發(fā)布時(shí)間：2018-07-03 05:40

  本文選題：有氧運(yùn)動 + 腦衰老��； 參考：《成都體育學(xué)院》2017年碩士論文

【摘要】：目的:通過觀察有氧運(yùn)動對SD大鼠衰老模型空間學(xué)習(xí)記憶能力及額葉腦源性神經(jīng)營養(yǎng)因子(BDNF)表達(dá)的影響,探討有氧運(yùn)動對大鼠腦衰老的影響及作用機(jī)理。方法:3月齡SD雄性大鼠共54只,隨機(jī)分成6組:生理鹽水注射對照組(NNa組)、D-半乳糖注射致衰老組(ND組)、D-半乳糖注射致衰老前運(yùn)動組(SD組)、D-半乳糖注射致衰老過程中運(yùn)動組(NDs組)、D-半乳糖注射致衰老后運(yùn)動組(NDS組)以及D-半乳糖注射致衰老后自然喂養(yǎng)組(NDN),每組9只(n=9)。SD組于衰老造模前進(jìn)行4周有氧運(yùn)動干預(yù),NDs組于衰老造模過程中進(jìn)行6周有氧運(yùn)動干預(yù),NDS組于衰老造模后進(jìn)行4周有氧運(yùn)動干預(yù),NDN組于衰老造模后不進(jìn)行運(yùn)動干預(yù)。選用Morris水迷宮系統(tǒng)訓(xùn)練并檢測各組大鼠定位航行與空間探索能力。實(shí)驗(yàn)結(jié)束之后,斷頭處死,分離大鼠額葉。檢測不同衰老時(shí)期大鼠超氧化物歧化酶(SOD)、谷胱甘肽過氧化酶(GSH-PX)的活性以及丙二醛(MDA)的含量。應(yīng)用免疫組化、Real time PCR以及Westren-Blotting技術(shù)分別檢測額葉BDNF基因mRNA和蛋白的表達(dá)。結(jié)果:1.自由基檢測結(jié)果:ND組比NNa組GSH-PX和SOD的活性顯著降低(P0.05),MDA的含量顯著增加(P0.05);NDs組比ND組GSH-PX和SOD的活性顯著增加(P0.05),MDA的含量顯著降低(P0.05);SD組比ND組SOD、GSH-PX的活性以及MDA的含量均未出現(xiàn)顯著性變化(P0.05);NDS組比NDN組GSH-PX和SOD的活性顯著增加(P0.05),MDA的含量顯著降低(P0.05);NDN組比ND組GSH-PX和SOD的活性也未出現(xiàn)顯著的差異(P0.05),MDA的含量顯著增加(P0.05);NDs組比NDS組GSH-PX和SOD的活性顯著升高(P0.01),MDA的含量顯著降低(P0.01)。2.空間學(xué)習(xí)能力檢測結(jié)果:(1)平臺穿越次數(shù):ND組比NNa組穿越原站臺次顯著減少(P0.05);NDs組比ND組顯著增加(P0.05),SD組與ND組之間未出現(xiàn)顯著的差異(P0.05);NDN組與NDS組也未出現(xiàn)顯著的差異(P0.05)。(2)原平臺象限的游泳路程與總路程的比值:ND組顯著低于NNa組(P0.01);NDs組顯著高于ND組(P0.01),SD組與ND組無顯著差異(P0.05);NDN組與NDS組也沒有顯著差異(P0.05)。3.BDNF表達(dá)情況:ND組比NNa組BDNF蛋白表達(dá)量顯著下調(diào)(P0.05);NDs組比ND組BDNF mRNA及蛋白表達(dá)量均顯著下調(diào)(P0.01),SD組BDNF mRNA及蛋白表達(dá)量與ND組相比未出現(xiàn)顯著差異(P0.05)。結(jié)論:1.通過6周D-半乳糖注射成功構(gòu)建了亞急性衰老大鼠模型。2.腦衰老過程中進(jìn)行有氧運(yùn)動干預(yù)可以提高大鼠的學(xué)習(xí)記憶能力,上調(diào)額葉BDNF的表達(dá),說明衰老過程中進(jìn)行有氧運(yùn)動干預(yù)能夠延緩腦衰老。3.額葉BDNF蛋白表達(dá)量與學(xué)習(xí)記憶呈線性正相關(guān),提示額葉BDNF表達(dá)量的上調(diào)是改善學(xué)習(xí)記憶能力的機(jī)制之一。
[Abstract]:Objective: to observe the effects of aerobic exercise on the spatial learning and memory ability and the expression of BDNF in the frontal lobe of SD rats, and to explore the effect and mechanism of aerobic exercise on brain aging in SD rats. Methods 54 male Sprague-Dawley rats aged 3 months were selected. The rats were randomly divided into 6 groups: normal saline injection control group (NNa group), D-galactose injection induced senescence group (ND group), D-galactose injection induced aging exercise group (SD group), D-galactose injection induced senescence process, exercise group (NDs group), D-galactose injection induced senescence. Elderly exercise group (NDS group) and natural feeding group (NDN) induced by D-galactose injection. Nine rats in each group (nun9). SD group were treated with aerobic exercise for 4 weeks before aging model making. NDs group underwent 6 weeks aerobic exercise intervention during aging model making process. Aerobic exercise intervention was performed for 4 weeks after aging model making. No exercise intervention was carried out in NDN group after aging modeling. Morris water maze system was used to train and measure the ability of navigation and space exploration. After the experiment, the rats were killed and the frontal lobes were separated. