本文關(guān)鍵詞：氧化鋅納米晶合成表征及抗菌性能研究　出處：《內(nèi)蒙古工業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 氧化鋅納米晶 陰道模型 氧化應(yīng)激 最小抑菌濃度 抗菌活性

【摘要】：近幾十年來,抗生素在醫(yī)療領(lǐng)域、養(yǎng)殖領(lǐng)域的大量過度使用,使本已嚴(yán)重的耐藥問題日益惡化。超級細(xì)菌的出現(xiàn)更是為人們敲響了警鐘,同時(shí)對抗菌制劑提出了更高的要求。目前科研人員開始轉(zhuǎn)向?qū)ふ倚滦偷目鼓退幮詮?qiáng),廣譜抗菌而且生物相容性良好的抗菌制劑。無機(jī)納米材料作為一類新型抗菌劑,具有抗耐藥性強(qiáng)、廣譜抗菌等優(yōu)勢,近年來受到人們的普遍關(guān)注,其中,氧化鋅作為無機(jī)納米材料重要組成部分,在抗菌領(lǐng)域已經(jīng)得到了一定的應(yīng)用。然而,利用簡單廉價(jià)的方法合成單分散性和生物相容性良好的小尺寸氧化鋅納米晶仍存在困難。同時(shí),目前有關(guān)氧化鋅納米晶的抗菌應(yīng)用多集中于體外研究,將其應(yīng)用于體內(nèi)抗菌的報(bào)道很少。本文利用小分子配體低溫溶劑合成方法合成了小尺寸氧化鋅,并首次對其生物安全性及其體內(nèi)外抗菌活性進(jìn)行了評價(jià),主要內(nèi)容包括:一、采用小分子配體低溫溶劑熱法合成水中穩(wěn)定及單分散性良好的小尺寸氧化鋅納米晶。利用TEM,XRD等手段對其超微結(jié)構(gòu)、尺寸、成分、表面性質(zhì)及其光學(xué)性能進(jìn)行表征評價(jià)。二、評價(jià)所制備氧化鋅納米晶對Hep G-2細(xì)胞的生物相容性及其導(dǎo)致細(xì)胞凋亡機(jī)理。同時(shí)考察氧化鋅納米晶在小鼠模型上的急性毒性表現(xiàn),結(jié)果表明氧化鋅納米晶對Hep G-2細(xì)胞具有一定的毒性。納米晶在高劑量時(shí)會(huì)導(dǎo)致小鼠死亡,但與先前報(bào)道相比,所制備氧化鋅具有良好生物相容性和生物安全性。三、以大腸桿菌和金黃色葡萄球菌為檢測模型,評價(jià)氧化鋅納米晶抑菌效果。抑菌圈實(shí)驗(yàn)和最小抑菌濃度(MIC)結(jié)果表明所制備氧化鋅納米晶擁有優(yōu)良的抑菌效果。為了進(jìn)一步評價(jià)氧化鋅作為抗菌制劑在醫(yī)學(xué)領(lǐng)域的潛在應(yīng)用,本文以大腸桿菌誘導(dǎo)的小鼠陰道炎為模型,考察納米氧化鋅復(fù)合PVA對該感染模型的治療效果。結(jié)果表明,氧化鋅對大腸桿菌所引起的小鼠陰道炎具有良好的治療效果,說明納米氧化鋅作為抗菌制劑在醫(yī)學(xué)抗感染領(lǐng)域具有巨大的應(yīng)用前景。綜上所述,本文主要介紹了以一種嶄新的方法合成小尺寸氧化鋅納米晶,并系統(tǒng)研究其生物相容性,抗菌活性及其在細(xì)菌抗感染模型上的應(yīng)用,本文合成氧化鋅納米晶展示優(yōu)越的抗菌性能和良好的生物相容性。這為納米氧化鋅進(jìn)一步走向臨床應(yīng)用提供了重要的實(shí)驗(yàn)基礎(chǔ)和理論指導(dǎo)。
[Abstract]:In recent decades, antibiotics in the medical field, a large number of breeding areas of excessive use, making the already serious problem of drug resistance is deteriorating. The super bacteria is a wake-up call for people at the same time, put forward higher requirements on antimicrobial agents. At present, researchers began to seek a new anti strong drug resistance, antibacterial good biocompatibility and antibacterial agents. The inorganic nano materials as a new type of antibacterial agent, anti drug resistance, antibacterial and other advantages, is widely concerned by people in recent years, including Zinc Oxide, as an important part of inorganic nano materials, has been widely used in the field of antimicrobial. However, the method is simple and cheap the synthesis of monodisperse nano crystalline Zinc Oxide compatible with small size and good biological difficulties still exist. At the same time, the antibacterial nano crystal with the Zinc Oxide focus In vitro study, its application in the in vivo antibacterial is rarely reported. In this paper, the small size of Zinc Oxide was synthesized by using small molecule ligands at low temperature solvent synthesis method, and for the first time on the biological safety and in vivo antibacterial activity was evaluated. The main contents include: first, using small molecule ligands low-temperature solvothermal synthesis and water stability good dispersion of small size Zinc Oxide nanocrystals. The use of TEM, XRD and other means on the ultrastructure, size, composition, characterization of surface properties and optical properties of the evaluation. Two, evaluation of the prepared nanocrystals of Hep Zinc Oxide G-2 cell biocompatibility and leads to apoptosis mechanism. At the same time to study acute toxicity of Zinc Oxide nanocrystals in a mouse model of performance, results show that Zinc Oxide nanocrystalline has certain toxicity to Hep cells. G-2 nanocrystals can lead to the death of mice in high dose, but with the previously reported Over, the prepared Zinc Oxide has compatibility and good biological safety. Biological three, Escherichia coli and Staphylococcus aureus as the detection model, to evaluate the effect of nano antibacterial. Zinc Oxide antibacterial assay and minimum inhibitory concentration (MIC) results indicated that the prepared nanocrystals have Zinc Oxide excellent antibacterial effect to. Further evaluation of Zinc Oxide as an antimicrobial potential application in the field of medicine preparation, mice taking vaginitis induced by Escherichia coli as a model to investigate the therapeutic effect of nano PVA on the Zinc Oxide composite infection model. The results showed that the mice caused by vaginitis Zinc Oxide on Escherichia coli has a good therapeutic effect, nano antibacterial agents has application prospect as Zinc Oxide giant in the field of anti infective medicine. To sum up, this paper mainly introduces a new method for the synthesis of small size nano Zinc Oxide Crystal, and study its biocompatibility, antibacterial activity and anti bacterial infection in the application model, this paper synthesized nanocrystals show Zinc Oxide compatibility and good antibacterial performance. The biological nano Zinc Oxide further clinical application provides an important experimental basis and theoretical guidance.

【學(xué)位授予單位】：內(nèi)蒙古工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1;O614.241

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相關(guān)期刊論文 前2條

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本文編號：1376045