【摘要】：研究目的:探討6周持續(xù)與間歇跑臺(tái)運(yùn)動(dòng)對(duì)大鼠血清及不同組織(心肌,肝臟,股四頭肌,腓腸肌,比目魚肌)MDA含量變化及SOD、T-AOC活性變化的影響。研究方法:雄性SD大鼠24只,隨機(jī)分為安靜對(duì)照組(S組)、持續(xù)訓(xùn)練組(C組)和長時(shí)間歇訓(xùn)練組(I組),共3組,每組8只。運(yùn)動(dòng)組先進(jìn)行為期1周的適應(yīng)性跑臺(tái)。適應(yīng)性跑臺(tái)訓(xùn)練按照以下3級(jí)負(fù)荷進(jìn)行:(1)第1-2天以15m/min×20min;(2)第3-4天18m/min×20min;(3)第5-6天21m/min×20min。正式訓(xùn)練時(shí),安靜組不運(yùn)動(dòng),正常飼養(yǎng),持續(xù)6周。運(yùn)動(dòng)組第一周以22m/min的速度開始進(jìn)行跑臺(tái)訓(xùn)練,以后每周以1m/min的速度遞增,直至第6周27m/min為止,6天/周。其中持續(xù)訓(xùn)練組每天訓(xùn)練一次,訓(xùn)練40min;長時(shí)間歇組每天訓(xùn)練2組,每組訓(xùn)練20min,組間休息3h。6周訓(xùn)練結(jié)束后不限飲水,禁食24h,處死分別取出心尖血5ml,心肌,肝臟,股四頭肌,比目魚肌,腓腸肌等組織置于-80℃冰箱待測(cè),全程在冰上進(jìn)行,最后采用比色法(試劑盒)測(cè)定大鼠的MDA、SOD、T-AOC等指標(biāo)。研究結(jié)果:1.MDA變化:6周持續(xù)與間歇跑臺(tái)運(yùn)動(dòng)后,持續(xù)運(yùn)動(dòng)組血清MDA含量為5.7±0.49nmol/ml,與安靜對(duì)照組相比顯著降低(P0.05),長時(shí)間歇運(yùn)動(dòng)組血清MDA含量為3.65±0.68nmol/ml,與安靜組相比有著極顯著的差異(P0.01),且長時(shí)間歇組與持續(xù)訓(xùn)練組相比呈現(xiàn)出了顯著差異(P0.05);心肌MDA含量持續(xù)訓(xùn)練組與安靜對(duì)照組相比無明顯差異(P0.05),長時(shí)間歇組與安靜對(duì)照組相比,差異顯著(P0.05),長時(shí)間歇組與持續(xù)組相比,顯著下降(P0.05);肝臟MDA含量長時(shí)間歇組與安靜對(duì)照組相比明顯降低(P0.01),長時(shí)間歇組與持續(xù)訓(xùn)練組相比差異極顯著(P0.01);股四頭肌和腓腸肌MDA含量較安靜組相比,有下降的趨勢(shì),但差異性分析無統(tǒng)計(jì)學(xué)意義(P0.05)而比目魚肌MDA含量持續(xù)組與對(duì)照組相比顯著降低(P0.05),長時(shí)間歇組與對(duì)照組相比降低的趨勢(shì)極顯著(P0.01),且長時(shí)間歇組與持續(xù)組相比也呈現(xiàn)出顯著下降趨勢(shì)(P0.05)。2.SOD變化:運(yùn)動(dòng)組大鼠血清SOD的數(shù)值有上升的趨勢(shì),持續(xù)組與對(duì)照組相比差異性不顯著(P0.05)長時(shí)間歇組與對(duì)照組和持續(xù)組相比差異極顯著(P0.01)。心肌SOD值運(yùn)動(dòng)組與安靜組相比有升高的趨勢(shì),且長時(shí)間歇組與對(duì)照組相比有極顯著差異(P0.01),與持續(xù)組相比,也呈現(xiàn)出顯著差異(P0.05)。肝臟SOD值兩運(yùn)動(dòng)組與安靜對(duì)照組相比,有顯著性的差異(P0.01);長時(shí)間歇組與持續(xù)組相比差異極其顯著(P0.01)。股四頭肌和腓腸肌SOD含量兩運(yùn)動(dòng)組與對(duì)照組相比呈現(xiàn)上升趨勢(shì),但無統(tǒng)計(jì)學(xué)差異(P0.05),而比目魚肌SOD含量長時(shí)間歇組與對(duì)照組和持續(xù)組相比差異顯著(P0.05)。3.T-AOC變化:持續(xù)組和長時(shí)間歇組血清T-AOC活性與安靜組相比明顯升高,且差異性顯著(P0.05和P0.01);心肌T-AOC活性長時(shí)間歇組與安靜對(duì)照組和持續(xù)組相比有顯著差異(P0.01和P0.05),持續(xù)組與對(duì)照組相比無統(tǒng)計(jì)學(xué)差異(P0.05);肝臟T-AOC活性兩運(yùn)動(dòng)組與安靜組相比,活性明顯增強(qiáng),具有極顯著的差異(P0.01),且長時(shí)間歇組與持續(xù)組相比也具有顯著性差異(P0.05);股四頭肌和腓腸肌T-AOC活性與安靜組相比有所升高,但沒有顯著性差異(P0.05);比目魚肌T-AOC活性運(yùn)動(dòng)組與安靜組相比有明顯增強(qiáng)的趨勢(shì)(P0.05),持續(xù)組與長時(shí)間歇組相比無統(tǒng)計(jì)學(xué)意義(P0.05)。