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) in different aging rats were determined. The expression of BDNF gene mRNA and protein in frontal lobe was detected by immunohistochemistry real time PCR and Westren-Blotting respectively. The result is 1: 1. Results the activity of GSH-PX and SOD decreased significantly (P0.05) the activity of GSH-PX and SOD in NDs group was significantly lower than that in ND group (P0.05), the activity of GSH-PX and SOD in NDs group was significantly higher than that in ND group (P0.05), the activity of GSH-PX and MDA in SD group was significantly lower than that in ND group (P0.05) and the content of MDA in SD group was not significantly higher than that in ND group. The activity of GSH-PX and SOD in NDS group was significantly higher than that in NDN group (P0.05) the content of MDA in NDS group was significantly lower than that in NDN group (P0.05) there was no significant difference in GSH-PX and SOD activity between NDN group and ND group (P0.05). (P0.05) the activity of GSH-PX and SOD in NDS group was significantly higher than that in NDS group (P0.05). The content of MDA decreased significantly (P0.01). The results of spatial learning ability test: (1) the number of platform traversing through the original platform was significantly lower in the N _ ND group than in the NNA group (P0.05). (P0.05) there was no significant difference between the SD group and the ND group (P0.05). There was no significant difference between the NDN group and the NDS group. (P0.05) there was no significant difference between the NDN group and the NDS group. (P0.05) there was no significant difference between the SD group and the ND group (P0.05). (P0.05) the ratio of swimming distance to total distance in the original platform quadrant in ND group was significantly lower than that in NNa group (P0.01). There was no significant difference between SD group and ND group (P0.05). The expression of BDNF mRNA and protein in NDs group was significantly lower than that in ND group (P0.01). There was no significant difference in BDNF mRNA and protein expression between SD group and ND group (P0.05). Conclusion 1. Subacute aging rat model was successfully established by injection of D-galactose at 6 weeks. Aerobic exercise intervention during brain aging can improve the learning and memory ability of rats and up-regulate the expression of BDNF in frontal lobe, indicating that aerobic exercise intervention during aging can delay brain aging. 3. The expression of BDNF protein in frontal lobe was positively correlated with learning and memory, suggesting that the up-regulation of BDNF expression in frontal lobe is one of the mechanisms to improve learning and memory ability.
【學(xué)位授予單位】：成都體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.7

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