血液中的溶解度,并用DLS測(cè)定其水動(dòng)力學(xué)半徑;最后做了HNTs小鼠體內(nèi)毒性預(yù)實(shí)驗(yàn),2000 mg/kg BW劑量,灌胃給藥7天。研究表明HNTs對(duì)小鼠肺產(chǎn)生了嚴(yán)重毒性,包括Al大量聚集,肺纖維化,小鼠的肝臟和腎臟Al、Si含量增加,且發(fā)生了組織病理變化,其中肝臟為細(xì)胞水腫、點(diǎn)狀壞死和脂肪肝,腎臟為細(xì)胞水腫、腎小球萎縮。初步證明HNTs對(duì)小鼠的肺、肝、腎有毒性。第三章,研究了不同劑量(5,50,300 mg/kg BW)的HNTs對(duì)小鼠肺的毒性(30天)。結(jié)果證明5 mg/kg BW劑量對(duì)小鼠肺是安全的,沒(méi)有發(fā)生組織病變、Al元素聚集、氧化應(yīng)激。而50,300mg/kg BW劑量的HNTs引起肺部紅細(xì)胞滲出、肺泡上皮細(xì)胞增生、纖維化,還發(fā)生了Al大量聚集和HNTs顆粒沉積。第四章,研究不同劑量(5,50,300 mg/kg BW)的HNTs對(duì)小鼠肝、腎的毒性(30天)。實(shí)驗(yàn)結(jié)果表明5 mg/kg BW劑量的HNTs對(duì)小鼠肝、腎無(wú)顯著毒性,50,300 mg/kg BW劑量時(shí)HNTs會(huì)引起肝、腎的組織病變和功能障礙。同時(shí),我們發(fā)現(xiàn)低劑量的HNTs可促進(jìn)小鼠體重增長(zhǎng),高劑量對(duì)體重增長(zhǎng)有抑制作用。
[Abstract]:Al2Si2O5S / s / s / s / nH2Os are natural inorganic nanoparticles with hollow tubular structures, due to their rich output. With its low price and unique nanostructure, Ellostone has been widely used in various fields, such as ceramic materials, polymer materials, sewage treatment. In recent years, Ellostone has been used as cosmetic additive and drug carrier. The exploration of ultrasound contrast agents dental fillers and bone implants has become a hot topic. The safety of Ellostone needs to be evaluated before it can be used in these new areas. There has been no systematic assessment of the safety of Ellostone. In the literature, in vitro toxicity studies have been focused on cells and microbes; However, in vivo toxicity, only one document reported the toxicity of Elomite to nematodes. In this paper, the length controlled high purity Elosterite was obtained by ultrasonic crushing and viscosity gradient separation. The toxic effects of Ellostone on mice after repeated oral administration for 30 days were studied. The main contents were as follows: chapter 1. The characteristics of nanomaterials, the factors affecting the toxicity of nanomaterials and their main invasion approaches are introduced. Then, the characteristics of Ellosite nanotubes and their wide applications in various fields are summarized. Finally, the existing data of the study on the toxicity of Elomite are summarized, including in vitro and in vivo toxicity studies. Chapter 2. Firstly, TEM and XRF were used to characterize the HNTs before and after purification. Then, the solubility of purified HNTs in artificial gastric juice, intestinal fluid and blood was explored, and its hydrodynamic radius was determined by DLS. Finally, the HNTs mice in vivo toxicity pretest of 2000 mg/kg BW dose, intragastric administration of 7 days. The study showed that HNTs induced severe toxicity to the lungs of mice, including a large number of Al accumulation. With pulmonary fibrosis, the contents of Albumin Si in liver and kidney of mice increased, and histopathological changes occurred. The liver was cell edema, punctate necrosis and fatty liver, and kidney was cell edema. Glomerular atrophy. HNTs was proved to be toxic to lung, liver and kidney in mice. The results showed that the dose of 5 mg/kg BW was safe for the lung of mice, and no pathological changes occurred. The concentration of Al elements, oxidative stress, and 50 ~ 300mg / kg BW HNTs caused pulmonary erythrocyte exudation, alveolar epithelial cell proliferation, fibrosis. A large number of Al aggregations and HNTs particle deposition were also observed in chapter 4th. The effects of different doses of HNTs on the liver of mice were studied. The results showed that the dose of 5 mg/kg BW HNTs had no significant toxicity to the liver and kidney of mice. At the dose of 300 mg/kg BW, HNTs can cause liver and kidney lesions and dysfunction. At the same time, we found that low dose of HNTs can promote weight gain in mice. High doses have an inhibitory effect on weight gain.
【學(xué)位授予單位】：中國(guó)科學(xué)技術(shù)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TB383.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 牛繼南;強(qiáng)穎懷;王春陽(yáng);李祥;周一浩;商翔宇;莊全超;;埃洛石的命名、結(jié)構(gòu)、形貌和卷曲機(jī)制[J];礦物學(xué)報(bào);2014年01期

2 孫文君;周靈君;丁安偉;;礦物藥赤石脂的研究進(jìn)展[J];廣州化工;2010年11期

3 劉高魁;彭文世;;景德鎮(zhèn)幾種陶瓷礦物的紅外光譜[J];景德鎮(zhèn)陶瓷;1988年04期

，

本文編號(hào)：1401288