本文選題：運(yùn)動(dòng) + 非酒精性脂肪肝�。� 參考：《成都體育學(xué)院》2016年碩士論文

【摘要】：目的:觀察8周游泳運(yùn)動(dòng)對(duì)非酒精性脂肪肝(NAFLD)大鼠生殖細(xì)胞凋亡以及Fas/FasL、Caspase-3表達(dá)的影響,探討有氧運(yùn)動(dòng)干預(yù)NAFLD大鼠生殖細(xì)胞凋亡的可能機(jī)制。方法:50只成年雄性SD大鼠隨機(jī)分為對(duì)照組(C組,n=20)和模型組(M組,n=30)兩組;C組再分為8周對(duì)照組(C8)和16周對(duì)照組(C16),M組又分為8周模型組(M8)、16周模型組(M16)及運(yùn)動(dòng)模型組(EM)。C組基礎(chǔ)飼料飼養(yǎng),M組給予高脂飼料飼養(yǎng)8周以建立NAFLD大鼠模型。8周后C8、M8兩組禁食12小時(shí)處死,取肝組織HE染色光鏡下行組織病理學(xué)觀察,檢測(cè)血TG、TC、AST、ALT含量,評(píng)定NAFLD大鼠模型成功率;第9周開(kāi)始,EM組進(jìn)行為期8周(60min/d,6d/w)無(wú)負(fù)重游泳訓(xùn)練。運(yùn)動(dòng)結(jié)束后處死C16、M16、EM三組大鼠,檢測(cè)血TG、TC、AST、ALT含量,取肝組織、睪丸組織及睪周脂肪分別稱(chēng)重記錄保存,HE染色光鏡下觀察肝組織、睪丸組織病理形態(tài);采用原位末端轉(zhuǎn)移酶標(biāo)記法(TUNEL法)標(biāo)記睪丸生殖細(xì)胞的凋亡情況;Western blot法檢測(cè)各組大鼠睪丸組織Fas/FasL、Caspase-3蛋白表達(dá)情況。結(jié)果:(1)C8組未見(jiàn)明顯肝細(xì)胞脂肪性變,M8組8只出現(xiàn)脂肪性變,M8組相對(duì)C8組肝細(xì)胞脂肪變程度積分顯著增高;C16組2只可見(jiàn)肝細(xì)胞內(nèi)少量脂肪性變,M16組全部大鼠脂肪性變明顯,EM組中(n=9)6只可見(jiàn)脂肪性變;肝細(xì)胞脂肪變程度積分M16、EM組顯著高于C16組,EM組顯著低于M16組。(2)M8組血清AST、ALT、TC、TG較C8組顯著增高;與C16組比較,M16組血清AST、ALT、TC、TG含量均顯著增高,EM組TC含量顯著增高,AST、ALT、TG含量雖有增高但組間比較無(wú)統(tǒng)計(jì)學(xué)差異。(3)M16和EM組睪周脂肪重量較C16組分別表現(xiàn)出輕度上升和下降,但組間差異無(wú)顯著性;M16和EM組睪丸系數(shù)較C16組分別表現(xiàn)出輕度下降和上升,但無(wú)統(tǒng)計(jì)學(xué)差異,EM組大鼠睪丸系數(shù)較M16組顯著上升。(4)M16組大鼠生精小管排列較疏松,生精細(xì)胞形態(tài)、排列異常;EM組大鼠生精小管較M16組排列緊密,生精細(xì)胞形態(tài)、排列未見(jiàn)明顯異常。(5)與C16組比較,M16組睪丸生殖細(xì)胞凋亡明顯增加,Fas/FasL、Caspase-3蛋白表達(dá)均顯著上升,EM組凋亡程度增高,Fas/FasL、Caspase-3表達(dá)上調(diào),但組間無(wú)顯著差異;與M16組比較,EM組睪丸生殖細(xì)胞凋亡顯著較少,Fas/FasL、Caspase-3蛋白表達(dá)均顯著下降。結(jié)論:(1)高脂膳食可誘導(dǎo)SD大鼠NAFLD的形成,且隨著高脂飼養(yǎng)時(shí)間的延長(zhǎng),NAFLD發(fā)生率上升,程度逐步加重。(2)NAFLD大鼠睪丸生殖細(xì)胞凋亡增多,而睪丸組織Fas/FasL、Caspase-3蛋白含量升高是引起凋亡增加的重要原因。(3)8周游泳運(yùn)動(dòng)明顯減輕大鼠NAFLD程度,同時(shí)顯著降低NAFLD大鼠睪丸生殖細(xì)胞凋亡程度,下調(diào)Fas/FasL、Caspase-3蛋白表達(dá)是其可能機(jī)制之一。
[Abstract]:Aim: to observe the effects of swimming for 8 weeks on the apoptosis of germ cells and the expression of Fas-FasL caspase-3 in rats with nonalcoholic fatty liver disease (NAF LDD), and to explore the possible mechanism of aerobic exercise on the apoptosis of germ cells in NAFLD rats. Methods Fifty adult male Sprague-Dawley rats were randomly divided into two groups: control group (C) and model group (n = 30). Group C was further divided into control group (n = 8) and control group (n = 16). Group M was fed with high fat diet for 8 weeks to establish NAFLD rat model. After 8 weeks, two groups were killed by fasting for 12 hours. The liver tissues were stained with HE staining to observe the histopathological changes, to detect the alt content of TGG TCCA, to evaluate the success rate of NAFLD rat model, and to conduct weightless swimming training in EM group for 8 weeks (60 min / d ~ 6 d / w) from the 9th week. At the end of exercise, three groups of rats were killed. The contents of alt in blood TGV TCU ASTT were measured. The liver tissue, testis tissue and peritestis fat were measured by HE