研究結(jié)論:(1)經(jīng)過6周持續(xù)與間歇跑臺(tái)訓(xùn)練后,兩種不同運(yùn)動(dòng)方式的大鼠體重增長幅度顯著低于安靜對(duì)照組,且長時(shí)間歇組顯著低于持續(xù)組,推測(cè)中等強(qiáng)度的長時(shí)間歇有氧運(yùn)動(dòng)有助于體重控制。(2)經(jīng)過6周持續(xù)與間歇跑臺(tái)運(yùn)動(dòng)訓(xùn)練后,持續(xù)組與長時(shí)間歇組血清,心肌,肝臟和骨骼肌自由基MDA的含量較對(duì)照組呈現(xiàn)下降趨勢(shì),兩運(yùn)動(dòng)組SOD和T-AOC的活性較安靜組均呈現(xiàn)增強(qiáng)趨勢(shì),提示適宜的運(yùn)動(dòng)有助于提高機(jī)體的抗氧化能力。(3)經(jīng)過6周持續(xù)與間歇跑臺(tái)訓(xùn)練后,長時(shí)間歇訓(xùn)練組比目魚肌SOD、T-AOC的活性顯著高于股四頭肌和腓腸肌,推測(cè)骨骼肌自由基的代謝可能與肌纖維類型有關(guān)。(4)在運(yùn)動(dòng)量相同的前提下,經(jīng)過6周持續(xù)與間歇跑臺(tái)訓(xùn)練后,長時(shí)間歇訓(xùn)練組血清和心肌自由基的代謝顯著優(yōu)于持續(xù)組和對(duì)照組,提示長時(shí)間歇訓(xùn)練組更利于自由基代謝。(5)在運(yùn)動(dòng)量相同的前提下,經(jīng)過6周持續(xù)與間歇跑臺(tái)訓(xùn)練后,長時(shí)間歇訓(xùn)練組肝臟自由基的代謝顯著優(yōu)于持續(xù)組和對(duì)照組,提示長時(shí)間歇訓(xùn)練有利于提高機(jī)體肝臟的解毒能力。
[Abstract]:Objective: To study the effects of 6-week continuous and intermittent treadmill exercise on the changes of MDA content and the activity of SOD, T-AOC in rat serum and different tissues (myocardium, liver, quadriceps femoris, gastrocnemius, and flounder). Methods:24 male SD rats were randomly divided into a quiet control group (S group), a continuous training group (C group) and a long-time rest training group (group I). The exercise group first carries out a 1-week adaptive run. The training of the adaptive running table is carried out according to the following 3-level load: (1) the first-2 days are at 15 m/ min to 20 min; (2) the day 3-4 is 18 m/ min for 20 min; and (3) the day 5-6 is 21 m/ min to 20 min. In formal training, the quiet group does not move, and is normally fed for 6 weeks. In the first week of the exercise group, the treadmill exercise was started at a speed of 22 m/ min, and then increased at a rate of 1 m/ min after a week, up to 27 m/ min at Week 6 and 6 days/ week. wherein, the continuous training group is trained once a day and is trained for 40 minutes; the long-time rest group is used for training two groups on a daily basis, each group is trained for 20 minutes, the groups rest for 3 hours; after the 6-week training is finished, the water is not allowed to drink, and the heart muscle, the liver, the quadriceps femoris and the flounder are taken out, The tissue of gastrocnemius was placed in-80 鈩，

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