本文選題：高強(qiáng)度間歇運(yùn)動(dòng) + 淋巴細(xì)胞亞群��； 參考：《體育科學(xué)》2017年09期

【摘要】：目的:觀察一次急性高強(qiáng)度間歇運(yùn)動(dòng)(HIIT)對(duì)外周血T淋巴細(xì)胞分布與免疫應(yīng)答的影響。方法:20名健康青年男性進(jìn)行一次HIIT,分別于運(yùn)動(dòng)前、運(yùn)動(dòng)后即刻以及運(yùn)動(dòng)后60 min取靜脈血,用流式細(xì)胞術(shù)測(cè)定淋巴細(xì)胞亞群分布以及體外T淋巴細(xì)胞刺激實(shí)驗(yàn)(佛波醇乙酯/離子霉素)誘導(dǎo)的免疫應(yīng)答標(biāo)志物(CD69、CD40L、CD122、GARP、LAP、GITR、CTLA-4和CD39)相對(duì)表達(dá)量[用平均熒光強(qiáng)度(MFI)和陽性細(xì)胞率表示],免疫分析法檢測(cè)血漿應(yīng)激激素(皮質(zhì)醇、腎上腺素和去甲腎上腺素等)的變化。結(jié)果:17名受試者完成全部實(shí)驗(yàn)。1)運(yùn)動(dòng)后即刻淋巴細(xì)胞及其各亞群計(jì)數(shù)均顯著性升高(P0.05),運(yùn)動(dòng)后60 min下降(P0.05)。2)運(yùn)動(dòng)后即刻,淋巴細(xì)胞計(jì)數(shù)與血漿腎上腺素(r=0.84,P0.05)及去甲腎上腺素(r=0.62,P0.05)含量顯著正相關(guān)。3)運(yùn)動(dòng)后即刻,CD40L(CD3~+CD4~+和CD4~+CD45RO~+T+h)、CD122(CD3~+CD8~+和CD3~+CD8~(high)T_c)和LAP(CD4+CD25highCD127lowTreg)表達(dá)上調(diào)(P0.05),運(yùn)動(dòng)后60 min時(shí)CD40L和CD122恢復(fù)(P0.05),T淋巴細(xì)胞各亞群CD69以及Treg細(xì)胞LAP/GARP和CD39表達(dá)顯著性升高(P0.05)。結(jié)論:一次急性HIIT能夠:1)造成內(nèi)分泌-免疫系統(tǒng)暫時(shí)性擾動(dòng),兒茶酚胺升高誘導(dǎo)淋巴細(xì)胞各亞群在循環(huán)中重新分布并呈現(xiàn)雙相反應(yīng)特征;2)選擇性動(dòng)員Th細(xì)胞和CD8~(high)T細(xì)胞并增強(qiáng)外源性刺激誘導(dǎo)免疫應(yīng)答的能力,運(yùn)動(dòng)后恢復(fù)期具有免疫抑制作用的Treg被動(dòng)員,從而調(diào)節(jié)并重建T細(xì)胞穩(wěn)態(tài)。
[Abstract]:Aim: to observe the effect of acute high intensity intermittent exercise (HIITs) on T lymphocyte distribution and immune response in peripheral blood. Methods Twenty healthy young men were treated with HIIT.Venous blood was taken before, immediately after exercise and 60 min after exercise. Flow cytometry was used to determine the distribution of lymphocyte subsets and the relative expression levels of CD69, CD40, L2, GITRCTLA-4 and CD39, which were induced by T lymphocyte stimulation test (phorbol / ionomycin) [using average fluorescence intensity (MFI)] and positive. The expression of sex cell rate, immunoassay was used to detect the plasma stress hormone (cortisol, cortisol). Changes of epinephrine and norepinephrine, etc. Results the counts of lymphocytes and their subsets increased significantly immediately after exercise, and decreased at 60 min after exercise. Lymphocyte count was positively correlated with plasma adrenocephalin (0.84p 0.05) and norepinephrine (0.62) P0.05). (3) immediately after exercise, the expression of CD40L and CD122 recovered P0.05T lymphocytes, and CD40L and CD122 recovered P0.05T lymphocytes at 60 min after exercise. The expression of LAP/GARP and CD39 in CD69 and Treg cells increased significantly (P 0.05). Conclusion: an acute HIIT can cause a transient disturbance of the endocrine and immune system. The elevation of catecholamine induces the redistribution of lymphocyte subsets in circulation and presents a biphasic response characteristic. It selectively mobilizes Th cells and CD8~(high)T cells and enhances the ability of exogenous stimuli to induce immune response. Treg, which has immunosuppressive effect in convalescence after exercise, is mobilized to regulate and reconstruct T cell homeostasis.
【作者單位】： 河南師范大學(xué)體育學(xué)院;河南工程學(xué)院體育部;
【基金】：河南省高等學(xué)校青年骨干教師資助計(jì)劃項(xiàng)目(2015GGJS-034)
【分類號(hào)】：G804.2

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1 孫治平;體育運(yùn)動(dòng)對(duì)人體免疫功能的影響[J];淮南師范學(xué)院學(xué)報(bào);2002年03期

2 姜斌;官一鵬;;強(qiáng)化訓(xùn)練和過度訓(xùn)練的免疫應(yīng)答[J];沈陽體育學(xué)院學(xué)報(bào);2013年04期

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本文編號(hào)：1900500