staining light microscope to observe the histopathology of the liver and testis. Apoptosis of testicular germ cells was detected by in situ terminal transferase labeling (Tunel) and Western blot method was used to detect the expression of Fas-P / FasL- Caspase-3 protein in rat testis. Results there was no obvious fatty degeneration of hepatocytes in C8 group. The fatty degeneration in M8 group was significantly higher than that in C8 group. There were 2 rats in C16 group and all rats in M16 group were found to have adipose degeneration in liver cells. In EM group, 6 cases were found to have adipose degeneration. The liver cell fatty degree score in M16 EM group was significantly higher than that in C16 group, and was significantly lower in M16 group than that in M16 group. Compared with C16 group, the serum levels of TCTG in group M 16 were significantly higher than those in group C 16. The TC content in EM group was significantly higher than that in group C 16, but there was no significant difference between the two groups. The weight of peritestis fat in group EM and EM group showed a slight increase and decrease, respectively, compared with C16 group. However, there was no significant difference in testicular coefficient between M16 and EM groups compared with C16 group. However, there was no significant difference in testicular coefficient between EM group and M16 group. The testicular coefficient of EM group was significantly higher than that of M16 group. The arrangement of seminiferous tubules and the morphology of spermatogenic cells in EM group were looser than those in M16 group. The seminiferous tubules in EM group were arranged more closely than those in M16 group, and the morphology of spermatogenic cells in EM group was higher than that in M16 group. Compared with C16 group, the apoptosis of testicular germ cells in M16 group increased significantly, and the expression of Fas-Fas-Fas-Caspase-3 protein increased significantly in the EM group, but there was no significant difference between the two groups. Compared with M16 group, testicular germ cell apoptosis in EM group was significantly lower than that in M16 group. Conclusion: high fat diet can induce the formation of NAFLD in SD rats, and the incidence rate of NAFLD increases with the prolongation of high fat feeding time, and the degree of apoptosis of testicular germ cells increases gradually. The increase of caspase-3 protein content in testicular tissue is an important reason for the increase of apoptosis. Swimming for 8 weeks can significantly reduce the degree of NAFLD in rats, and decrease the degree of apoptosis of germ cells in testis of NAFLD rats. The down-regulation of Fas-FasL- Caspase-3 protein expression is one of the possible mechanisms.
【學(xué)位授予單位】：成都體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：G861.1;G804.2

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